Assessment of direct shoot organogenesis and genetic fidelity in Solanum viarum Dunal—a commercially important medicinal plant

Pandey, Shatrujeet; Patel, Preeti; Prasad, Archana; Sawant, Samir V.; Misra, Pratibha

doi:10.1007/s11627-020-10073-0

Cited by 17 publications

(6 citation statements)

References 43 publications

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“…However, MS medium without PGRs induced no shoot. This is in agreement with Pandey 11 that intermodal explants cultured on MS only did not induce shoot regeneration. At four weeks after culture initiation, the initiated shoot(s) further elongated, and more new shoots developed from the explants (Figure 1).…”

Section: Bap Influenced Direct Organogenesis In Internode Explants Of In Vitro Grown Plantlets Of Solanecio Biafraesupporting

confidence: 93%

“…The result may be due to abrupt modification in the cellular environment, which resulted from the exposure of injured tissues to low nutrient concentrations or PGRs, resulting in stress and subsequently inducing regeneration. 12 Similar reports of successful shoot induction from internode explants on media supplemented with BAP abound in Solanum viarum 11 , Solanum tuberosum. 13 Similar report was obtained in Monochasma savatieri.…”

Section: Bap Influenced Direct Organogenesis In Internode Explants Of In Vitro Grown Plantlets Of Solanecio Biafraementioning

confidence: 74%

“…Previous reports revealed that shoots were successfully induced from petiole explants with BA in Solanum viarum Dunal. 11 However, shoots were formed directly in the leaf explants of Chrysanthemum morifolium on the media supplemented with BAP. 18 In contrast, leaf segments of S. viarum did not induce any shoot with BA.…”

Section: Bap Alone or In Combination With Iba Influenced Direct Organogenesis In Leaf Lamina Leaf With Petiole And Petiole Explants Of Inmentioning

confidence: 99%

“…18 In contrast, leaf segments of S. viarum did not induce any shoot with BA. 11 Adventitious shoots were also obtained in Titanotrichum oldhamii using medium supplemented with 0.1mg/L BA. 19 Meanwhile, in Bacopa monnieri, BAP (1.5 mg/L) in combination with IAA (0.5 mg/L) induced 220 multiple shoots regenerated from leaf explants.…”

Section: Bap Alone or In Combination With Iba Influenced Direct Organogenesis In Leaf Lamina Leaf With Petiole And Petiole Explants Of Inmentioning

confidence: 99%

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In vitro Regeneration of Solanecio biafrae Through Direct Shoot Organogenesis

2021

TJNPR

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Solanecio biafrae (Olive & Hierne) C. Jeffrey (Asteraceae) is a tropical, underutilized African indigenous vegetable, highly nutritious and medicinal. It grows as undercover and is mostly collected in tree plantations such as cocoa and kola. Its propagation is seldom carried out by vines, but its climbing habit poses a problem for farmers, which leads to the application of herbicide to control their spread. In order to ascertain its potential for genetic improvement, the protocol for direct shoot organogenesis of S. biafrae was developed. Leaf lamina, petiole, and leaf with petiole explants were cultured on Murashige and Skoog (MS) medium supplemented with varying concentrations of 6-benzylaminopurine (BAP) alone and in combination with Indole butyric acid (IBA). Direct shoot organogenesis was obtained from the cut end of leaf lamina and petiole explants as well from the petiolar region of the leaf with petiole explant. The results obtained from this study show the potential of these explants of S. biafrae to serve as culture material for its micropropagation, genetic improvement via transgenesis. Hence, this regeneration protocol of S. biafrae can be coupled to its genetic transformation to improve species with shorter internode and more leaves to enhance the structure of tropical vegetation.

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Section: Bap Influenced Direct Organogenesis In Internode Explants Of In Vitro Grown Plantlets Of Solanecio Biafraesupporting

confidence: 93%

Section: Bap Influenced Direct Organogenesis In Internode Explants Of In Vitro Grown Plantlets Of Solanecio Biafraementioning

confidence: 74%

Section: Bap Alone or In Combination With Iba Influenced Direct Organogenesis In Leaf Lamina Leaf With Petiole And Petiole Explants Of Inmentioning

confidence: 99%

Section: Bap Alone or In Combination With Iba Influenced Direct Organogenesis In Leaf Lamina Leaf With Petiole And Petiole Explants Of Inmentioning

confidence: 99%

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In vitro Regeneration of Solanecio biafrae Through Direct Shoot Organogenesis

2021

TJNPR

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“…A higher regeneration rate (> 30 shoots per explants) was noted for root explants (Turker et al 2008;Dreger et al 2016), but without respect to the genotype effect. Type of explant and PGRs play a key role in the regeneration process, but its efficiency depends on genotype (Zimik and Arumugam 2017;Pandey et al 2020). Genotypes differ in their levels of endogenous phytohormones.…”

Section: Discussionmentioning

confidence: 99%

Phytochemical variability during vegetation of Chamerion angustifolium (L.) Holub genotypes derived from in vitro cultures

Dreger

Seidler-Łożykowska

Szalata

et al. 2021

Plant Cell Tiss Organ Cult

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The purpose of the study was to evaluate Chamerion angustifolium (L.) Holub genotypes for preliminary selection and further breeding programs aimed at obtaining a suitable industrial form for the pharmaceutical applications. Clonally propagated plants representing 10 genotypes of Ch. angustifolium were regenerated under in vitro conditions, hardened and planted in the field. Studies included an evaluation of shoot proliferation, phytochemical assessment of in vitro and ex vitro plants as well as investigations of intraspecies variability regarding four phenological stages: vegetative, beginning of blooming, full blooming, and green fruit phases. Quantitative and qualitative analyses of bioactive compounds were performed using high-performance liquid chromatography coupled with diode array detector and tandem mass spectrometer (HPLC–DAD–MS/MS) and high-performance liquid chromatography (HPLC) methods. The efficiency of shoot multiplication varied between genotypes from 8.12 to 21.48 shoots per explant. A high reproduction rate (> 20 shoots per explant) was recorded for four lines (PL_45, PL_44, PL_58, DE_2). Plants grown in vitro synthesized oenothein B (11.2–22.3 mg g−1 DW) and caffeic acid derivatives. Plants harvested from field contained the full spectrum of polyphenols characteristic for this species, and oenothein B and quercetin 3-O-glucuronide were the most abundant. The maximal content of oenothein B was determined in the vegetative phase of fireweed, while some flavonoids were found in the highest amount in full blooming phase. The results of analysis of variance indicated significant differences among genotypes in oenothein B, 3-O-caffeoylquinic acid and flavonoids accumulation in four phenological phases. PL_44 plants were characterized by high content of oenothein B and quercetin 3-O-glucuronide as well as a relatively high level of other flavonoids. Based on our phytochemical and micropropagation studies, PL_44 genotype was the best candidate for early selection and further breeding programs.

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