Assessment of circular wound healing in rats after exposure to 808‐nm laser pulses during specific healing phases

Tabakoglu, Ozgur; Sani, Musbahu Muhammad; Uba, Abdullahi Ibrahim; Abdullahi, U.

doi:10.1002/lsm.22462

Cited by 18 publications

(9 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Consistent with this, histological observations also demonstrated enhanced wound repair as reflected by the reduced infiltration of inflammatory cells, increased neo-vascularization, fibrogenesis, collagen accumulation and faster re-epithelialization in 810 nm laser with PW 10 Hz irradiated group. These findings were in agreement with previous studies which have shown that NIR laser (808–830 nm) irradiation significantly increases granulation tissue formation, fibroblasts and collagen deposition on dermal wounds, which lead to accelerated healing processes [19,20,36]. …”

Section: Discussionsupporting

confidence: 93%

Photobiomodulation with Pulsed and Continuous Wave Near-Infrared Laser (810 nm, Al-Ga-As) Augments Dermal Wound Healing in Immunosuppressed Rats

et al. 2016

View full text Add to dashboard Cite

Chronic non-healing cutaneous wounds are often vulnerable in one or more repair phases that prevent normal healing and pose challenges to the use of conventional wound care modalities. In immunosuppressed subject, the sequential stages of healing get hampered, which may be the consequences of dysregulated or stagnant wound inflammation. Photobiomodulation (PBM) or low-level laser (light) therapy (LLLT) emerges as a promising drug-free, non-invasive biophysical approach for promoting wound healing, reduction of inflammation, pain and restoration of functions. The present study was therefore undertaken to evaluate the photobiomodulatory effects of 810 nm diode laser (40 mW/cm2; 22.6 J/cm2) with pulsed (10 and 100 Hz, 50% duty cycle) and continuous wave on full-thickness excision-type dermal wound healing in hydrocortisone-induced immunosuppressed rats. Results clearly delineated that 810 nm PBM at 10 Hz was more effective over continuous and 100 Hz frequency in accelerating wound healing by attenuating the pro-inflammatory markers (NF-kB, TNF-α), augmenting wound contraction (α-SM actin), enhancing cellular proliferation, ECM deposition, neovascularization (HIF-1α, VEGF), re-epithelialization along with up-regulated protein expression of FGFR-1, Fibronectin, HSP-90 and TGF-β2 as compared to the non-irradiated controls. Additionally, 810 nm laser irradiation significantly increased CCO activity and cellular ATP contents. Overall, the findings from this study might broaden the current biological mechanism that could be responsible for photobiomodulatory effect mediated through pulsed NIR 810 nm laser (10 Hz) for promoting dermal wound healing in immunosuppressed subjects.

show abstract

Section: Discussionsupporting

confidence: 93%

Photobiomodulation with Pulsed and Continuous Wave Near-Infrared Laser (810 nm, Al-Ga-As) Augments Dermal Wound Healing in Immunosuppressed Rats

et al. 2016

View full text Add to dashboard Cite

show abstract

“…This confliction might possibly be attributed to different laser parameters, the difference between the employed methods (such as the site of the wound, animal or human study, scales to measure, healing stage) and laser parameters. For example, in the study of Tabakoglu et al, 28 the wound size was half the size of the ulcer examined in the present study; therefore, it is predicted that wound closure in that study was more due to the contraction mechanism rather than healing by secondary intention; and as the wounds in laser group have higher amounts of collagen, the susceptibility to contract is higher in this group than control group. 32 Collagen fibers in laser group showed considerably higher levels than GF group.…”

Section: Discussionmentioning

confidence: 44%

“…There are a large number of studies which demonstrated healing promotion effects for laser. [28][29][30] The mechanism of low-level laser therapy in healing promotion is not completely understood yet; however, it is supposed that laser efficacy is not only due to its heating effects but rather to its photochemical, photophysical and/ or photobiological effects; many name these as photobiomodulation. It seems that laser illumination can improve local circulation, cell proliferation, and collagen synthesis by increasing cell access to ATP.…”

Section: Discussionmentioning

confidence: 99%

Comparative Evaluation of the Efficacy of Laser Therapy and Fibroblastic Growth Factor Injection on Mucosal Wound Healing in Rat Experimental Model

et al. 2018

View full text Add to dashboard Cite

Introduction: The aim of the present study was to compare the effects of laser and basic fibroblastic growth factor (bFGF) treatment on operative wound healing in a rat model. Methods: Sixty-six male Wistar rats were employed in this study. A 10-mm surgical wound was created on the buccal mucosa of each rat, under anesthesia, and then the rats were divided into 3 groups of 22: (1) GF group (received subcutaneous injection of bFGF), (2) laser group (treated with low-level laser irradiation), and (3) control group (received no treatment). On day 5, half of the rats in each group and on day 10 the other half, were sacrificed. Afterward, samples were taken from rats' buccal mucosa for histological assay and scoring. The data were analyzed using Mann-Whitney test (α = 5%). Results: On day 5 there was not any significant difference between GF and control groups; however, the laser group showed clinically delayed wound coverage, compared to other groups (P < 0.05). On day 10, histological examination demonstrated marked vascular granulation tissue (GT) in GF group. Collagen production was significantly prominent in laser group compared to GF treated samples (P = 0.004). Inflammation of GT in GF and laser groups was significantly less than that in control samples (P = 0.005 and P = 0.001, respectively). Conclusion: The components of wound matrix induced by GF and laser treatment were significantly different. Although bFGF or laser treatment of oral wounds, under the conditions of the present study, did not accelerate wound healing, they showed some other notable effects on the quality of healing.

show abstract

“…This study demonstrated in vivo clinical effects of low-level laser therapy (LLLT) on second-degree thermal burns in rats. LLLT is an important intervention for wound healing that has been shown to create beneficial effects on the stimulation of the extracellular matrix, myofibroblasts, and granulation tissue activities for the skin healing process [23][24][25]. The presence of myofibroblasts creates a centripetal contraction from the edges of the burn lesion.…”

Section: Discussionmentioning

confidence: 99%

Anti-inflammatory effects of low-level laser in burn wound models in rats

Kim

2017

PTRS

View full text Add to dashboard Cite

Objective: The anti-inflammatory effects of low-level laser in burn wound model in rats were investigated. Design: Randomized controlled trial. Methods: The rats were assigned to three experimental groups. Group I received second-degree burn wounds; Group II received dressing film and low-level laser (1.2 J/cm 2 ) treatment after a burn wound; Group III received dressing film and low-level laser (2.3 J/cm 2 ) treatment after a burn wound. After inducing a deep second-degree burn wound, the wound was observed every day and the burn area diameter and retraction quantification at 1, 7, and 14 days were evaluated. Low-level laser was investigated on hematological parameters after 14 days. Effects of low-level laser on the inflammatory cytokines (tumor necrosis factor-α [TNF-α] and interleukin-6 [IL-6]) concentrations in the serum were evaluated using immunosorbent assay kits. Results: Group III showed a significant difference in wound size on days 7 and 14 compared to Group I (p<0.05). Group II showed a significant difference in wound size on day 14 compared to Group I (p<0.05). For wound contraction percentage, both laser therapy treatment groups showed a significant difference compared with Group I (p<0.05). There was also a significant difference in wound contraction percentage in Group III compared to Group II (p<0.05). Compared with the model control group, decreased TNF-α and IL-6 levels in the serum was observed at 14 days after burn wound induction. Conclusions:The results of this study suggest that low-level laser therapy can assist in burn wound healing, which might be associated with decreased concentrations of TNF-α and IL-6 related proinflammatory cytokines.

show abstract

Assessment of circular wound healing in rats after exposure to 808‐nm laser pulses during specific healing phases

Cited by 18 publications

References 21 publications

Photobiomodulation with Pulsed and Continuous Wave Near-Infrared Laser (810 nm, Al-Ga-As) Augments Dermal Wound Healing in Immunosuppressed Rats

Photobiomodulation with Pulsed and Continuous Wave Near-Infrared Laser (810 nm, Al-Ga-As) Augments Dermal Wound Healing in Immunosuppressed Rats

Comparative Evaluation of the Efficacy of Laser Therapy and Fibroblastic Growth Factor Injection on Mucosal Wound Healing in Rat Experimental Model

Anti-inflammatory effects of low-level laser in burn wound models in rats

Contact Info

Product

Resources

About