Assessment of dietary lipid sources in tropical gar,Atractosteus tropicuslarvae: Growth parameters and intermediary lipogenic gene expression

Abstract

Five experimental diets containing different lipid sources, fish oil (D1), soybean lecithin (D2), corn oil (D3), canola oil (D4) and olive oil (D5), were evaluated in Atractosteus tropicus larvae for the relative gene expression of the enzymes fatty acid synthase (fas), acetyl‐CoA carboxylase 1 (acc1) and carnitine palmitoyltransferase 1C (cpt1c), in addition to their effects on larval growth, survival and cannibalism during a 30‐day feeding trial. Higher growth and survival were obtained in treatments D1 and D2, and lower performance in diets D3, D4 and D5. The highest levels of expression of fas and acc1 occurred in larvae fed with D1, which contained high amounts of n‐3 long‐chain polyunsaturated fatty acids (LC‐PUFA), mainly DHA and EPA FA are regulators of lipogenesis. The higher cpt1c expression in plant‐based diets is attributed to the fact that these diets are rich in α‐linolenic acid (ALA) and low DHA, EPA and ARA levels that favour ß‐oxidation. In conclusion, the diets with fish oil (D1) and soybean lecithin (D2) were the best treatments for larval growth, survival and cannibalism and thus appear to meet both lipid and energy requirements of A. tropicus larvae, meanwhile the use of vegetable oils influences the expression of intermediary lipogenic genes.

Modelling the potential distribution of two tropical freshwater fish species under climate change scenarios

“…Despite the ecological and conservation importance of these two predators, information about their biology and ecology is scarce; the available information deals primarily with aquaculture and nutritional aspects (Jiménez‐Martínez et al, 2020; Rodríguez‐Estrada et al, 2020). The lack of information about their natural populations prevents establishing sound present and future conservation strategies.…”

“…the available information deals primarily with aquaculture and nutritional aspects (Jiménez-Martínez et al, 2020;Rodríguez-Estrada et al, 2020). The lack of information about their natural populations prevents establishing sound present and future conservation strategies.…”

Expression of long-chain polyunsaturated fatty acids biosynthesis genes during the early life-cycle stages of the tropical gar Atractosteus tropicus

“…Progresses have been made to understand important aspects of the A. tropicus nutrition and physiology, those include the characterization of digestive enzyme activities and the histological description of the digestive system through the species early development (Frías-Quintana et al, 2015), estimation of dietary lipid requirements (Huerta-Ortíz et al, 2018), the relative gene expression of fatty acid synthase (fas), acetyl-CoA carboxylase 1 (acc1) and carnitine palmitoyltransferase 1C (cpt1c) during larval ontogeny and across juvenile's organs (Jiménez-Martínez et al, 2019), as well as the effect of dietary lipid sources on growth, survival, cannibalism, and the relative gene expression fas, acc1 and cpt1c during early development (Jiménez-Martínez et al, 2020). However, little is known about the ability of A. tropicus to efficiently utilize vegetal oils-based diets that are more sustainable, which could help to reduce the feed production cost.…”