Assessment of dead-end ultrafiltration for the detection and quantification of microbial indicators and pathogens in the drinking water treatment processes

Pascual-Benito, Míriam; Emiliano, Pere; Casas-Mangas, Raquel; Dacal-Rodríguez, Cristina; Gracenea, Mercedes; Araujo, Rosa; Valero, Fernando; García-Aljaro, Cristina; Lucena, F.

doi:10.1016/j.ijheh.2020.113628

Cited by 7 publications

(5 citation statements)

References 55 publications

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“…Historical data from this DWTP (from 2011 to 2019, data not shown), based on the use of standard membrane filtration for routine water quality monitoring, indicate positive results for these indicators only at early stages (i.e., E. coli and somatic coliphages until sand filtration, or intestinal enterococci until carbon filtration). Previous studies using the DEUF method have reported recoveries of 45.5 ± 24.0 % for fecal indicators and 22.4 ± 9.3% for bacteriophages [47], ranging from 35 to 95% for enteric bacteria and viruses [48] or 60-80% for bacteria, viruses and protozoa in drinking water [20], depending on the water matrix and degree of turbidity. Therefore, the reported concentrations for the different indicators may be even higher than in the current study.…”

Section: Discussionmentioning

confidence: 98%

Monitoring Bacterial Community Dynamics in a Drinking Water Treatment Plant: An Integrative Approach Using Metabarcoding and Microbial Indicators in Large Water Volumes

Pinar-Méndez

Wangensteen

Præbel

et al. 2022

Water

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Monitoring bacterial communities in a drinking water treatment plant (DWTP) may help to understand their regular operations. Bacterial community dynamics in an advanced full-scale DWTP were analyzed by 16S rRNA metabarcoding, and microbial water quality indicators were determined at nine different stages of potabilization: river water and groundwater intake, decantation, sand filtration, ozonization, carbon filtration, reverse osmosis, mixing chamber and post-chlorination drinking water. The microbial content of large water volumes (up to 1100 L) was concentrated by hollow fiber ultrafiltration. Around 10 million reads were obtained and grouped into 10,039 amplicon sequence variants. Metabarcoding analysis showed high bacterial diversity at all treatment stages and above all in groundwater intake, followed by carbon filtration and mixing chamber samples. Shifts in bacterial communities occurred downstream of ozonization, carbon filtration, and, more drastically, chlorination. Proteobacteria and Bacteroidota predominated in river water and throughout the process, but in the final drinking water, the strong selective pressure of chlorination reduced diversity and was clearly dominated by Cyanobacteria. Significant seasonal variation in species distribution was observed in decantation and carbon filtration samples. Some amplicon sequence variants related to potentially pathogenic genera were found in the DWTP. However, they were either not detected in the final water or in very low abundance (<2%), and all EU Directive quality standards were fully met. A combination of culture and high-throughput sequencing techniques may help DWTP managers to detect shifts in microbiome, allowing for a more in-depth assessment of operational performance.

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Section: Discussionmentioning

confidence: 98%

Monitoring Bacterial Community Dynamics in a Drinking Water Treatment Plant: An Integrative Approach Using Metabarcoding and Microbial Indicators in Large Water Volumes

Pinar-Méndez

Wangensteen

Præbel

et al. 2022

Water

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“…These results corroborate the spatial and temporal shift in the bacterial populations. Bacteria play indispensable roles in various biogeochemical processes and pollutant degradation, and they can be useful in assessing the health of the aquatic ecological environment [89,90]. We did not analyze the impact of nutrient pollution on the bacterial communities, but there was a gradient in the nutrient content (PO 4 3− and NH 4 + ) from the most polluted sites to the less polluted sites.…”

Section: Bacterial Communities' Taxonomymentioning

confidence: 99%

Spatio-Temporal Dynamics of crAssphage and Bacterial Communities in an Algerian Watershed Impacted by Fecal Pollution

Boulainine,

Benhamrouche,

Ballesté

et al. 2024

Water

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This study investigates the influence of urban pollution and climate dynamics on water quality and the bacterial communities in an Argelian watershed. Twenty-one sampling campaigns were conducted over two years at six sites along the Oued Boussellam, a river impacted by the effluent of a sewage treatment plant, from a low-polluted site to a water reservoir within a 50 km distance. Fecal indicators and the human fecal marker crAssphage were monitored. Illumina 16S rRNA amplicon sequencing was used to assess water microbial populations’ changes. Urban sewage discharge had an impact on the river quality and microbial ecosystem, which was attenuated along the river course. Significant reductions (>4 log10 for E. coli and somatic coliphages, >3 log10 for crAssphage) occurred, particularly during high-temperature periods. crAssphage correlated strongly with somatic coliphages downstream the river. Seasonal differences were observed in the diversity of the bacterial communities, with higher values during the high-temperature period. The genus-level community structure was similar at highly polluted river sites, also displaying seasonal differences. Despite high pollution levels, natural processes reduced fecal indicators to acceptable levels in the reservoir as well as shaped the bacterial communities along the river, highlighting the importance of understanding indicator persistence and microbial community resilience for effective water quality management within the context of the global warming scenario.

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“…Some bacteria transition into a non-culturable state [66], and therefore, these would not have been detected by the microbiological methods used in this study. To visualise the full scope of bacteria present, other microbiological methods could be useful, e.g., ultrafiltration of a larger amount of water that flows through the drinking equipment and analysing the sediments with PCR [67][68][69]. To visualise the actual biofilm, an electron microscopic scan could be used [70].…”

Section: Biofilm Formationmentioning

confidence: 99%

Drinking Pipes and Nipple Drinkers in Pig Abattoir Lairage Pens—A Source of Zoonotic Pathogens as a Hazard to Meat Safety

Buder,

Meemken,

Fürstenberg

et al. 2023

Microorganisms

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The water distribution system in the lairage pens of abattoirs could act as a route of contamination for produced meat. In this study, biofilm formation and the occurrence of specific pathogens in drinking equipment was investigated in different lairage pens in a German commercial pig abattoir. Samples of the water and the drinkers in different locations were microbiologically cultivated and examined. After new drinking equipment had been installed for one month, three months and five years, biofilm formation was detectable, and retrograde growth from the nipple drinkers was seen up to the connection with the main water distribution system. In particular, Enterobacteriaceae and Pseudomonas spp. were found in all samplings of the nipple drinkers. Zoonotic pathogens, Salmonella, pathogenic Yersinia enterocolitica and methicillin-resistant Staphylococcus aureus, were also isolated from the nipple drinkers, while Listeria monocytogenes was not detected via microbial cultivation methods in any of the samples. Since the pigs take the contaminated nipple drinkers into their mouths to drink, or drink contaminated water containing the pathogens, transmission and even infection of the pigs in the lairage can be assumed. This could consequently lead to contamination or cross-contamination of the meat during slaughter and processing and to a public health risk.

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Assessment of dead-end ultrafiltration for the detection and quantification of microbial indicators and pathogens in the drinking water treatment processes

Cited by 7 publications

References 55 publications

Monitoring Bacterial Community Dynamics in a Drinking Water Treatment Plant: An Integrative Approach Using Metabarcoding and Microbial Indicators in Large Water Volumes

Monitoring Bacterial Community Dynamics in a Drinking Water Treatment Plant: An Integrative Approach Using Metabarcoding and Microbial Indicators in Large Water Volumes

Spatio-Temporal Dynamics of crAssphage and Bacterial Communities in an Algerian Watershed Impacted by Fecal Pollution

Drinking Pipes and Nipple Drinkers in Pig Abattoir Lairage Pens—A Source of Zoonotic Pathogens as a Hazard to Meat Safety

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