Assessment of Disease Activity by Gallium-67 Scan and Lung Lavage in the Pneumoconioses

Bégin, Raymond; Bisson, Guy; Boileau, Robert; Massé, S.

doi:10.1055/s-2007-1012622

Cited by 34 publications

(7 citation statements)

References 25 publications

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“…Also, it clearly documented that dying sheep (group E) had significant deterioration of most parameters of disease, with clear increases in BAL neutrophils, fibronectin, and LDH enzyme, abnormalities that we have previously reported in progressive asbestosis [7], in fibrosing alveolitis, and in acute bacterial pneumonia [21]. To further document the effect of cyclophosphamide treatment on worsening the fibrotic lung disease Exp Lung Res Downloaded from informahealthcare.com by Chulalongkorn University on 12/27/14…”

Section: Resultsmentioning

confidence: 83%

Effects of Cyclophosphamide Treatment in Experimental Asbestosis

Bégin

Cantin

Massé

et al. 1988

Experimental Lung Research

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The potential role of immunosuppressive therapy in asbestosis was evaluated in the sheep model of experimental asbestosis. A diffuse peribronchiolar alveolar and interstitial fibrosing alveolitis was developed in 10 animals following 2 years of exposure consisting of slow intratracheal infusion of 100 mg Canadian chrysotile in 100 ml saline every 2 weeks. A control group of 10 sheep concomitantly receiving only 100 ml saline intratracheally was also enrolled in the study. One group of 5 control sheep and one group of 5 asbestosis sheep received 1 mg/kg cyclophosphamide in 10 ml saline iv every 2 weeks, the other 10 sheep receiving only saline. One year after beginning of therapy, survival rates were comparable in the 2 control groups and the asbestosis group without therapy at 80%, whereas it was significantly reduced at 20% in the asbestosis group with therapy. Deaths in the latter were associated with significant increase in peripheral blood and lung lavage neutrophils, increased intensity of fibrosing alveolitis, worsening of lung functions, and worsening in the radiographic diffuse lung opacities. This was documented on histopathology to be associated with more intense fibrotic disease and bacterial pneumonia in the group of sheep with asbestosis receiving the immunosuppressor drug. We conclude that cyclophosphamide therapy in experimental asbestosis accelerated the fibrotic process and reduced significantly the survival rate of the animals.

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Section: Resultsmentioning

confidence: 83%

Effects of Cyclophosphamide Treatment in Experimental Asbestosis

Bégin

Cantin

Massé

et al. 1988

Experimental Lung Research

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“…This initial macrophage alveolitis may not necessarily lead to interstitial lung fibrosis. Indeed, it has been documented in long-term asbestos and silica workers [53] that the macrophage population of the lower respiratory tract may be expanded in the absence of interstitial lung fibrosing disease; in the animal model, it is a wellrecognized early lung tissue reaction to inert dust particles which usually disappears without residual scar in the months after exposure cessation [54,55]. This type of "transient macrophagic alveolitis" has been observed following latex bead, carbon, graphite, and particulate carborundum exposures (54)(55)(56).…”

Section: Discussionmentioning

confidence: 99%

Chrysotile asbestos exposures can produce an alveolitis with limited fibrosing activity in a subset of high fibre retainer sheep

Bégin

Cantin²,

Sébastien³

1990

Eur Respir J

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Inhalation of fibrogenic mineral dust may enhance fibronectin (Fn) production by alveolar macrophages and increase fibroblast growth activity (FGA) in lung lavage fluid. To investigate the relationship of these changes to fibre retention and the development of asbestosis, we exposed 15 sheep to 100 mg Canadian chrysotile fibres in 100 ml saline, and ten sheep to 100 ml saline only, at ten day intervals. The animals were studied at 3 month intervals. At month 18, ten sheep had abnormal chest radiograph (category greater than or equal to 1) (group B) and five had normal radiograph (category 0) (group A). Pulmonary function data indicated restrictive patterns of abnormalities in both groups, more severe in group B. Sheep in group A had bronchoalveolar lavage (BAL) cellularity and biochemistry comparable to controls; sheep in group B had significant increases in total BAL cells (x2), macrophages (x2), neutrophils (x4) and eosinophils (x3), increased BAL lactate dehydrogenase and Fn, but FGA and procollagen 3 comparable to controls. Fibre retention was significantly increased in all exposed sheep and 2.5 x higher in group B vs group A despite similar exposures (70 intratracheal, 100 mg chrysotile infusions). Enhanced fibre retention in group B preceded the appearance of disease. This study confirms our earlier observation linking individual susceptibility to development of alveolitis to individual dust burden and provides evidence that the excess of fibre retention can be observed before detectable disease. In addition, we report a chrysotile-induced early alveolitis with incompletely expressed fibrosing activity at the time of initial radiographic detection. The intensity of the alveolitis is related to the degree of fibre retention.

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“…Neutrophils are also found in increased numbers in the bronchoalveolar lavage from dust-exposed workers and experimentally exposed animals. [9][10][11][12][13][14][15] On the other hand IARC reviewed in detail the published experimental and epidemiologic studies of cancer in animals and workers exposed to respirable crystalline silica and has concluded that there is sufficient evidence in humans to show carcinogenicity of inhaled crystalline silica in the forms of quartz or cristobalite from occupational sources. [16] In the present study, in order to asses the polymorphonuclear leukocyte functions of foundry and pottery workers occupationally exposed to mainly silica, and other hazardous substances the biomarkers of neutrophil functions i.e., chemotaxis and oxidative burst activity have been evaluated.…”

Section: Introductionmentioning

confidence: 99%

Alterations of Neutrophil Functions in Foundry and Pottery Workers

Başaran

Ündeğer

Shubair³

2002

Immunopharmacology and Immunotoxicology

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To assess the immune competence of workers occupationally exposed to mainly silica, neutrophil functions such as the chemotactic and oxidative burst activity in foundry and pottery workers were evaluated. The chemotactic activity was examined in 22 foundry and 10 pottery workers and oxidative burst activity of neutrophils were determined in 22 foundry and 6 pottery workers. Healthy subjects of comparable age, sex, and smoking habits and with no history of silica exposure were used as the control groups. Chemotaxis was carried out in Boyden chambers using Zymosan activated serum as chemotactic stimulus. Oxidative burst activity was measured using nitroblue tetrazolium (NBT) dye reduction test. Both neutrophil functions were significantly reduced in silica-exposed foundry and pottery workers (p < 0.001) compared to controls suggesting that human chronic exposure mainly to silica and other chemicals originated from foundry and pottery settings may diminish neutrophil functions in humans.

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Assessment of Disease Activity by Gallium-67 Scan and Lung Lavage in the Pneumoconioses

Cited by 34 publications

References 25 publications

Effects of Cyclophosphamide Treatment in Experimental Asbestosis

Effects of Cyclophosphamide Treatment in Experimental Asbestosis

Chrysotile asbestos exposures can produce an alveolitis with limited fibrosing activity in a subset of high fibre retainer sheep

Alterations of Neutrophil Functions in Foundry and Pottery Workers

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