“…A second direction of research in this domain refers to the development and application of different techniques to analyze occurrence of microleakages, including [ 7 , 8 , 9 , 10 , 11 ]: (i) penetration with different dyes, such as fuchsin or methylene blue; (ii) testing of marginal integrity by using penetration with radioactive isotopes, bacteria, or using the pressure of a compressed air source; (iii) electronic and electrochemical microleakage monitoring; (iv) imaging assessments with scanning electron microscopy (SEM) [ 12 ], replication and SEM, fluorescent, confocal or stereomicroscopy [ 13 , 14 , 15 ], as well as optical coherence tomography (OCT) [ 16 , 17 , 18 , 19 , 20 , 21 , 22 ].…”