The changes in the structure of lignin
during the pretreatment
processes of biomass can affect its subsequent enzymatic hydrolysis
efficiency. To explore the influence mechanism of dilute acid and
hydrothermal pretreatment of corn stover lignin on cellulose saccharification,
an integrated nondestructive spectral technology with fluorescence
spectroscopy, surface plasmon resonance, and atomic force microscopy
was performed. It showed that the surface lignins on the corn stover
with dilute acid pretreatment at 190 °C (DA190-SL) and hydrothermal
pretreatment at 190 °C (HP190-SL) possessed enhancement (from
76.95 to 80.09%) and inhibition (from 76.95 to 61.91%) for the enzymatic
digestibility of Avicel, respectively. Nondestructive spectroscopic
analysis indicated that HP190-SL adsorbed onto cellulase is mainly
driven by hydrogen bonding and van der Waals forces, while the DA190-SL-enzyme
system was mainly driven by hydrophobic interactions. The association
affinity of DA190-SL combined with cellulase was higher than that
of HP190-SL. The binding force of the HP190-SL-enzyme (0.16 nN) is
lower than that of the DA190-SL-enzyme (0.75 nN), which leads to a
higher propensity for dissociation of HP190-SL from cellulase after
binding. This study aims to establish a theoretical basis for regulating
the enzymatic performance during the hydrothermal and dilute acid
pretreatment of corn stover at the molecular level.