Assessment of cytomegalovirus infections using neopterin and a new immunoblot

Jungraithmayr, Therese; Reschke, M.; Grebe, Scott O.; Lange, H.; Radsak, K.; Mueller, Thomas

doi:10.1016/s0009-8981(01)00528-9

Cited by 22 publications

(18 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Macrophage activation could translate to increased neopterin production. This is supported by the observation that increased serum and urine neopterin correlated with the appearance of symptoms or signs of CMV infection, preceding CMV‐specific immunoglobulin M response by a median of 9 days [35].…”

Section: Discussionmentioning

confidence: 83%

The value of serum neopterin, interferon-gamma levels and interleukin-12B polymorphisms in predicting acute renal allograft rejection

Chin

Adams

Carey

et al. 2008

Clinical and Experimental Immunology

View full text Add to dashboard Cite

SummaryAcute rejection remains a poor predictor of graft outcome. In this study, we measured serum levels of interferon (IFN)-g and neopterin by enzyme-linked immunosorbent assay and a single nucleotide polymorphism (SNP) within the 3Ј untranslated region of the interleukin (IL)-12 B gene (1188 A/C) to determine whether either of these factors could predict acute rejection in renal transplantation. Significantly higher early post-transplant neopterin levels (days 5-7; 35·7 versus 19·9 nmol/l) were observed in recipients who subsequently rejected their grafts. Post-transplant neopterin levels showed a strong positive correlation with 1-month creatinine levels (Spearman's correlation 0·62, P < 0·001), suggesting macrophage activation early after transplantation. Pretransplant neopterin and IFN-g levels and the IL-12B gene SNP did not predict acute rejection in this small retrospective study. The ability to predict acute rejection non-invasively early after transplantation could lead to individual tailoring of immunosuppressive regimens and perhaps lead eventually to longer graft survival.

show abstract

Section: Discussionmentioning

confidence: 83%

The value of serum neopterin, interferon-gamma levels and interleukin-12B polymorphisms in predicting acute renal allograft rejection

Chin

Adams

Carey

et al. 2008

Clinical and Experimental Immunology

View full text Add to dashboard Cite

show abstract

“…However, immunosuppressed populations, such as end-stage renal disease patients or transplant recipients, have an increased burden of low-grade, chronic viral replication (38,(48)(49)(50). This remains hypothetical, as we did not monitor NEOP levels or rates of CMV sero-conversion in the long term.…”

Section: Discussionmentioning

confidence: 99%

Macrophage activation is associated with poorer long‐term outcomes in renal transplant patients

Grebe

Kuhlmann

Fogl

et al. 2010

Clinical Transplantation

View full text Add to dashboard Cite

Long-term graft and patient survival after renal transplantation are largely determined by progression of chronic allograft dysfunction and cardiovascular disease. Inflammation plays a crucial role in both disease processes. We prospectively analyzed the association of early peri-transplant inflammatory burden on long-term outcomes in 144 consecutive deceased donor renal allograft recipients. Single time point and cumulative levels of markers of acute phase response (serum amyloid A [SAA] and C-reactive protein [SCRP]) and macrophage activation (serum and urine neopterin) were measured daily during the immediate post-operative period. Mean patient follow-up was 16 yr. Graft and patient survival rates at one-, five-, and 10-yr were 90%, 70%, and 51%, and 97%, 77%, and 59%, respectively. Graft loss occurred in 90 patients, of whom 71 died with a functioning graft and 19 returned to dialysis. CRP, SAA and neopterin (NEOP) levels were all elevated post-operatively. High levels of NEOP, in contrast to SAA or SCRP, were associated with poorer graft and patient survival (p < 0.05), specifically with death from cardiovascular events and cytomegalovirus IgG positivity. These findings strongly suggest that early post-transplant macrophage activation, as reflected by NEOP levels, is associated with poorer long-term graft and patient survival.

show abstract

“…The extensive accumulation of these cells in CMV-infected elderly persons leads to an increase in the IFN-␥ production of the total CD8 ϩ T-cell population (Fig. 2) and consequently to an increase in the whole-body load of IFN-␥, as indicated by high neopterin serum concentrations during CMV infection (17). A high type 1 combined with a low type 2 cytokine production (Fig.…”

Section: Cd28mentioning

confidence: 96%

Long-Term Cytomegalovirus Infection Leads to Significant Changes in the Composition of the CD8⁺T-Cell Repertoire, Which May Be the Basis for an Imbalance in the Cytokine Production Profile in Elderly Persons

Almanzar

Schwaiger

Jenewein

et al. 2005

J Virol

321

272

View full text Add to dashboard Cite

In spite of the present belief that latent cytomegalovirus (CMV) infection drives CD8؉ T-cell differentiation and induces premature immune senescence, no systematic studies have so far been performed to compare phenotypical and functional changes in the CD8 ؉ T-cell repertoire in CMV-infected and noninfected persons of different age groups. In the present study, number, cytokine production, and growth potential of naïve (CD45RA ؉ CD28 ؉ ), memory (CD45RA ؊ CD28 ؉ ), and effector (CD45RA؉ T cells were analyzed in young, middle-aged, and elderly clinically healthy persons with a positive or negative CMV antibody serology. Numbers and functional properties of CMVpp65 495-503 -specific CD8 ؉ T cells were also studied. We demonstrate that aging as well as CMV infection lead to a decrease in the size of the naïve CD8 ؉ T-cell pool but to an increase in the number of CD8؉ effector T cells, which produce gamma interferon but lack substantial growth potential. The size of the CD8 ؉ memory T-cell population, which grows well and produces interleukin-2 (IL-2) and IL-4, also increases with aging, but this increase is missing in CMV carriers. Life-long latent CMV infection seems thus to diminish the size of the naïve and the early memory T-cell pool and to drive a Th1 polarization within the immune system. This can lead to a reduced diversity of CD8 responses and to chronic inflammatory processes which may be the basis of severe health problems in elderly persons.

show abstract

Assessment of cytomegalovirus infections using neopterin and a new immunoblot

Cited by 22 publications

References 28 publications

The value of serum neopterin, interferon-gamma levels and interleukin-12B polymorphisms in predicting acute renal allograft rejection

The value of serum neopterin, interferon-gamma levels and interleukin-12B polymorphisms in predicting acute renal allograft rejection

Macrophage activation is associated with poorer long‐term outcomes in renal transplant patients

Long-Term Cytomegalovirus Infection Leads to Significant Changes in the Composition of the CD8⁺T-Cell Repertoire, Which May Be the Basis for an Imbalance in the Cytokine Production Profile in Elderly Persons

Contact Info

Product

Resources

About

Assessment of cytomegalovirus infections using neopterin and a new immunoblot

Cited by 22 publications

References 28 publications

The value of serum neopterin, interferon-gamma levels and interleukin-12B polymorphisms in predicting acute renal allograft rejection

The value of serum neopterin, interferon-gamma levels and interleukin-12B polymorphisms in predicting acute renal allograft rejection

Macrophage activation is associated with poorer long‐term outcomes in renal transplant patients

Long-Term Cytomegalovirus Infection Leads to Significant Changes in the Composition of the CD8+T-Cell Repertoire, Which May Be the Basis for an Imbalance in the Cytokine Production Profile in Elderly Persons

Contact Info

Product

Resources

About

Long-Term Cytomegalovirus Infection Leads to Significant Changes in the Composition of the CD8⁺T-Cell Repertoire, Which May Be the Basis for an Imbalance in the Cytokine Production Profile in Elderly Persons