Assessment of Different Bariatric Surgeries in the Treatment of Obesity and Insulin Resistance in Mice

Yin, Deng Ping; Gao, Qiang; Li, Lian; Yan, Wen; Williams, Phillip E.; McGuinness, Owen P.; Wasserman, David H.; Abumrad, Naji N.

doi:10.1097/sla.0b013e3182197035

Cited by 53 publications

(55 citation statements)

References 47 publications

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“…In our work, circulating insulin was significantly increased in DIO mice, but it was reduced by BD, which is consistent with our previous finding that the RYGB procedure improves ␤-cell viability (61). Leptin, secreted by adipocytes, is linked to obesity, insulin resistance, and inflammatory responses.…”

Section: Discussionsupporting

confidence: 92%

Activation of bile acid signaling improves metabolic phenotypes in high-fat diet-induced obese mice

Pierre

Martinez

et al. 2016

American Journal of Physiology-Gastrointestinal and Liver Physi

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EB, Yin DP. Activation of bile acid signaling improves metabolic phenotypes in high-fat diet-induced obese mice. Am J Physiol Gastrointest Liver Physiol 311: G286 -G304, 2016. First published June 23, 2016 doi:10.1152/ajpgi.00202.2016.-The metabolic benefits induced by gastric bypass, currently the most effective treatment for morbid obesity, are associated with bile acid (BA) delivery to the distal intestine. However, mechanistic insights into BA signaling in the mediation of metabolic benefits remain an area of study. The bile diversion () mouse model, in which the gallbladder is anastomosed to the distal jejunum, was used to test the specific role of BA in the regulation of glucose and lipid homeostasis. Metabolic phenotype, including body weight and composition, glucose tolerance, energy expenditure, thermogenesis genes, total BA and BA composition in the circulation and portal vein, and gut microbiota were examined. BD improves the metabolic phenotype, which is in accord with increased circulating primary BAs and regulation of enterohormones. BD-induced hypertrophy of the proximal intestine in the absence of BA was reversed by BA oral gavage, but without influencing BD metabolic benefits. BD-enhanced energy expenditure was associated with elevated TGR5, D2, and thermogenic genes, including UCP1, PRDM16, PGC-1␣, PGC-1␤, and PDGFR␣ in epididymal white adipose tissue (WAT) and inguinal WAT, but not in brown adipose tissue. BD resulted in an altered gut microbiota profile (i.e., Firmicutes bacteria were decreased, Bacteroidetes were increased, and Akkermansia was positively correlated with higher levels of circulating primary BAs). Our study demonstrates that enhancement of BA signaling regulates glucose and lipid homeostasis, promotes thermogenesis, and modulates the gut microbiota, which collectively resulted in an improved metabolic phenotype. bile acids; bile diversion; obesity; energy expenditure; gut microbiota; gastric bypass; mice

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Section: Discussionsupporting

confidence: 92%

Activation of bile acid signaling improves metabolic phenotypes in high-fat diet-induced obese mice

Pierre

Martinez

et al. 2016

American Journal of Physiology-Gastrointestinal and Liver Physi

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“…Similarly, glucose tolerance in OAGB mice improved significantly and was comparable to that at the baseline evaluation. These results match experiences from other murine models of bariatric surgery [15, 19]. Chambers et al [20] observed similar effects on glucose tolerance in a mouse model of RYGB and sleeve gastrectomy.…”

Section: Discussionsupporting

confidence: 82%

“…Weight loss was rapid during the first week and continued moderately. Other authors observed similar effects on body weight after RYGB [15, 16]. Sustained weight loss after RYGB depends on the experimental diet and preoperative weight and ranges between 20 and 30% in the literature [16, 17].…”

Section: Discussionmentioning

confidence: 73%

A New Physiologic Mouse Model of One Anastomosis Gastric Bypass

et al. 2018

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Background: One anastomosis gastric bypass (OAGB) is a modern metabolic operation that has been demonstrated to be a rapid, safe, and effective procedure. As for other bariatric operations, the mechanisms and long-term effects of this procedure remain largely unknown and are difficult to address in human studies. Here, we present a new physiologic mouse model for mechanistic and long-term investigations. Methods: Six-week-old C57Bl/6 mice were fed a high-fat diet for 12 weeks and scheduled for OAGB or sham operation. Mice were observed for 2 weeks after the operation, and weight and metabolic condition were monitored. Results: Six mice were used to adapt the surgical technique. Afterwards, another 7 mice were scheduled for OAGB without further complications. The newly established OAGB procedure resulted in significant weight loss and improvement of glucose metabolism 2 weeks after the operation. Conclusions: The operation presented here is an easy-to-learn and physiologic mouse model of OAGB that can be used for further studies in mice.

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“…During the experiment, 1 rat from the sham group, 1 rat from the RYGBP group and 2 rats from the RYGBP+IL group succumbed. This is in line with the mortality rates of rats in previous studies (25)(26)(27).…”

Section: Animalssupporting

confidence: 92%

Effect of gastric bypass combined with ileal transportation on type 2 diabetes mellitus

et al. 2018

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Abstract. Type 2 diabetes mellitus (T2DM) is a chronic progressive disease, which manifests as an endocrine disorder. Among the different methods of surgery available to treat patients with T2DM, Roux-en-Y gastric bypass (RYGBP) and ileal transposition (IT) are the most commonly performed. The aim of the present study was to investigate the effects of RYGBP combined with IT on rats with T2DM. A total of 8 healthy male rats were used as a control group and 40 GK rats were randomly divided into 5 groups: A diabetes mellitus (DM) group, a sham operative group (SO), a RYGBP group, an IT group and a RYGBP+IT group. The results demonstrated that fasting blood glucose, triglyceride, total cholesterol and gastric inhibitory polypeptide levels in all treatment groups were significantly lower than those of the SO and DM groups. Furthermore, levels TC and TG in the RYGBP+IT group were significantly lower than in the RYGBP and IT groups. Levels of phosphoenolpyruvate carboxykinase and glucose-6-phosphatase mRNA and IRS-2 protein in all treatment groups were also significantly lower than those of the SO group; and they were significantly lower in the RYGBP+IT group compared with the RYGBP and IT groups. The expression of phosphorylated Akt in the treatment groups was significantly higher than the SO group and was significantly higher in the RYGBP+IT group compared with the RYGBP and IT groups. These results indicate that RYGBP and IT surgical treatment can induce T2DM remission by mediating the expression of insulin-related factors to reverse insulin resistance. The current study also indicated that the effect of RYGBP combined with IT may be developed as a novel first-line method of treating T2DM.

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Assessment of Different Bariatric Surgeries in the Treatment of Obesity and Insulin Resistance in Mice

Cited by 53 publications

References 47 publications

Activation of bile acid signaling improves metabolic phenotypes in high-fat diet-induced obese mice

Activation of bile acid signaling improves metabolic phenotypes in high-fat diet-induced obese mice

A New Physiologic Mouse Model of One Anastomosis Gastric Bypass

Effect of gastric bypass combined with ileal transportation on type 2 diabetes mellitus

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