Assessment of Dental Pulp Stem Cell (DPSC) Biomarkers Following Induction with Bone Morphogenic Protein 2 (BMP-2)

Cinelli, Joseph; Nguyen, Eileen; Kingsley, Karl

doi:10.9734/jabb/2018/44215

Cited by 5 publications

(3 citation statements)

References 38 publications

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“…Growth factors were obtained from Fisher Scientific, which included vascular endothelial growth factor (VEGF; catalog PHC9393) and bone morphogenic protein (BMP; catalog PHC7141). Experimental wells were treated with growth factor (VEGF or BMP-2) at physiologically relevant concentrations approximating 10 ng/mL—within the range of other studies of DPSC responsiveness to these growth factors [ 27 , 28 , 29 , 30 ]. Mineralized trioxide aggregate (MTA) was obtained from Henry Schein (catalog 7040069).…”

Section: Methodsmentioning

confidence: 99%

Characterization of Dental Pulp Stem Cell Responses to Functional Biomaterials Including Mineralized Trioxide Aggregates

Bae

Kang

Lee

et al. 2021

JFB

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Introduction: Many studies in stem cell biology have demonstrated that dental pulp stem cells (DPSC) may be highly proliferative and capable of pluripotent differentiation into many different tissue types. Recent advances in stem cell research have outlined methods for directing in vitro or in vivo growth, viability, and proliferation, as well as differentiation of DPSC—although much remains to be discovered. Based upon this information, the primary objective of this study was to understand the functional biomaterials needed to more effectively direct DPSC viability, growth, and proliferation. Methods: Using an approved protocol, previously collected and isolated samples of DPSC from an existing repository were used. Previously established stem cell biomarkers (Sox-2, Oct-4, NANOG) from each isolate were correlated with their proliferation rates or doubling times to categorize them into rapid, intermediate, or slow-dividing multipotent DPSC. Growth factors and other functional dental biomaterials were subsequently tested to evaluate DPSC responses in proliferation, viability, and morphology. Results: Differential responses were observed among DPSC isolates to growth factors, including vascular endothelial growth factor (VEGF) and bone morphogenic protein (BMP-2), and functional biomaterials such as mineralized trioxide aggregates (MTA). The responsiveness of DPSC isolates did not correlate with any single factor but rather with a combination of proliferation rate and biomarker expression. Conclusions: These data strongly suggest that some, but not all, DPSC isolates are capable of a robust and significant in vitro response to differentiation stimuli, although this response is not universal. Although some biomarkers and phenotypes that distinguish and characterize these DPSC isolates may facilitate the ability to predict growth, viability, and differentiation potential, more research is needed to determine the other intrinsic and extrinsic factors that may contribute to and modulate these DPSC responses to these functional biomaterials for biotechnology and bioengineering applications.

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Section: Methodsmentioning

confidence: 99%

Characterization of Dental Pulp Stem Cell Responses to Functional Biomaterials Including Mineralized Trioxide Aggregates

Bae

Kang

Lee

et al. 2021

JFB

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“…These data provided both live and total cell counts, allowing for the calculation of the percentage of viable cells in each sample, as previously described. [27,28,31] Digital images of DPSC isolates were captured using the Lionheart LX Automated Microscope and ×20 objective lens.…”

Section: Human Subjectsmentioning

confidence: 99%

Pluripotent Stem Cell Markers and microRNA Expression May Correlate with Dental Pulp Stem Cell Viability and Proliferation Rates

Whiting,

J.Hatton,

Kingsley

2019

Ascl Med Res Rev

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Background: Recent evidence has demonstrated that dental pulp stem cells (DPSC) may represent a source of pluripotent progenitors capable of differentiating into many cell and tissue types. Although microRNAs are known to modulate differentiation and function in human dental tissues, much of this research has focused selectively on tooth development. The primary objective of this study was to evaluate the expression of microRNA in dental pulp stem cell isolates to compare with classical biomarkers of cellular phenotypes and pluripotency. Materials and Methods: Using eight previously isolated and characterized DPSC isolates, growth and viability were evaluated and RNA was extracted for mRNA screening. DPSC biomarker and microRNA expression were analyzed for comparison with cellular phenotypes. Results: Evaluation of the growth and proliferation rates of each cell line resulted in the categorization of DPSC isolates into rapid, intermediate, and slow doubling times, which demonstrated higher viability among the most rapidly proliferating DPSCs. Analysis of DPSC biomarkers (Oct-4, Sox-2, NANOG) revealed an association with total live cell count, while microRNA expression (miR-27, miR-218, miR-124, and miR-16) appeared to be more closely associated with cellular viability. Conclusions: Although this study was limited to a small number of DPSC isolates, these results suggest a more thorough investigation and evaluation of biomarkers and microRNA expression may be necessary to elucidate the associations and complex interconnections with DPSC viability, proliferation, differentiation, and pluripotency.

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“…However, most of these efforts have focused on the potential for DPSCs towards dentinogenesis and osteogenesis [ 20 , 21 , 22 ]. In fact, many studies from this group have also explored the potential to modulate DPSC phenotypes using BMP and VEGF [ 23 , 24 , 25 , 26 ].…”

Section: Introductionmentioning

confidence: 99%

Differential Effects of Extracellular Matrix Glycoproteins Fibronectin and Laminin-5 on Dental Pulp Stem Cell Phenotypes and Responsiveness

Lee

Bae

Kim

et al. 2023

JFB

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Dental pulp stem cells (DPSCs) are mesenchymal stem cells (MSCs) with the potential to differentiate in a limited number of other tissue types. Some evidence has suggested the modulation of DPSC growth may be mediated, in part, by exogenous extracellular matrix (ECM) glycoproteins, including fibronectin (FN) and laminin-5 (LN5). Although preliminary research suggests that some ECM glycoproteins may work as functional biomaterials to modulate DPSC growth responses, the primary goal of this project is to determine the specific effects of FN and LN5 on DPSC growth and viability. Using an existing DPSC repository, n = 16 DPSC isolates were cultured and 96-well growth assays were performed, which revealed FN, LN5 and the combination of these were sufficient to induce statistically significant changes in growth among five (n = 5) DPSC isolates. In addition, the administration of FN (either alone or in combination) was sufficient to induce the expression of alkaline phosphatase (ALP) and dentin sialophosphoprotein (DSPP), while LN5 induced the expression of ALP only, suggesting differential responsiveness among DPSCs. Moreover, these responses appeared to correlate with the expression of MSC biomarkers NANOG, Oct4 and Sox2. These results add to the growing body of evidence suggesting that functional biomaterials, such as ECM glycoproteins FN and LN5, are sufficient to induce phenotypic and differentiation-specific effects in a specific subset of DPSC isolates. More research will be needed to determine which biomarkers or additional factors are necessary and sufficient to induce the differentiation and development of DPSCs ex vivo and in vitro for biomedical applications.

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Assessment of Dental Pulp Stem Cell (DPSC) Biomarkers Following Induction with Bone Morphogenic Protein 2 (BMP-2)

Cited by 5 publications

References 38 publications

Characterization of Dental Pulp Stem Cell Responses to Functional Biomaterials Including Mineralized Trioxide Aggregates

Characterization of Dental Pulp Stem Cell Responses to Functional Biomaterials Including Mineralized Trioxide Aggregates

Pluripotent Stem Cell Markers and microRNA Expression May Correlate with Dental Pulp Stem Cell Viability and Proliferation Rates

Differential Effects of Extracellular Matrix Glycoproteins Fibronectin and Laminin-5 on Dental Pulp Stem Cell Phenotypes and Responsiveness

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