Assessment of conventional PCR and real-time PCR compared to the gold standard method for screening Streptococcus agalactiae in pregnant women

Ferreira, Michele Berger; de‐Paris, Fernanda; Paiva, Rodrigo Minuto; Nunes, Luciana Neves

doi:10.1016/j.bjid.2018.09.005

Cited by 12 publications

(12 citation statements)

References 40 publications

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“…In contrast to culture which is a considered as a gold standard tool, 77.3% more detection capacity was offered by PCR [21]. In agreement to our study, other studies in Brazil (3.8% vs. 29.2%) [22], Iran (27.7% vs. 43.8%) [23], China (0% vs. 50.7%) [24] and Jimma, Ethiopia (3% vs. 33%) were also indicated the sensitivity of PCR over culture [25]. � Normal values: WCC = 0-5/μL, CSF Protein = 40-100 mg/dl and CSF glucose = 40-75 mg/dl [13,14].…”

Section: Discussionsupporting

confidence: 91%

Enhanced identification of Group B streptococcus in infants with suspected meningitis in Ethiopia

et al. 2020

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Meningitis is one of the top ten causes of death among Ethiopian infants. Group B streptococcus (GBS) has emerged as a leading cause of meningitis in neonates and young infants, resulting in high mortality. Despite this, there is no report on GBS associated meningitis in Ethiopia where infant meningitis is common. Hence, the aim of this study was to determine the proportion of GBS associated meningitis among Ethiopian infants. PCR was prospectively used to detect GBS in culture-negative cerebrospinal fluid (CSF) samples, which were collected from infants suspected for meningitis, at Tikur Anbessa specialized hospital, Ethiopia, over a one-year period. GBS was detected by PCR in 63.9% of culture-negative CSF samples. Out of the 46 GBS positive infants, 10.9% (n = 5) of them died. The late onset of GBS (LOGBS) disease was noted to have a poor outcome with 3 LOGBS out of 5 GBS positive samples collected from patients with the final outcome of death. PCR was advantageous in the identification of GBS in culture-negative CSF samples. GBS was detected in 64% of the CSF samples from infants with meningitis compared with zero-detection rate by culture.

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Section: Discussionsupporting

confidence: 91%

Enhanced identification of Group B streptococcus in infants with suspected meningitis in Ethiopia

et al. 2020

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“…Currently, bacterial culture is the gold standard for the identification of GBS infection, but it is timeconsuming with limited sensitivity and vulnerable to interference [18]. Also, real-time qPCR requires expensive equipment and only quantifies nucleic acid relatively.…”

Section: Discussionmentioning

confidence: 99%

Development of a droplet digital PCR method for detection of Streptococcus agalactiae

Zeng

Chen

et al. 2020

BMC Microbiol

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Background: Streptococcus agalactiae (GBS) is the causative pathogen of puerperal sepsis in pregnant women and pneumonia, sepsis and meningitis in infants. Infection of GBS is responsible for the increased morbidity in pregnant women and the elderly, and bring challenges to clinical diagnosis and treatment. However, culture-based approaches to detect S.agalactiae is time-consuming with limited sensitivity. Besides, real-time quantitative PCR demands expensive instruments with tedious steps. Thus, we aim to establish a new detection method for more accurate and rapid detection of S.agalactiae. Results: The ddPCR primer targeted the CpsE gene showed better amplified efficiency in the reaction. The limit of detection for GBS DNA with ddPCR was able to reach 5 pg/μL. Moreover, no positive amplified signals could be detected in the reactions which served 11 non-GBS strains DNA as templates. Furthermore, the coefficient of variation of this method was 4.5%, indicating excellent repeatability of ddPCR assay. Conclusions: In our study, ddPCR was performed as a rapid detection of S.agalactiae with high sensitivity and specificity. This technique can promote the accuracy of the diagnosis of GBS infection and provide a scientific basis for clinical treatment.

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“…Nowadays, a bacterial culture is still a gold standard method to identify GBS infection, but it is timeconsuming with limited sensitivity and vulnerable to interference [17] . Also, real-time qPCR requires expensive equipment and only quantifies nucleic acid relatively.…”

Section: Discussionmentioning

confidence: 99%

Development of a reliable method to diagnose Streptococcus agalactiae infection by droplet digital PCR

Guo¹,

Zeng

Chen

et al. 2020

Preprint

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Background: Streptococcus agalactiae (GBS) is the causative pathogen of puerperal sepsis in pregnant women and meningitis in infants. Infection of GBS is responsible for the increased morbidity in pregnant women and the elderly, and bring challenges to clinical diagnosis and treatment. However, culture-based approaches to detect S.agalactiae is time-consuming with limited sensitivity. Besides, real-time quantitative PCR demand for expensive instruments with tedious steps. Thus, we aim to establish a new detection method for more accurate and rapid detection of S.agalactiae.Results: The ddPCR primer targeted the CspE gene showed a better amplified efficiency in the reaction. The limitation of ddPCR for identifying GBS DNA was able to reach 5 pg/µL. Moreover, no positive amplified signals could be detected in the reactions which served 11 non-GBS strains DNA as templates. Furthermore, the coefficient of variation of this method is 4.5%, indicating an excellent repeatability of ddPCR assay.Conclusions: In our study, ddPCR was performed as a rapid detection of S.agalactiae with high sensitivity and specificity. This technique can promote the accuracy of the diagnosis of GBS infection and provide a scientific basis for clinical treatment.

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Assessment of conventional PCR and real-time PCR compared to the gold standard method for screening Streptococcus agalactiae in pregnant women

Cited by 12 publications

References 40 publications

Enhanced identification of Group B streptococcus in infants with suspected meningitis in Ethiopia

Enhanced identification of Group B streptococcus in infants with suspected meningitis in Ethiopia

Development of a droplet digital PCR method for detection of Streptococcus agalactiae

Development of a reliable method to diagnose Streptococcus agalactiae infection by droplet digital PCR

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