Assessment of constituents in Allium by multivariate data analysis, high-resolution α-glucosidase inhibition assay and HPLC-SPE-NMR

Schmidt, Jeppe S.; Nyberg, Nils T.; Stærk, Dan

doi:10.1016/j.foodchem.2014.03.062

Cited by 52 publications

(22 citation statements)

References 32 publications

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“…It can reduce the risk of the serious neurological and cardiovascular complications through the inhibition of carbohydrate hydrolyzing enzymes, such as a-glucosidase in the epithelium of small intestine (Schmidt, Nyberg, & Staerk, 2014). This enzyme plays a vital role in maintaining the normal physiological function and participates in carbohydrate metabolism that specifically hydrolyzes the a-glucopyranoside bond to release a-glucose from the non-reducing end of the sugar (Lordan, Smyth, Soler-Vila, Stanton, & Ross, 2013).…”

Section: Introductionmentioning

confidence: 99%

Inhibitory kinetics and mechanism of kaempferol on α-glucosidase

et al. 2016

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Section: Introductionmentioning

confidence: 99%

Inhibitory kinetics and mechanism of kaempferol on α-glucosidase

et al. 2016

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“…As HPLC-HRMS-SPE-NMR only allows for chemical analysis, the recent combination with semi-high-resolution and/or high-resolution bioactivity profiling, i.e., microfractionation in microplates followed by bioassaying to yield a semi-high or high-resolution inhibition profile, is one of the most promising technological developments within bioanalytical plant research. The resulting HR-bioassay/HPLC-HRMS-SPE-NMR technology platform has already proven effective for fast identification of α-glucosidase inhibitors (Schmidt et al, 2012, 2014; Kongstad et al, 2015; Wubshet et al, 2015), aldose reductase inhibitors (Tahtah et al, 2015), α-amylase inhibitors (Okutan et al, 2014), and radical scavengers (Wiese et al, 2013; Wubshet et al, 2013; Liu et al, 2015) directly from crude plant extracts. However, the current work is the first example of semi-high-resolution antileishmanial inhibition profiling coupled with HPLC-HRMS-SPE-NMR.…”

Section: Introductionmentioning

confidence: 99%

Characterization of Antileishmanial Compounds from Lawsonia inermis L. Leaves Using Semi-High Resolution Antileishmanial Profiling Combined with HPLC-HRMS-SPE-NMR

Iqbal

Kongstad

2017

Front. Pharmacol.

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This work describes an analytical platform based on semi-high-resolution antileishmanial profiling combined with hyphenation of high-performance liquid chromatographyhigh-resolution mass spectrometry -solid-phase extraction -nuclear magnetic resonance spectroscopy, i.e., semiHR-antileishmanial assay/HPLC-HRMS-SPE-NMR. The platform enables fast pinpointing of HPLC peaks representing Leishmania tropica inhibitors in complex matrices, with subsequent structural identification of targeted inhibitors. Active analytes were cumulatively trapped on SPE cartridges and the structures elucidated by analysis of NMR spectra obtained in the HPLC-HRMS-SPE-NMR mode. This led to the identification of six known compounds 2,4,6-trihydroxyacetophenone-2-O-β-D-glucopyranoside (1), lalioside (2), luteolin-4 -O-β-Dglucopyranoside (3), apigenin-4 -O-β-D-glucopyranoside (4), luteolin (5), and apigenin (6). IC 50 of the active compounds were determined with luteolin being the most potent inhibitor with an IC 50 value of 4.15 µg/ml. The platform proved to be an efficient method for the identification of L. tropica inhibitors.

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“…However, several side effects have been reported [6,7], which makes the search for new α-glucosidase inhibitors of utmost importance. From this perspective, the very recent development of state-of-the-art high-resolution bioassays combined with hyphenation of high-performance liquid chromatography, high-resolution mass spectrometry, solid-phase extraction and nuclear magnetic resonance spectroscopy [8], i.e., HR-bioassay/HPLC-HRMS-SPE-NMR has been an important tool for identification of α-glucosidase inhibitors from plant and food extracts [9][10][11][12][13][14]-including the development of dual and triple high-resolution inhibition profiling [15][16][17]. Furthermore, the HR-bioassay/HPLC-HRMS-SPE-NMR platform has successfully been applied for identification of α-amylase inhibitors [18], fungal plasma membrane H + -ATPase inhibitors [19], antioxidants [20] and monoamine oxidase A inhibitors [21].…”

Section: Introductionmentioning

confidence: 99%

High-Resolution α-Glucosidase Inhibition Profiling Combined with HPLC-HRMS-SPE-NMR for Identification of Antidiabetic Compounds in Eremanthus crotonoides (Asteraceae)

et al. 2016

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α-Glucosidase inhibitors decrease the cleavage-and absorption rate of monosaccharides from complex dietary carbohydrates, and represent therefore an important class of drugs for management of type 2 diabetes. In this study, a defatted ethyl acetate extract of Eremanthus crotonoides leaves with an inhibitory concentration (IC 50 ) of 34.5 µg/mL towards α-glucosidase was investigated by high-resolution α-glucosidase inhibition profiling combined with HPLC-HRMS-SPE-NMR. This led to identification of six α-glucosidase inhibitors, namely quercetin (16), trans-tiliroside (17), luteolin (19), quercetin-3-methyl ether (20), 3,5-di-O-caffeoylquinic acid n-butyl ester (26) and 4,5-di-O-caffeoylquinic acid n-butyl ester (29). In addition, nineteen other metabolites were identified. The most active compounds were the two regioisomeric di-O-caffeoylquinic acid derivatives 26 and 29, with IC 50 values of 5.93 and 5.20 µM, respectively. This is the first report of the α-glucosidase inhibitory activity of compounds 20, 26, and 29, and the findings support the important role of Eremanthus species as novel sources of new drugs and/or herbal remedies for treatment of type 2 diabetes.

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Assessment of constituents in Allium by multivariate data analysis, high-resolution α-glucosidase inhibition assay and HPLC-SPE-NMR

Cited by 52 publications

References 32 publications

Inhibitory kinetics and mechanism of kaempferol on α-glucosidase

Inhibitory kinetics and mechanism of kaempferol on α-glucosidase

Characterization of Antileishmanial Compounds from Lawsonia inermis L. Leaves Using Semi-High Resolution Antileishmanial Profiling Combined with HPLC-HRMS-SPE-NMR

High-Resolution α-Glucosidase Inhibition Profiling Combined with HPLC-HRMS-SPE-NMR for Identification of Antidiabetic Compounds in Eremanthus crotonoides (Asteraceae)

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