Assessment of day-3 morphology and euploidy for individual chromosomes in embryos that develop to the blastocyst stage

Eaton, Jennifer L.; Hacker, Michele R.; Harris, Doria; Thornton, Kim L.; Penzias, Alan S.

doi:10.1016/j.fertnstert.2008.03.008

Cited by 45 publications

(38 citation statements)

References 40 publications

(35 reference statements)

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“…Patients underwent treatment protocols for ovarian stimulation, monitoring, and oocyte retrieval as previously described [7]. The number of embryos transferred into the uterus was consistent with national guidelines [8] and decreased in the <35 age groups from a mean of approximately 2.5 to 1.7 during the study period (unpublished data).…”

Section: Methodsmentioning

confidence: 91%

“…The number of embryos transferred into the uterus was consistent with national guidelines [8] and decreased in the <35 age groups from a mean of approximately 2.5 to 1.7 during the study period (unpublished data). Patients received luteal phase support until 8 weeks of gestation [7]. Intracytoplasmic sperm injection (ICSI) and assisted hatching were performed when indicated.…”

Section: Methodsmentioning

confidence: 99%

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In vitro fertilization in women under 35: counseling should differ by age

Humm

Dodge

et al. 2015

J Assist Reprod Genet

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Purpose The aim of this study is to evaluate the outcomes of in vitro fertilization (IVF), including cumulative live birth rate, among women <25 years, 25 to <30 years, and 30 to <35 years. Methods A retrospective cohort study of all women 18 to <35 years of age at their first fresh-embryo, non-donor IVF cycle from January 1995 through December 2012 at a single center was conducted. A competing-risk regression model was used to estimate the cumulative probability and 95 % confidence interval (CI) of the first live birth in up to 6 cycles during the study period with IVF cycle number as the time metric. Results Among 7243 women who underwent 16,792 cycles, there were 163 (2.3 %) women <25 years, 1691 (23.3 %) women 25 to <30 years, and 5389 (74.4 %) women 30 to <35 years. Women <25 years had the lowest cumulative live birth rate after each cycle, followed by women 30 to <35 years. In both groups, the cumulative live birth rate after 6 cycles was significantly lower than that of women 25 to <30 years; these rates were 58 % (95 % CI 0.51-0.66) among women <25 years, 69 % (95 % CI 0.67-0.71) among women 25 to <30 years, and 64 % (95 % CI 0.63-0.65) among women 30 to <35 years. Conclusions Our findings are consistent with other reports of less favorable IVF treatment outcomes in women <25 years of age following their first IVF cycle. This indicates that there are underlying factors in couples with a female <25 years of age that should lead to different treatment counseling when they attempt IVF.

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Section: Methodsmentioning

confidence: 91%

Section: Methodsmentioning

confidence: 99%

In vitro fertilization in women under 35: counseling should differ by age

Humm

Dodge

et al. 2015

J Assist Reprod Genet

View full text Add to dashboard Cite

show abstract

“…Although preliminary, these studies have shown a weak association between aneuploidy and abnormal embryo morphology. (2,(5)(6)(7)(11)(12)(13). Alfarawati et al (2) showed that aneuploidy negatively affected the ICM and TE grades.…”

Section: Discussionmentioning

confidence: 99%

Blastocyst morphology holds clues concerning the chromosomal status of the embryo

Figueira

Setti

Braga

et al. 2012

Fertility and Sterility

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“…Patients underwent ovarian stimulation protocols with gonadotropins and either a GnRH agonist or antagonist as previously described [33]. Cycles were monitored with daily serum estradiol levels and transvaginal ultrasound examinations beginning on treatment days 6 to 8.…”

Section: Protocolsmentioning

confidence: 99%

Clustering of monozygotic twinning in IVF

Vaughan

Ruthazer

Penzias

et al. 2015

J Assist Reprod Genet

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Purpose The aim of this analysis was to study whether monozygotic twinning (MZT) events occur in clusters after IVF and, if so, to explore possible explanations for this clustering. Methods This is a retrospective cohort study carried out in a single, large university-affiliated reproductive medicine practice. Medical records of all patients who had undergone fresh IVF cycles, resulting in a viable clinical pregnancy, from Jan 2002 to Dec 2013 were reviewed. The incidence of MZT in 6-month intervals and the association with independent risk factors such as maternal age, extended embryo culture, PGD/intracytoplasmic sperm injection (ICSI)/assisted hatching performed were analyzed. Results Over the 12-year study period, 25,502 fresh IVF cycles were performed, resulting in 8598 clinical pregnancies.Ninety-five cycles (1.1 %) resulted in MZ twins. The percentage of MZT was >2 standard deviations (SD) higher than the overall percentage of MZT in 4 of the 24 6-month intervals. PGD, extended embryo culture (≥4 days), and more recent cycles (2005 or later) were independent risk factors for MZT. The use of multivariable logistic regression modeling to control for risk factors for MZT did not correct for this clustering effect, with both high-risk interval (clustering) and extended embryo culture remaining significant. Conclusion This study supports our hypothesis that MZT occurs in clusters and that this clustering effect could not be explained by demographics and cycle characteristics alone. Although we are unable to explain the clustering phenomenon, this study is important as it highlights high-risk intervals for MZT and opens the door to performing a more detailed investigation, to identify the mechanisms responsible for the spikes of MZT incidence.

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Assessment of day-3 morphology and euploidy for individual chromosomes in embryos that develop to the blastocyst stage

Cited by 45 publications

References 40 publications

In vitro fertilization in women under 35: counseling should differ by age

In vitro fertilization in women under 35: counseling should differ by age

Blastocyst morphology holds clues concerning the chromosomal status of the embryo

Clustering of monozygotic twinning in IVF

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