Assessment of DNA Damage after Photodynamic Therapy Using a Metallophthalocyanine Photosensitizer

El-Hussein, Ahmed; Harith, M. A.; Abrahamse, Heidi

doi:10.1155/2012/281068

Cited by 31 publications

(29 citation statements)

References 32 publications

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“…The differences between the effects in the absence and the presence of hOGG1 enzyme after 5 h of PDT exposure showed a significant difference in the level of DNA damage indicating that PDT induced both SB/ALS and oxidized purines. This increased level of DNA damage following PDT exposure is similar to the finding of El-Hussein et al (36) who showed that DNA strand breakage increased significantly in three cancer cell lines in response to PDT. Interestingly, a consistent high background level of SB/ALS and 8-oxoGua was detected in all unexposed and exposed samples before and after light exposure.…”

Section: Discussionsupporting

confidence: 89%

“…This increased level of DNA damage following PDT exposure is similar to the finding of El‐Hussein et al . who showed that DNA strand breakage increased significantly in three cancer cell lines in response to PDT. Interestingly, a consistent high background level of SB/ALS and 8‐oxoGua was detected in all unexposed and exposed samples before and after light exposure.…”

Section: Discussionmentioning

confidence: 99%

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Light‐based methods for whole blood bacterial inactivation enabled by a recirculating flow system

Kim

Karbaschi

Cooke

et al. 2018

Photochem & Photobiology

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Light of certain wavelengths can be used to inactivate pathogens. Whole blood is opaque; thus, the penetration of light is reduced. Here, we overcame this limitation using a thin transparent tube that is illuminated from all angles. Three light-based techniques were evaluated: photodynamic therapy (PDT) using a 660-nm light and antibody-photosensitizer conjugates, ultraviolet, and violet light. We observed a reduction of 55-71% of Staphylococcus aureus after 5 h of exposure (starting concentration 10 CFU mL ) and an 88-97% reduction in methicillin-resistant Staphylococcus aureus (MRSA) (starting 10 CFU mL ). An 83-92% decrease for S. aureus and 98-99.9% decrease for MRSA were observed when combined with an immunocapture approach. Complete blood count with differential analysis did not reveal any significant changes in the blood cell numbers. Genotoxicity studies showed that violet and ultraviolet did not induce any significant level of single strand breaks and alkali labile sites in the peripheral blood mononuclear cells (PBMC). In contrast, ultraviolet did induce a very low level of cyclobutane pyrimidine dimers, a UV damage indicator. PDT generated a significant level of single strand breaks and 8-oxoGua in these cells. The approaches showed promise for whole blood pathogen inactivation with minimal collateral damage to PBMC.

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Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Light‐based methods for whole blood bacterial inactivation enabled by a recirculating flow system

Kim

Karbaschi

Cooke

et al. 2018

Photochem & Photobiology

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“…Significant success indicated nanomedicine enhancements could benefit Pc4-PDT. ZnPc-PDT: 274 Lung (A549) A comparative study indicated phototoxic responses and cell death Oesophageal (SNO) across all tissue types at differential concentrations despite the Breast (MCF-7)…”

Section: Treatment Of Multiple Cancer Tissue Types/geneticmentioning

confidence: 99%

Cancer Tissue Classification, Associated Therapeutic Implications and PDT as an Alternative

Horne

Cronjé

2017

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Carcinogenesis occurs via mutation of critical genes conferring enhanced survival and protection to the ensuing tissue. Current therapies in use garner success due to their specificity for certain intracellular targets. This particularity, whist beneficial in identifying tumorigenic from normal tissue states, is limited by the variations in geno/phenotypic profiles displayed between tumor tissue types. As such, tissue-specific therapeutic combinations and adjuvants are often required for adequate effect, but present symptomatic complications and occasionally generate secondary carcinogenesis displaying multi-drug resistance (MDR). An accumulation of research over the recent years has suggested that photodynamic therapy (PDT) with macrocycle photosensitizers are a promising alternative. Its administration method and toxicity mechanism present attractive features for potentially overcoming MDR cancers of multiple tissue origins with limited symptomatic onsets. Herein, we highlight these potentials as referenced against existing therapeutics and consider the impact of macrocycle-PDT for broad spectrum application regardless of tumorigenic resistance profiles.

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“…[1][2][3] ROS consistently exhibit antimicrobial and anticancer effects through severe damage to DNA and the cytoplasmic membrane. [3][4][5] It has been reported that aPDT rarely creates drug-resistant bacteria, which is an adverse effect of antibiotic therapy. 6 Since ROS exhibit a broad spectrum of antimicrobial activity, aPDT…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial photodynamic activity and cytocompatibility of Au<sub>25</sub>(Capt)<sub>18</sub> clusters photoexcited by blue LED light irradiation

Miyata¹,

Miyaji²,

Kawasaki³

et al. 2017

IJN

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Antimicrobial photodynamic therapy (aPDT) has beneficial effects in dental treatment. We applied captopril-protected gold (Au 25 (Capt) 18 ) clusters as a novel photosensitizer for aPDT. Photoexcited Au clusters under light irradiation generated singlet oxygen ( 1 O 2 ). Accordingly, the antimicrobial and cytotoxic effects of Au 25 (Capt) 18 clusters under dental blue light-emitting diode (LED) irradiation were evaluated. 1 O 2 generation of Au 25 (Capt) 18 clusters under blue LED irradiation (420–460 nm) was detected by a methotrexate (MTX) probe. The antimicrobial effects of photoexcited Au clusters (0, 5, 50, and 500 μg/mL) on oral bacterial cells, such as Streptococcus mutans, Aggregatibacter actinomycetemcomitans , and Porphyromonas gingivalis , were assessed by morphological observations and bacterial growth experiments. Cytotoxicity testing of Au clusters and blue LED irradiation was then performed against NIH3T3 and MC3T3-E1 cells. In addition, the biological performance of Au clusters (500 μg/mL) was compared to an organic dye photosensitizer, methylene blue (MB; 10 and 100 μg/mL). We confirmed the 1 O 2 generation ability of Au 25 (Capt) 18 clusters through the fluorescence spectra of oxidized MTX. Successful application of photoexcited Au clusters to aPDT was demonstrated by dose-dependent decreases in the turbidity of oral bacterial cells. Morphological observation revealed that application of Au clusters stimulated destruction of bacterial cell walls and inhibited biofilm formation. Aggregation of Au clusters around bacterial cells was fluorescently observed. However, photoexcited Au clusters did not negatively affect the adhesion, spreading, and proliferation of mammalian cells, particularly at lower doses. In addition, application of Au clusters demonstrated significantly better cytocompatibility compared to MB. We found that a combination of Au 25 (Capt) 18 clusters and blue LED irradiation exhibited good antimicrobial effects through 1 O 2 generation and biosafe characteristics, which is desirable for aPDT in dentistry.

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Assessment of DNA Damage after Photodynamic Therapy Using a Metallophthalocyanine Photosensitizer

Cited by 31 publications

References 32 publications

Light‐based methods for whole blood bacterial inactivation enabled by a recirculating flow system

Light‐based methods for whole blood bacterial inactivation enabled by a recirculating flow system

Cancer Tissue Classification, Associated Therapeutic Implications and PDT as an Alternative

Antimicrobial photodynamic activity and cytocompatibility of Au<sub>25</sub>(Capt)<sub>18</sub> clusters photoexcited by blue LED light irradiation

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