Assessment of Cytomegalovirus DNA and Protein Expression in Patients with Myocarditis

Schönian, U; Crombach, M.; Maisch, Bernhard

doi:10.1006/clin.1993.1123

Cited by 33 publications

(17 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Our protocol has been previously published (8,10,12). In brief, after deparaffinization, sections were incubated with CD45 or CD68 (DAKO, Carpinteria, CA).…”

Section: Immunohistochemistrymentioning

confidence: 99%

“…In cases of sudden, unexpected death, serology is usually not available, and viral cultures have relatively low sensitivity, especially in the autopsy setting (8). In situ hybridization analyses for DNA viruses, which are associated with high copy number, show both high sensitivity and specificity, as documented in cases of cytomegalovirus and adenovirus-associated myocarditis (12)(13)(14). However, most cases of viral myocarditis are associated with RNA viruses, in which the viral genome number may be below the detection threshold of the assay, which is about 10 copies per cell (15)(16)(17).…”

mentioning

confidence: 99%

See 1 more Smart Citation

Histologic and In Situ Viral Findings in the Myocardium in Cases of Sudden, Unexpected Death

Cioc

Nuovo

2002

Mod Pathol

View full text Add to dashboard Cite

show abstract

“…Our protocol has been previously published (8,10,12). In brief, after deparaffinization, sections were incubated with CD45 or CD68 (DAKO, Carpinteria, CA).…”

Section: Immunohistochemistrymentioning

confidence: 99%

mentioning

confidence: 99%

Histologic and In Situ Viral Findings in the Myocardium in Cases of Sudden, Unexpected Death

Cioc

Nuovo

2002

Mod Pathol

View full text Add to dashboard Cite

show abstract

“…Primer pairs specific for the following gene segment were used for amplification and/or reverse transcription [2,4,9,11,23,26,27]:…”

Section: Molecular Biological Analysesmentioning

confidence: 99%

Cytokine Activation in Pericardial Fluids in Different Forms of Pericarditis

Pankuweit

Wädlich²,

Meyer³

et al. 2000

Herz

Self Cite

View full text Add to dashboard Cite

There are many causes of pericardial effusion and it is useful to classify them etiologically, since this disorder is the most common pathologic process involving the pericardium. This report details our experience with pericardioscopy and epicardial biopsy in 101 patients with pericardial effusions in whom pericardioscopy was performed. By means of clinical data and polymerase chain reaction we tried to elucidate the etiology of the pericardial effusion which were classified as follows: we found 41 effusions to be induced by primary malignant tumors or tumors metastatic to the pericardium. Specific diagnosis of viral and bacterial pericarditis was established in 17 patients by examination of the pericardial effusion with PCR, where we found 3 patients positive for adenovirus, 5 patients positive for cytomegalovirus, 2 patients positive for enterovirus-RNA and 5 patients positive for borrelia Burgdorferi-DNA. Additionally, idiopathic effusions (lymphocytic and autoreactive) were seen in 35 patients. In summary immunological and molecular biology investigations seem to provide an additional tool in the diagnostic of pericardial effusion with unknown etiology. If we focus on the ELISA results, there is some evidence, that the demonstration [table: see text] of activation markers and soluble mediators of inflammation such as Il-6, Il-8 and IFN-gamma in pericardial effusion and the simultaneously lack of these mediators in sera of the patients first may be helpful in the discrimination of autoreactive and lymphocytic effusion. Second, this cytokine pattern or distribution indicates a possible local inflammatory process, where these cytokines were all released from activated T lymphocytes present in lymphocytic effusion. In the future, this may have therapeutic implications.

show abstract

“…Dilated Cardiomyopathy The prevalence of cytomegaloviral DNA in endomyocardial biopsies from patients with myocarditis by PCR or nested PCR ranged from 3% (low) in the analyses by Martin et al [24] and Towbin et al [40] to 38% (high) in the analyses by Schönian et al [37]. In patients with dilated cardiomyopathy the prevalence of cytomegaloviral genome ranged from 0% (low) in the analyses by Towbin et al [40] to 40% (high) in the analyses by Schönian et al [37].…”

Section: Cytomegaloviral Persistence In Myocarditis Andmentioning

confidence: 99%

Prevalence of Viral Genome in Endomyocardial Biopsies from Patients with Inflammatory Heart Muscle Disease

Pankuweit

Portig²,

Eckhardt³

et al. 2000

Herz

Self Cite

View full text Add to dashboard Cite

In the report of the 1995 World Health Federation/International Society and Federation of Cardiology (WHF/ISFC) Task Force on the Definition and Classification of Cardiomyopathies, the definition of heart muscle diseases was updated. Idiopathic, autoimmune, and infectious forms of inflammatory cardiomyopathy are now recognized in this definition. Enteroviruses, adenoviruses and cytomegaloviruses are considered as main etiopathological factors in the pathogenesis of inflammatory heart disease. A wide range of different assays have been and are currently being used, either alone or in combination, to assay for the presence of enteroviral RNA and/or DNA of cytomegalo- and adenoviruses in endomyocardial biopsy and explanted heart samples. The prevalence of cardiotropic viruses in endomyocardial biopsies of patients with clinically suspected inflammatory cardiomyopathy varies widely: enteroviral genome was detected in endomyocardial biopsies of 3 to 53% of patients, cytomegaloviral DNA was detected in 3 to 40% of patients with inflammatory heart disease and adenoviruses in 3 to 23% of the patients. This report summarizes the methods that have been used and the results of molecular biological investigation with polymerase chain reaction, which were reported by several groups over the last years. Taking this together it seems to be clear that the improvement of molecular biological techniques and the experience of people working with these methods will lead to more reliable results on prevalence, persistence and the diagnostic value of these investigations. These findings have to be taken into account in future diagnostic and therapeutic studies in the field of cardiomyopathies.

show abstract

Assessment of Cytomegalovirus DNA and Protein Expression in Patients with Myocarditis

Cited by 33 publications

References 0 publications

Histologic and In Situ Viral Findings in the Myocardium in Cases of Sudden, Unexpected Death

Histologic and In Situ Viral Findings in the Myocardium in Cases of Sudden, Unexpected Death

Cytokine Activation in Pericardial Fluids in Different Forms of Pericarditis

Prevalence of Viral Genome in Endomyocardial Biopsies from Patients with Inflammatory Heart Muscle Disease

Contact Info

Product

Resources

About