Assessment of collagen content in fish skin – development of a flow analysis method for hydroxyproline determination

Melo, Maria M. P.; Mesquita, Raquel B. R.; Coscueta, Ezequiel R.; Pintado, Manuela E.; Rangel, António O. S. S.

doi:10.1039/d3ay01589k

Cited by 2 publications

(1 citation statement)

References 20 publications

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“…Guan et al (2023) manufactured cultured meat samples by seeding porcine muscle stem cells on soy protein scaffolds and found that the amino acid content and quality of the cultured meat samples increased with the efficiency of myogenic differentiation and myotube formation. In this study, we focused on the nutritional profile of fibroblasts and found that different from previous studies, the addition of fibroblasts significantly elevated the content of collagenspecific amino acid hydroxyproline, and collagen-related amino acids proline and glycine (Shoulders and Raines, 2009;Yuswan et al, 2021;Melo et al, 2023), suggesting the importance of fibroblasts in the manufacture of ECM-rich tissues such as connective tissue and skin. This study is focused on developing cultured meat that mimics the skin part of pork belly, using pig fibroblasts.…”

Section: Discussionmentioning

confidence: 61%

Highly efficient isolation and 3D printing of fibroblasts for cultured meat production

Yang,

Fei,

Wang

et al. 2024

Front. Sustain. Food Syst.

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Fibroblasts are important components of animal tissues such as muscle and skin, as they are the major producers of various matrix proteins. Matrix proteins such as collagen play an important role in meat products by providing unique nutrition, texture, and flavor. Cultured meat is an innovative meat alternative produced by culturing animal cells, but currently, relatively few studies have been conducted using fibroblasts as seed cells for cultured meat manufacturing. In this work, we first developed an innovative digestion-friction method for isolating fibroblasts from porcine skin efficiently and cost-effectively. After optimizing the enzymatic digestion and physical friction conditions, 2.39 ± 0.28 × 105 fibroblasts were obtained from 1 cm2 of porcine skin tissue, which was about 9 times higher than the conventional tissue explant method. In addition, we identified an edible bio-ink composed of gelatin and chitosan that has good printing properties and supports fibroblast adhesion and growth. Furthermore, we fabricated fibroblast-based cultured meat by 3D printing with an initial cell density of 1.0 × 107 mL−1 and evaluated its texture and nutritional properties. This work provides valuable insights and references for introducing fibroblasts into the production of cultured meat that is more comparable to structured animal meat.

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Section: Discussionmentioning

confidence: 61%

Highly efficient isolation and 3D printing of fibroblasts for cultured meat production

Yang,

Fei,

Wang

et al. 2024

Front. Sustain. Food Syst.

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Label-free electrochemical immunosensor based on tailored polyclonal antibody and cobalt hydroxide oxide nanoflakes for detecting ancient leather artifacts

Xu,

Li,

Zhang

et al. 2024

Microchemical Journal

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Assessment of collagen content in fish skin – development of a flow analysis method for hydroxyproline determination

Abstract: Flow injection method for the quantification of hydroxyproline to corelate with collagen content in blue shark skin.

Cited by 2 publications

References 20 publications

Highly efficient isolation and 3D printing of fibroblasts for cultured meat production

Highly efficient isolation and 3D printing of fibroblasts for cultured meat production

Label-free electrochemical immunosensor based on tailored polyclonal antibody and cobalt hydroxide oxide nanoflakes for detecting ancient leather artifacts

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