Assessment of cartilage viability in long-term cryopreserved tracheal allografts

Kushibe, Keiji; Takahama, Makoto; Nezu, Kunimoto; Taniguchi, Shigeki

doi:10.1016/s0041-1345(00)02173-4

Cited by 6 publications

(4 citation statements)

References 13 publications

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“…O'Connor 17 reported that homologous cartilage graft stored in the freezer in merthiolate solution did not resorb, even after 6 years. Moreover, Kushibe et al 24 reported that cryopreserved tracheal allografts remained viable for at least 12 months.…”

Section: Discussionmentioning

confidence: 99%

Viability of Cartilage Grafts in Various Forms

Fırat

Gürlek²,

Aydn³

2011

Journal of Craniofacial Surgery

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The viability of cartilage grafts, in many forms, has been researched since the using of cartilage grafts in surgical procedures. Cryopreservation period and viability of cartilage grafts have remained unclear. This study was performed to investigate the durability, viability, and behavior of fresh or cryopreserved cartilage grafts when used as autografts or allografts in various forms.Six cartilage grafts (1 of each preparation type; 3 blocks and 3 diced) were prepared by wrapping with Surgicel or autogenous fascia, or they were left bare. After the graft preparation stage, the cartilage grafts were inserted into pockets prepared on the dorsum of each rabbit. Groups 1, 2, 3, and 4 (6 rabbits in each group) received autogenous fresh grafts, allogenous fresh grafts, autogenous cryopreserved grafts, and allogenous cryopreserved grafts, respectively. All cartilage grafts were implanted for 2 months.At the end of the second month, specimens were harvested and analyzed. The bare grafts provided the most viable specimens. There was no significant difference between the frozen or fresh and allograft or autograft groups with respect to viability and resorption ratios. The bare block graft, in all groups, survived significantly more than the other graft types.Allografts (homografts), similar autografts, did not create major problems, and they had excellent host tolerance and low antigenicity, especially when the perichondrium was removed. Viability and durability of the bare grafts (diced and block) were better than fascia or Surgicel-wrapped cartilage graft forms.

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Section: Discussionmentioning

confidence: 99%

Viability of Cartilage Grafts in Various Forms

Fırat

Gürlek²,

Aydn³

2011

Journal of Craniofacial Surgery

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“…Cryopreserved tracheal allografts have been investigated for cell viability 5 and warm ischemia time 6 in animal models, and successful allotransplantation is reported in mature primates. 7 However, no experimental study regarding the growth of cryopreserved allografts has been reported.…”

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confidence: 99%

Transplantation of the cryopreserved tracheal allograft in growing rabbits

Tanaka

Murakami

Okita

2003

Journal of Pediatric Surgery

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“…However, long storage times exert a deleterious effect on the cartilage. 5 Articular cartilage injuries do not heal, and large defects result in osteoarthritis. Osteochondral transplantation is an effective treatment for long, large defects, but its use is limited by the inability to store cartilage for long periods of time.…”

Section: Introductionmentioning

confidence: 99%

Canine Tracheal Cartilage Cryopreservation: Freezing Injury Is Not Related to Caspase-3 Expression

Sotres-Vega

Santibáñez-Salgado²,

Villalba-Caloca³

et al. 2013

Biopreservation and Biobanking

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Currently, there are no surgical strategies to treat tracheal lesions longer than 7 cm. Such patients are not candidates for tracheal resection or end-to-end anastomosis and are thus left with only repeated palliative procedures to relieve their respiratory insufficiency. Experimental studies using cryopreserved trachea have produced contradictory results, limiting the clinical application of this technique. We evaluated caspase-3 expression and the histological integrity of canine tracheal cartilage cryopreserved using two different solutions, two temperatures, and varying lengths of storage time. Thirty canine tracheal segments of 5 rings were studied. Group 1: Control without cryopreservation. Groups 2 and 4: Cryopreserved in F12K media with 20% fetal bovine serum (FBS) at -70°C for 48 hours. Groups 3 and 5: Cryopreserved in 90% FBS at -70°C for 48 hours. Groups 4 and 5 were then stored for 15 days in liquid nitrogen. All of the segments were thawed, fixed in wax, and cut into rings. Three rings were selected for caspase-3 expression and histological evaluation. Staining of cartilage matrices was significantly modified in the tracheal segments of Group 5. The central region of the cartilage ring was more vulnerable to the effects of freezing than the edges. Under the same cryopreservation temperature and storage time, tracheal cartilage integrity is better preserved when F12K media is used. Caspase-3 expression is not related to cartilage injury from the cryopreservation process.

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Assessment of cartilage viability in long-term cryopreserved tracheal allografts

Cited by 6 publications

References 13 publications

Viability of Cartilage Grafts in Various Forms

Viability of Cartilage Grafts in Various Forms

Transplantation of the cryopreserved tracheal allograft in growing rabbits

Canine Tracheal Cartilage Cryopreservation: Freezing Injury Is Not Related to Caspase-3 Expression

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