Assessment of copper and zinc salts as selectors of antibiotic resistance in Gram-negative bacteria

Becerra-Castro, Cristina; Machado, Rita A.; Vaz‐Moreira, Ivone; Manaia, Célia M.

doi:10.1016/j.scitotenv.2015.05.102

Cited by 52 publications

(32 citation statements)

References 32 publications

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“…Because of the extended use of Cu‐derived compounds as antifungals in olive tree agricultural fields in the province of Jaén, this metal is expected to be the main one responsible for the propagation of antibiotic resistance in this environment, as previously established . The aim of the present study was to isolate and identify metal‐tolerant bacteria from soils in olive tree fields routinely treated with Cu‐derived compounds.…”

Section: Introductionmentioning

confidence: 91%

“…10 There are clearly established links between the resistance genes found in soil and animals and resistance genes found in humans, including human pathogenic bacteria, with antibiotic resistance genes found in the former also present in human pathogens. 11,12 These links have been seen in diverse environments that include industrial, 13,14 clinical, 15 aquatic 13,16 (including wastewater) 17 and, especially, agricultural [18][19][20][21][22] settings.…”

Section: Introductionmentioning

confidence: 99%

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Copper tolerance and antibiotic resistance in soil bacteria from olive tree agricultural fields routinely treated with copper compounds

et al. 2019

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BACKGROUND Heavy metal pollution may act as persistent selective pressure that favors the spread of antimicrobial resistance in natural environments. The aim of this study was to isolate and identify metal‐tolerant bacteria from soils in olive tree fields routinely treated with copper‐derived compounds and to evaluate the tolerance of bacterial strains to other metals and their resistance to clinically relevant antibiotics. RESULTS Five hundred and ninety‐five bacterial isolates from 45 olive tree agricultural fields were studied. Minimum inhibitory concentrations (MICs) ≥ 16 mmol L−1 were detected for copper (57% of isolates), zinc (37%) and lead (62%), while only 3% had MICs ≥ 12 mmol L−1 for nickel. Ninety‐six metal‐tolerant strains were selected for identification and antibiotic resistance determination. Most isolates belonged to the genera Pseudomonas (37%), Bacillus (23%) and Chryseobacterium (20%), while 6% were identified as Variovorax, 4% as Stenotrophomonas and 2% as Serratia or Burkholderia. Highest copper tolerance was detected among Pseudomonas. Over 75% of the strains with high copper tolerance were also resistant to vancomycin, 50% to ampicillin and 40% to erythromycin or trimethoprim/sulfamethoxazole. CONCLUSION Bacteria from olive soils are tolerant to metals, mainly copper, but also zinc and lead, as well as resistant to clinically important antibiotics, which could be a troublesome issue in clinical settings. © 2019 Society of Chemical Industry

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Section: Introductionmentioning

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Copper tolerance and antibiotic resistance in soil bacteria from olive tree agricultural fields routinely treated with copper compounds

et al. 2019

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“…Consistently with this view, sub-inhibitory concentrations of heavy metals have been shown to be sufficient for selecting bacterial populations harboring multi-resistant plasmids with heavy-metal resistances [27] . Concentrations of copper around 1 mM have some effect on Gram-negative sewage bacteria [28] . In fact our study was designed to observe the effect of these relatively low copper concentrations (0 -2.05 mM).…”

Section: Discussionmentioning

confidence: 99%

Phenotypic Copper Resistance in Aerobic Intestinal Bacteria From Children With Different Levels of Copper-Exposure

Baquero¹,

Sanchez-Valenzuelaa#²,

Ripolla³

et al. 2017

JEHS

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Copper is an environmental element, essential for life, to which humans are exposed by inhalation, hand-to-mouth contamination, or ingestion of food and water. In this work, bacterial copper susceptibility (amount of copper able to inhibit bacterial growth) of aerobic bacteria from the intestinal microbiota of healthy children in Spain was explored. To establish the possible effect of children's exposure to copper in the selection of copper-resistant organisms, the prevalence of copper-resistance among bacteria isolated in stool samples of 233 children belonging to the INMA cohort of the Spanish Project for Environment and Childhood Research was studied. Stool samples were seeded into Szybalsky-type agar plates containing specific culture media and a gradient of copper sulphate (< 0.68 -2.05 mM). Culture media was suitable for growth of Gamma-Proteobacteria (mostly Enterobacteriaceae), Enterococcus and Staphylococcus. Colonies growing at intermediate (IR: < 0.68 -1.36 mM) and/or higher copper concentrations (HR: 1.36 -2.05 mM) were characterized by MaldiTOF assays. Sixty different species of copper-resistant organisms were detected. For Enterobacteriaceae, HR colonies were detected in 64.5% of seeded fecal samples, mostly corresponding to genus Escherichia (77% of positive samples); Enterobacter (15%), Citrobacter (13%), and Klebsiella (4.7%). However, Escherichia coli populations have a significantly lower proportion of high copper resistant colonies (49%) than the ensemble of Klebsiella-Enterobacter-Citrobacter colonies (77%). In HR-colonies of Firmicutes, Enterococcus genus was found in 97.3% of fecal samples, predominantly E. faecium (86%, of the positive Enterococcus samples), E. faecalis (37%), and E. hirae (6.9%). No significant correlation was found between counts of HR-colonies and the copper concentrations found in dry hair of the children studied (10 -30 mcg/g). Copper-resistant populations in the children's intestine might have evolved in the copper-rich external environment.

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“…Ralstonia pickettii has been detected in water, plants, soils, and biological fluids (1–6). Infections caused by members of this species suggest their opportunistic character (6).…”

Section: Genome Announcementmentioning

confidence: 99%

“…However, some resistance phenotypes, particularly those against aminoglycosides, vary within the species, suggesting that this resistance can be an acquired characteristic. This was a motivation to analyze the genomes of two isolates of R. pickettii , recovered from hospital effluent and yielding distinct aminoglycoside resistance phenotypes (1). …”

Section: Genome Announcementmentioning

confidence: 99%

Draft Genome Sequences of Two Ralstonia pickettii Strains with Different Aminoglycoside Resistance Phenotypes

et al. 2016

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Assessment of copper and zinc salts as selectors of antibiotic resistance in Gram-negative bacteria

Cited by 52 publications

References 32 publications

Copper tolerance and antibiotic resistance in soil bacteria from olive tree agricultural fields routinely treated with copper compounds

Copper tolerance and antibiotic resistance in soil bacteria from olive tree agricultural fields routinely treated with copper compounds

Phenotypic Copper Resistance in Aerobic Intestinal Bacteria From Children With Different Levels of Copper-Exposure

Draft Genome Sequences of Two Ralstonia pickettii Strains with Different Aminoglycoside Resistance Phenotypes

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