Assessment of brain dopamine levels to evaluate the role of Tylophora indicaethanolic extract on alcohol induced anxiety in Wistar albino rats

Abstract

Background: Alcohol induced health problems have tormented the world population since time immemorial. This research work was done to study the therapeutic role of Tylophora indica ethanolic extract on alcohol induced anxiety in Wistar albino rats. Method: Wistar albino rats were administered 20% alcohol, orally twice a day for 30 days. The protective role of Tylophora indica ethanolic extract was evaluated by administering it at a dose of 100 mg/kg body weight orally in alcohol treated animals for 30 days. Anxiety was assessed by elevated plus maze and light dark arena tests. After that, animals were sacrificed and brains were dissected out, to measure the levels of dopamine. To pinpoint the active constituent responsible for the anxiolytic activity, Tylophora indica ethanolic extract was subjected to HPLC-LCMS analysis. Results: Elevated plus maze and light dark arena tests showed that alcohol induced anxiety can be prevented by Tylophora indica ethanolic extract in Wistar albino rats. There was a significant increase in the levels of Dopamine in the brains of rats, which received Tylophora indica. From the HPLC-LCMS analysis report it is postulated that Tylophorinidine is the active constituent responsible for the anxiolytic activity of Tylophora indica ethanolic extract. Conclusion: Tylophora indica can mitigate the deleterious effect of alcohol in CNS.

An efficient micropropagation protocol for direct organogenesis from nodal explants of medicinal climber, Tylophora indica

“…The other reported activities include immune modulatory activity, antioxidant and free radical scavenging activity (Mohan et al, 2014, Ranemma et al, 2017, anti-infl ammatory activity (Ravikumar and Abbulu, 2011), hepatoprotective activity (Mujeeb et al, 2009), antibacterial activity (Ranemma et al, 2017), anticancer activity (Vijayakumari et al, 2014), antianxiety activity (Rao et al, 2013;Mannikoth et al, 2016), anticonvulsant activity (Hafi s et al, 2017), antiamoebic activity (Ganguly et al, 2001;Haung, 2004) and anti asthmatic activity (Umamaheshwari et al, 2017). The active constituents of Tylophora indica are alkaloids like tylophorine, tylophorinine, tylophorinidine and septidine and non-alkaloidal compounds like kaempferol, quercetin, and -amyrins, tetratriacontanol, octaosanyloctacosanoate, sigmasterol, -sitosetrol, tyloindane, wax, resin, and tannins (Rout et al, 2012;Saraswati et al, 2013;Mohan et al, 2014;Joy et al, 2017).…”

Neuroprotective Effect of Polyherbal Formulation in Parkinson’s Animal Model

“…The excitations and emission spectra were read at 330-375 nm from the spectrofluorimeter. With the addition of oxidation step's reagents in reverse order, (iodine after sodium sulfite), the tissue blanks were prepared [21].…”

Mild anxiety and depression related to elevated dopamine level

“…The BAI score has been validated and its reliability tested, and may be used to determine the anxiety level in the clinic as well as in studies. (18) The BAI questionnaire consists of 21 The depression scores are determined with the Beck Depression Inventory-II. This instrument has been proven to be of good validity and reliability and may be used to assess the depression level in populations of healthy individuals as well as in populations of individuals with disorders.…”