Assessment of Cardiovascular Fibrosis Using Novel Fluorescent Probes

Chen, Jiqiu; Lee, Seung Koo; Abd-Elgaliel, Wael R.; Liang, Lifan; Galende, Elisa-Yaniz; Hajjar, Roger J.; Tung, Ching–Hsuan

doi:10.1371/journal.pone.0019097

Cited by 29 publications

(31 citation statements)

References 19 publications

(26 reference statements)

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“…Thus Cna can only bind to collagen triple helix monomers but not to collagen fibers or fibrils. This property has recently been taken advantage of when investigators have used a fluorescently tagged form of Cna to detect monomeric collagen product during fibrosis (45). This specificity also limits the type of collagenous tissue that can be targeted in Cna-dependent adhesion because collagen is primarily found in higher orders of fibrous super structures in healthy mature tissues.…”

Section: Discussionmentioning

confidence: 99%

Collagen-binding Microbial Surface Components Recognizing Adhesive Matrix Molecule (MSCRAMM) of Gram-positive Bacteria Inhibit Complement Activation via the Classical Pathway

Kang

Liang

et al. 2013

Journal of Biological Chemistry

122

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Section: Discussionmentioning

confidence: 99%

Collagen-binding Microbial Surface Components Recognizing Adhesive Matrix Molecule (MSCRAMM) of Gram-positive Bacteria Inhibit Complement Activation via the Classical Pathway

Kang

Liang

et al. 2013

Journal of Biological Chemistry

122

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“…34 Previous studies have used these methods to visualize collagen deposition and measure the size of the scar in diseased cardiac tissues. 3,5,35 We have shown that the scar size measured as percentage of tissue section showing collagen deposition from FT-IRIS analysis strongly correlated with the measurements achieved by trichrome staining of a section from same heart tissues. The fact that samples used in this analysis were comprised of two groups of animals, a treatment group with targeted delivery of VEGF to infarcted myocardium and a control untreated group, demonstrates the applicability of the methodology for different treatment conditions.…”

Section: Discussionmentioning

confidence: 52%

Fourier transform infrared spectroscopic imaging of cardiac tissue to detect collagen deposition after myocardial infarction

et al. 2012

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Abstract. Myocardial infarction often leads to an increase in deposition of fibrillar collagen. Detection and characterization of this cardiac fibrosis is of great interest to investigators and clinicians. Motivated by the significant limitations of conventional staining techniques to visualize collagen deposition in cardiac tissue sections, we have developed a Fourier transform infrared imaging spectroscopy (FT-IRIS) methodology for collagen assessment. The infrared absorbance band centered at 1338 cm −1 , which arises from collagen amino acid side chain vibrations, was used to map collagen deposition across heart tissue sections of a rat model of myocardial infarction, and was compared to conventional staining techniques. Comparison of the size of the collagen scar in heart tissue sections as measured with this methodology and that of trichrome staining showed a strong correlation (R ¼ 0.93). A Pearson correlation model between local intensity values in FT-IRIS and immuno-histochemical staining of collagen type I also showed a strong correlation (R ¼ 0.86). We demonstrate that FT-IRIS methodology can be utilized to visualize cardiac collagen deposition. In addition, given that vibrational spectroscopic data on proteins reflect molecular features, it also has the potential to provide additional information about the molecular structure of cardiac extracellular matrix proteins and their alterations.

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“…The samples were embedded in optimum cutting temperature and stored at Ϫ80°C. Masson's Trichrome was performed as previous described (10). Paraffined samples were used for immunohistochemistry of CD31 (ABCAM, ab28364, dilution 1:100).…”

Section: Methodsmentioning

confidence: 99%

The impact of pressure overload on coronary vascular changes following myocardial infarction in rats

Chen

Petrov

Yaniz‐Galende

et al. 2013

American Journal of Physiology-Heart and Circulatory Physiology

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This study investigates the impact of pressure overload on vascular changes after myocardial infarction (MI) in rats. To evaluate the effect of pressure overload, MI was induced in three groups: 1) left coronary artery ligation for 1 mo (MI-1m), 2) ischemia 30 min/reperfusion for 1 mo (I/R-1m), and 3) ischemia-reperfusion (I/R) was performed after pressure overload induced by aortic banding for 2 mo; 1 mo post-I/R, aortic constriction was released (Ab+I/R+DeAb). Heart function was assessed by echocardiography and in vivo hemodynamics. Resin casting and three-dimensional imaging with microcomputed tomography were used to characterize changes in coronary vasculature. TTC (triphenyltetrazohum chloride) staining and Masson's Trichrome were conducted in parallel experiments. In normal rats, MI induced by I/R and permanent occlusion was transmural or subendocardial. Occluded arterial branches vanished in MI-1m rats. A short residual tail was retained, distal to the occluded site in the ischemic area in I/R-1m hearts. Vascular pathological changes in transmural MI mostly occurred in ischemic areas and remote vasculature remained normal. In pressure overloaded rats, I/R injury induced a sub-MI in which ischemia was transmural, but myocardium in the involved area had survived. The ischemic arterial branches were preserved even though the capillaries were significantly diminished and the pathological changes were extended to remote areas, characterized by fibrosis, atrial thrombus, and pulmonary edema in the Ab+I/R+DeAb group. Pressure overload could increase vascular tolerance to I/R injury, but also trigger severe global ventricular fibrosis and results in atrial thrombus and pulmonary edema.

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Assessment of Cardiovascular Fibrosis Using Novel Fluorescent Probes

Cited by 29 publications

References 19 publications

Collagen-binding Microbial Surface Components Recognizing Adhesive Matrix Molecule (MSCRAMM) of Gram-positive Bacteria Inhibit Complement Activation via the Classical Pathway

Collagen-binding Microbial Surface Components Recognizing Adhesive Matrix Molecule (MSCRAMM) of Gram-positive Bacteria Inhibit Complement Activation via the Classical Pathway

Fourier transform infrared spectroscopic imaging of cardiac tissue to detect collagen deposition after myocardial infarction

The impact of pressure overload on coronary vascular changes following myocardial infarction in rats

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