Assessment of cryptic species diversity within blooms and cyst bank of the Alexandrium tamarense complex (Dinophyceae) in a Mediterranean lagoon facilitated by semi-multiplex PCR

Genovesi, Benjamin; Shin-Grzebyk, Mi-Sun; Grzebyk, Daniel; Laabir, Mohamed; Gagnaire, Pierre‐Alexandre; Vaquer, André; Pastoureaud, Annie; Lasserre, Bernard; Collos, Yves; Berrebi, Patrick; Masseret, Estelle

doi:10.1093/plankt/fbq127

Cited by 32 publications

(20 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Such difference previously observed [6,7,16,17] could not be explained by morphological characteristics (size, shape) of the fed dinoflagellate species as they are quite similar [18]. We showed that the CR of C. gigas fed with both toxic and non-toxic Alexandrium species decreased between 6 and 29 h (Figure 1).…”

Section: Resultssupporting

confidence: 53%

Paralytic Toxins Accumulation and Tissue Expression of α-Amylase and Lipase Genes in the Pacific Oyster Crassostrea gigas Fed with the Neurotoxic Dinoflagellate Alexandrium catenella

et al. 2012

View full text Add to dashboard Cite

The pacific oyster Crassostrea gigas was experimentally exposed to the neurotoxic Alexandrium catenella and a non-producer of PSTs, Alexandrium tamarense (control algae), at concentrations corresponding to those observed during the blooming period. At fixed time intervals, from 0 to 48 h, we determined the clearance rate, the total filtered cells, the composition of the fecal ribbons, the profile of the PSP toxins and the variation of the expression of two α-amylase and triacylglecerol lipase precursor (TLP) genes through semi-quantitative RT-PCR. The results showed a significant decrease of the clearance rate of C. gigas fed with both Alexandrium species. However, from 29 to 48 h, the clearance rate and cell filtration activity increased only in oysters fed with A. tamarense. The toxin concentrations in the digestive gland rose above the sanitary threshold in less than 48 h of exposure and GTX6, a compound absent in A. catenella cells, accumulated. The α-amylase B gene expression level increased significantly in the time interval from 6 to 48 h in the digestive gland of oysters fed with A. tamarense, whereas the TLP gene transcript was significantly up-regulated in the digestive gland of oysters fed with the neurotoxic A. catenella. All together, these results suggest that the digestion capacity could be affected by PSP toxins.

show abstract

Section: Resultssupporting

confidence: 53%

Paralytic Toxins Accumulation and Tissue Expression of α-Amylase and Lipase Genes in the Pacific Oyster Crassostrea gigas Fed with the Neurotoxic Dinoflagellate Alexandrium catenella

et al. 2012

View full text Add to dashboard Cite

show abstract

“…Similarly, multiplex PCR assays have been developed, based upon primers designed from the D1/D2 and ITS regions, for the simultaneous detection and quantification of Alexandrium species coexisting in French and Japanese waters (Guillou et al, 2002; Genovesi et al, 2011; Nagai, 2011) and Alexandrium cysts in bottom sediments (Erdner et al, 2010).…”

Section: Alexandrium Speciesmentioning

confidence: 99%

The globally distributed genus Alexandrium: Multifaceted roles in marine ecosystems and impacts on human health

et al. 2012

View full text Add to dashboard Cite

The dinoflagellate genus Alexandrium is one of the major harmful algal bloom (HAB) genera with respect to the diversity, magnitude and consequences of blooms. The ability of Alexandrium to colonize multiple habitats and to persist over large regions through time is testimony to the adaptability and resilience of this group of species. Three different families of toxins, as well as an as yet incompletely characterized suite of allelochemicals are produced among Alexandrium species. Nutritional strategies are equally diverse, including the ability to utilize a range of inorganic and organic nutrient sources, and feeding by ingestion of other organisms. Many Alexandrium species have complex life histories that include sexuality and often, but not always, cyst formation, which is characteristic of a meroplanktonic life strategy and offers considerable ecological advantages. Due to the public health and ecosystem impacts of Alexandrium blooms, the genus has been extensively studied, and there exists a broad knowledge base that ranges from taxonomy and phylogeny through genomics and toxin biosynthesis to bloom dynamics and modeling. Here we present a review of the genus Alexandrium, focusing on the major toxic and otherwise harmful species.

show abstract

“…Implications in estimating genetic diversity in a natural Alexandrium bloom SSU rDNA has been commonly employed to analyze species or genetic diversities in natural assemblages of phytoplankton (Lee et al, 2010;Santos et al, 2010;Luo et al, 2011 as recent examples). Recent studies also used this method to determine the genotypic composition of harmful algal blooms Long et al, 2007;Genovesi et al, 2011). It is interesting to find that during the 2008 toxic bloom of Alexandrium in Northport Harbor, New York, there was a relatively diverse dinoflagellate community including non-Alexandrium taxa related to the parasitic genus Amoebophrya, Heterocapsa triquetra, Gymnodinium sp.…”

Section: High-irp and No/low-irp Clades And Potential Species Boundariesmentioning

confidence: 99%

Phylogenetic analysis guided by intragenomic SSU rDNA polymorphism refines classification of “Alexandrium tamarense” species complex

et al. 2012

View full text Add to dashboard Cite

Assessment of cryptic species diversity within blooms and cyst bank of the Alexandrium tamarense complex (Dinophyceae) in a Mediterranean lagoon facilitated by semi-multiplex PCR

Cited by 32 publications

References 38 publications

Paralytic Toxins Accumulation and Tissue Expression of α-Amylase and Lipase Genes in the Pacific Oyster Crassostrea gigas Fed with the Neurotoxic Dinoflagellate Alexandrium catenella

Paralytic Toxins Accumulation and Tissue Expression of α-Amylase and Lipase Genes in the Pacific Oyster Crassostrea gigas Fed with the Neurotoxic Dinoflagellate Alexandrium catenella

The globally distributed genus Alexandrium: Multifaceted roles in marine ecosystems and impacts on human health

Phylogenetic analysis guided by intragenomic SSU rDNA polymorphism refines classification of “Alexandrium tamarense” species complex

Contact Info

Product

Resources

About