Assessment of biomarkers for contaminants of emerging concern on aquatic organisms downstream of a municipal wastewater discharge

Jasinska, Edyta J.; Goss, Greg G.; Gillis, Patricia L.; Kraak, Glen J. Van Der; Matsumoto, Jacqueline; Machado, Anderson Abel de Souza; Giacomin, Marina; Moon, Thomas W.; Massarsky, Andrey; Gagné, François; Servos, Mark R.; Wilson, Joanna Y.; Sultana, Tamanna; Metcalfe, Chris D.

doi:10.1016/j.scitotenv.2015.05.080

Cited by 84 publications

(29 citation statements)

References 70 publications

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“…Despite these treatment processes, reports over the past decade have indicated that WWTP effluent contain a broad range of structurally-diverse compounds which are subsequently introduced into aquatic environments (Brooks et al, 2005). These include the chemical components of common household products such as pharmaceuticals and personal care products (PPCPs), natural and synthetic hormones from contraceptive medications, alkylphenol surfactants, and perfluorinated compounds (PFCs) (Dickenson et al, 2011; Jasinska et al, 2015; Kim et al, 2012; Lubliner et al, 2010; Vidal-Dorsch et al, 2012). Compounds within the aforementioned chemical classes are termed “contaminants of emerging concern” (CECs) as there is currently little regulation of these chemicals in the environment (Diamond et al, 2011; Halden, 2015; Naidu et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

“…Finally, exposure to CECs can inhibit metabolic function and rates of growth in several fish species (Ashfield et al, 1998; Niemuth and Klaper, 2015; Yang et al, 2014), which has implications on fish populations, as body mass has been linked to survival in wild fish (Meador, 2014; Spromberg and Meador, 2005). Despite these findings, however, there are currently no established biomarkers of exposure or effect of CECs on aquatic organisms (Harding et al, 2016; Jasinska et al, 2015). …”

Section: Introductionmentioning

confidence: 99%

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Effect of contaminants of emerging concern on liver mitochondrial function in Chinook salmon

et al. 2017

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We previously reported the bioaccumulation of contaminants of emerging concern (CECs), including pharmaceuticals and personal care products (PPCPs) and perfluorinated compounds, in field-collected juvenile Chinook salmon from urban estuaries of Puget Sound, WA (Meador et al., 2016). Although the toxicological impacts of CECs on salmon are poorly understood, several of the detected contaminants disrupt mitochondrial function in other species. Here, we sought to determine whether environmental exposures to CECs are associated with hepatic mitochondrial dysfunction in juvenile Chinook. Fish were exposed in the laboratory to a dietary mixture of 16 analytes representative of the predominant CECs detected in our field study. Liver mitochondrial content was reduced in fish exposed to CECs, which occurred concomitantly with a 24–32% reduction in expression of peroxisome proliferator-activated receptor (PPAR) ϒ coactivator-1a (pgc-1α), a positive transcriptional regulator of mitochondrial biogenesis. The laboratory exposures also caused a 40–70% elevation of state 4 respiration per unit mitochondria, which drove a 29–38% reduction of efficiency of oxidative phosphorylation relative to controls. The mixture-induced elevation of respiration was associated with increased oxidative injury as evidenced by increased mitochondrial protein carbonyls, elevated expression of glutathione (GSH) peroxidase 4 (gpx4), a mitochondrial-associated GSH peroxidase that protects against lipid peroxidation, and reduction of mitochondrial GSH. Juvenile Chinook sampled in a WWTP effluent-impacted estuary with demonstrated releases of CECs showed similar trends toward reduced liver mitochondrial content and elevated respiratory activity per mitochondria (including state 3 and uncoupled respiration). Interestingly, however, respiratory control ratios were greater in fish from the contaminated site relative to fish from a minimally-polluted reference site, which may have been due to differences in the timing of exposure to CECs under laboratory and field conditions. Our results indicate that exposure to CECs can affect both mitochondrial quality and content, and support the analysis of mitochondrial function as an indicator of the sublethal effects of CECs in wild fish.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effect of contaminants of emerging concern on liver mitochondrial function in Chinook salmon

et al. 2017

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“…Generally, VTG levels in male fish are considered a biomarker for identifying exposure to EEs (Sumper & Jobling, 1995). Elevation of VTG synthesis and gene expression in the liver have been observed in males affected by EEs or other endocrine‐disrupting chemicals in wild fish, such as the cichlid, Tilapia guineensis (Adeogun, Ibor, Adeduntan, & Arukwe, ), and the fathead minnow, Pimephales promelas (Jasinska et al, ). In particular, a significant influence on VTG synthesis and gene expression by a high concentration of EEs via exposure or injection have been demonstrated in the male sand goby, Pomatoschistus minutus (Robinson et al, ), and the crucian carp, Carassius auratus (Huang et al, ), among others.…”

Section: Discussionmentioning

confidence: 99%

Standardization of plasma vitellogenin level for surveying environmental estrogen pollution using the Japanese common goby Acanthogobius flavimanus

Song

Nagae

Soyano

2018

J of Applied Toxicology

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Field surveys of the impact of environmental estrogen (EE) pollution in aquatic wildlife have been conducted using vitellogenin (VTG) as a biomarker to evaluate the influence of EE. However, a standard baseline of VTG level that can be used to evaluate EE pollution has not been fully determined. To the best of our knowledge, the present study is the first to determine the standard baseline VTG level for evaluating the biological effects of EE pollution using the Japanese common goby (Acanthogobius flavimanus) as the target model fish. Plasma VTG and estradiol-17β (E ) levels associated with the reproductive cycle of wild goby inhabiting an unpolluted environment were measured. Mean plasma VTG and E levels exhibited similar changes, increasing in the yolk vesicle stage and peaking in the tertiary yolk stage in females. However, plasma VTG and E levels showed no significant changes in males, remaining at low levels throughout the reproductive cycle. The highest VTG levels in females and males were 1.6 mg ml and 124.87 ng ml , respectively. These results indicate that the baseline level (normal level) in males was approximately 130 ng ml at most. We concluded that the threshold between normal and abnormal levels with a 10% risk rate was 150 ng ml in the wild male goby. Plasma VTG levels in males captured from Nagasaki Harbor were higher than the threshold in each reproductive developmental stage, indicating the possibility of EE pollution at this site. The biological standard baseline for VTG established in this study is useful for assessing EE pollution in natural waters.

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“…The median change of cell process of DNA replication was -1.53, and based on this value it can be concluded that this entire processes was down-regulated by 53% in 2011, suggesting a high rate of DNA mechanism activation [69,70]. In fathead minnow (Pimephales promelas) caged downstream of a wastewater outfall in the North Saskatchewan River for 4 wk there were elevated ratios of oxidized to total glutathione and an increase in the activity of antioxidant enzymes (i.e., glutathione reductase, glutathione-S-transferase) [74]. Despite repair mechanisms being activated, rainbow darter experienced difficulties in coping with nuclear DNA maintenance and its proper repair; and as a consequence, cell aging showed a 15% down-regulation.…”

Section: Cell Process Pathway Gradual Transition Over Timementioning

confidence: 94%

Returning to normal? Assessing transcriptome recovery over time in male rainbow darter (Etheostoma caeruleum) liver in response to wastewater‐treatment plant upgrades

Marjan

Martyniuk

Fuzzen

et al. 2017

Enviro Toxic and Chemistry

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The present study measured hepatic transcriptome responses in male rainbow darter (Etheostoma caeruleum) exposed to 2 municipal wastewater-treatment plants (MWWTPs; Kitchener and Waterloo) over 4 fall seasons (2011-2014) in the Grand River (Ontario, Canada). The overall goal was to determine if upgrades at the Kitchener MWWTP (in 2012) resulted in transcriptome responses indicative of improved effluent quality. The number of differentially expressed probes in fish downstream of the Kitchener outfall (904-1223) remained comparable to that downstream of Waterloo (767-3867). Noteworthy was that year and the interaction of year and site explained variability in more than twice the number of transcripts than site alone, suggesting that year and the interaction of year and site had a greater effect on the transcriptome than site alone. Gene set enrichment analysis revealed a gradual reduction in the number of gene ontologies over time at exposure sites, which corresponded with lower contaminant load. Subnetwork enrichment analysis revealed that there were noticeable shifts in the cell pathways differently expressed in the liver preupgrade and postupgrade. The dominant pathways altered preupgrade were related to genetic modifications and cell division, whereas postupgrade they were associated with the immune system, reproduction, and biochemical responses. Molecular pathways were dynamic over time, and following the upgrades, there was little evidence that gene expression profiles in fish collected from high-impact sites postupgrade were more similar to those in fish collected from reference site. Environ Toxicol Chem 2017;36:2108-2122. © 2017 SETAC.

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Assessment of biomarkers for contaminants of emerging concern on aquatic organisms downstream of a municipal wastewater discharge

Cited by 84 publications

References 70 publications

Effect of contaminants of emerging concern on liver mitochondrial function in Chinook salmon

Effect of contaminants of emerging concern on liver mitochondrial function in Chinook salmon

Standardization of plasma vitellogenin level for surveying environmental estrogen pollution using the Japanese common goby Acanthogobius flavimanus

Returning to normal? Assessing transcriptome recovery over time in male rainbow darter (Etheostoma caeruleum) liver in response to wastewater‐treatment plant upgrades

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