Assessment of CD27 expression as a tool for active and latent tuberculosis diagnosis

Stratification of Latent Mycobacterium tuberculosis Infection by Cellular Immune Profiling

“…However, we did demonstrate that both the TAM-TB assay and the proportions of CD45RA − CD27 − or CD45RA − CD27 + IFN-γ + CD4 + T cells differed between patients with tuberculosis and those with LTBI. We thus independently validated the ability of these signatures for discriminating tuberculosis from LTBI [9, 10], supporting their reproducibility.…”

“…Immune responses to M. tuberculosis in individuals with recently and remotely acquired LTBI were compared using the following 3 M. tuberculosis– specific cellular immune signatures, which have previously been shown to differ between tuberculosis and LTBI: the TNF-α–only T EFF signature [8], the ratio of the median fluorescence intensity of CD27 expression on CD4 + T cells to that of CD27 expression on IFN-γ + CD4 + T cells (determined by the TAM-TB assay [10]), and the proportion of IFN-γ + CD4 + T cells with a CD45RA − CD27 − or CD45RA − CD27 + phenotype [9]. The gating strategies used for each signature are shown in Supplementary Figure 1.…”

“…In this study, 2 signatures incorporating such measurements did not differ significantly between recently acquired and remotely acquired LTBI in our cohort. This indicates that loss of CD27 expression on M. tuberculosis– specific CD4 + T cells, while associated with clinical disease [9–11], is not related to time since infection in patients with LTBI. However, we did demonstrate that both the TAM-TB assay and the proportions of CD45RA − CD27 − or CD45RA − CD27 + IFN-γ + CD4 + T cells differed between patients with tuberculosis and those with LTBI.…”

“…Specifically, we demonstrated that the proportion of purified protein derivative–specific CD4 + T cells secreting tumor necrosis factor α (TNF-α) but not interferon γ (IFN-γ) or interleukin 2 (IL-2) which had a differentiated effector memory (CD45RA − CCR7 − CD127 − ) phenotype (TNF-α–only T EFF ) was able to distinguish tuberculosis from LTBI [8]. Others have also demonstrated that measuring the levels of CD27 expression on M. tuberculosis– specific cytokine-producing CD4 + T cells has the potential to distinguish between tuberculosis and LTBI [9–11]. For example, the TAM-TB assay, which evaluates the ratio of the median fluorescence intensity of CD27 within the whole CD4 + T-cell population to that of CD27 in the M. tuberculosis– specific IFN-γ + CD4 + T cells has been shown to distinguish between tuberculosis in LTBI in children and adults [10].…”

A T‐cell diagnostic test for cystic echinococcosis based on Antigen B peptides

“…Alternative laboratory tests for the diagnosis of chronic infections as tuberculosis or toxoplasmosis are based on cytokines detection upon stimulation of whole blood with pathogen‐derived molecules, including antigenic peptides . We recently set‐up a whole‐blood assay based on interleukin (IL)‐4 detection in response to native‐AgB which showed a good accuracy for CE diagnosis and staging …”