Assessment of decellularization of heart bioimplants using a Raman spectroscopy method

Abstract

We report the results of experimental studies on cardiac implants using a Raman spectroscopy method (RS). Raman spectra characteristics of leaves and walls of cardiac implants were obtained; the implants were manufactured by protocols of detergent-enzymatic technique (DET) and biological, detergent-free (BIO) decellularization, using detergents (group DET) or a detergent-free, nonproteolytic, actin-disassembling regimen (BIO). There were input optical coefficients that allowed us to carry out evaluation of the protocols of DET and BIO decellularization on the basis of the concentrations of glycosaminoglycans, proteins, amides, and DNA. It was shown that during DET and BIO decellularization, composition aberrations of proteins and lipids do not occur and the integrity of the collagenous structures is preserved. It was found that during the DET decellularization, preservation of glycosaminoglycans is better than during BIO decellularization.

Structural assessments in decellularized extracellular matrix of porcine semilunar heart valves: Evaluation of cell niches

“…However, when we analyzed with a chemometric tool to provide the information of spectra variations, a worthy evidence was revealed. The PCA applied in spectra between 600 and 1800 cm −1 , fingerprint region of biological samples, demonstrated that the main differences observed were in the bands at 1096 and 1340 cm −1 , which are associated with nucleic acids/phospholipids and protein deformation, also observed by Timchenko et al in an analysis of the decellularization effect on cardiac implants. The main differences in structures were explained by PC1, corresponding to aortic leaflets (54.34%), pulmonary leaflets (77.65%), aortic conduit (73.41%), and pulmonary conduit (60.42%).…”

A non-linear mathematical model using optical sensor to predict heart decellularization efficacy

“…These findings open up new possibilities for the use of diversified types of optical data in order to standardize extracellular matrices after decellularization, and these data may be used alongside our optical system. Raman Scattering Spectroscopy– RS 37 is another nondestructive alternative for tissue engineering, and can identify changes in signal intensity that indicate changes in the composition and proportions of matrix components in cardiac tissues. At its current stage of development, research in RS is still focused on tissue evaluation, but it has demonstrated potential for application to whole organ decellularization in the future.…”

Спектральный Анализ Органических Компонентов Деминерализованных Костных Биоимплантатов-=sup=-*-=/Sup=-

“…Коллаген, гликозаминогликаны, протеогликаны, тирозин, пролин и фенилаланин являются основными компонентами экстраклеточного матрикса. Качество имплантатов определяется его сохранностью и полнотой удаления клеточных компонентов донора (ДНК, РНК) [7,8].…”

“…Поэтому необходим постоянный контроль качества изготовляемых имплантатов с оценкой органической составляющей. На современном уровне развития науки и техники для решения этой задачи могут быть применены оптические методы исследования, поскольку они могут использоваться как скрининговые, быстро выполняемые, малозатратные методы, без разрушения представленных образцов в отличие от морфологических, морфометрических, биохимических исследований [7,8]. Метод спектроскопии комбинационного рассеяния (КР) [9][10][11] находит широкое применение для контроля качества материалов тканевой инженерии.…”

“…модулем LuxxMaster LML-785.0RB-04 (мощность до 500 mW, длина волны 784.7 ± 0.05 nm), и высокоразрешающий спектрометр Shamrock sr-303i, обеспечивающий спектральное разрешение 0.15 nm, со встроенной охлаждаемой камерой DV420A-OE[7,8].Качественный анализ белков проведен на хроматографе Dionex Ultimate 3000 с использованием колонки AcclaimPepMap C18, 2 µm, 100 ¦ , 75 µm × 15 cm…”