Assessment of biological matrices for the detection of in utero cannabis exposure

Concheiro, Marta; Gutierrez, Frank Martin; Ocampo, Alejandro; Lendoiro, Elena; Gónzalez-Colmenero, Eva; Concheiro-Guisán, Ana; Peñas-Silva, Patricia; Macias-Cortiña, Manuel; Cruz-Landeira, Angelines; López-Rivadulla, Manuel; de-Castro-Ríos, Ana

doi:10.1002/dta.3034

Cited by 6 publications

(3 citation statements)

References 45 publications

(141 reference statements)

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“…The placenta is the least investigated matrix regarding consumption of cannabinoids during pregnancy, and there is not much information regarding this matrix and cannabinoids consumption [97].…”

Section: Other Unconventional Biological Samplesmentioning

confidence: 99%

Analysis of Cannabinoids in Biological Specimens: An Update

Antunes

Barroso

Gallardo

2023

IJERPH

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Cannabinoids are still the most consumed drugs of abuse worldwide. Despite being considered less harmful to human health, particularly if compared with opiates or cocaine, cannabis consumption has important medico-legal and public health consequences. For this reason, the development and optimization of sensitive analytical methods that allow the determination of these compounds in different biological specimens is important, involving relevant efforts from laboratories. This paper will discuss cannabis consumption; toxicokinetics, the most detected compounds in biological samples; and characteristics of the latter. In addition, a comprehensive review of extraction methods and analytical tools available for cannabinoid detection in selected biological specimens will be reviewed. Important issues such as pitfalls and cut-off values will be considered.

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Section: Other Unconventional Biological Samplesmentioning

confidence: 99%

Analysis of Cannabinoids in Biological Specimens: An Update

Antunes

Barroso

Gallardo

2023

IJERPH

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“…Stability after 3 freeze/thaw cycles was evaluated in previous studies. No relevant degradation was observed for 6-MAM, morphine, cocaine, benzoylecgonine, buprenorphine, norbuprenorphine, THC, THC-COOH and 11-OH-THC (<10%), for amphetamine, metamphetamine, MDA, MDMA (<15%) and for methadone and EDDP (<20%) [7,[24][25][26][27][28][29][30].…”

Section: Applicationmentioning

confidence: 95%

Determination of Prenatal Substance Exposure Using Meconium and Orbitrap Mass Spectrometry

Hernandez

Lacroze

Doudka

et al. 2022

Toxics

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The aim of this study was to develop and to validate a toxicological untargeted screening relying on LC-HRMS in meconium including the detection of the four main classes of drugs of abuse (DoA; amphetamines, cannabinoids, opioids and cocaine). The method was then applied to 29 real samples. Analyses were performed with a liquid chromatography system coupled to a benchtop Orbitrap operating in a data-dependent analysis. The sample amount was 300 mg of meconium extracted twice by solid phase extraction following two distinct procedures. Raw data were processed using the Compound Discoverer 3.2 software (Thermo). The method was evaluated and validated on 15 compounds (6-MAM, morphine, buprenorphine, norbuprenorphine, methadone, EDDP, amphetamine, MDA, MDMA, methamphetamine, cocaine, benzoylecgonine, THC, 11-OH-THC, THC-COOH). Limits of detection were between 0.5 and 5 pg/mg and limits of identification between 5 and 50 pg/mg. Mean matrix effect was between −79 and −19% (n = 6) and mean overall recovery between 18 and 73% (n = 6) at 100 pg/mg. The application allows the detection of 88 substances, including 47 pharmaceuticals and 15 pharmaceutical metabolites, cocaine and its metabolites, THC and its metabolites, and natural (morphine, codeine) and synthetic (methadone, buprenorphine, tramadol, norfentanyl) opioids. This method is now used routinely for toxicological screening in high-risk pregnancies

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“…Recent evidence shows that umbilical cord tissue can be a convenient and appropriate substitute matrix to meconium for identifying in-utero exposure. 9 Previous research using umbilical cord tissues for ascertainment of prenatal cannabis use has used anonymous cord tissues 1 or secondary data where participants were not aware their cord tissues were tested for cannabis. 7 The aim of this study was to understand the feasibility and acceptability of collecting umbilical cord tissue for prenatal cannabis research in a community sample of pregnant persons.…”

Section: Introductionmentioning

confidence: 99%

Feasibility and acceptability of collecting umbilical cord tissue for prenatal cannabis research: A mixed-methods research study

Bayrampour,

Langlois,

Likhodi

et al. 2023

Womens Health (Lond Engl)

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Background: Large-scale longitudinal studies with biological samples are needed to examine the associations between prenatal cannabis use and birth and developmental outcomes. Objectives: The aim of this study was to understand the feasibility and acceptability of collecting umbilical cord tissue for the purpose of cannabis use testing in a community sample. Design: This is a mixed methods research study consisted of a prospective cohort study and a qualitative descriptive study. Methods: This study was conducted in Vancouver, British Columbia between January 2021 and August 2022. Participants were recruited during pregnancy, and the umbilical cord tissues were collected at birth and tested for the presence of cannabinoids. After the completion of the study, participants completed an online open-ended questionnaire about their overall experience. Data were analyzed using descriptive and thematic analyses. Results: Among the 85 eligible individuals, 57 people (67%) consented to the study. The cord tissue was collected for 39 participants (68.4%). The collection rates for participants with vaginal, elective, and emergency cesarean delivery were 73.0%, 71.4%, and 53.8%, respectively, and for those with spontaneous and induced labor were 81.5% and 50%, respectively. Four (7.0%) and seven participants (12.3%) reported prenatal cannabis use in direct and probing self-report questions, respectively. The agreement between any self-report and cord tissue test was moderate (kappa 0.53, 95% confidence interval 0.06–0.99). Qualitative findings were classified into five themes. Conclusion: The collection of cord tissue was perceived acceptable by most participants. Implementation of collection protocols for complex labors, a central hospital unit to liaise direct communications and active participants’ involvement might increase the feasibility of future studies.

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Assessment of biological matrices for the detection of in utero cannabis exposure

Cited by 6 publications

References 45 publications

Analysis of Cannabinoids in Biological Specimens: An Update

Analysis of Cannabinoids in Biological Specimens: An Update

Determination of Prenatal Substance Exposure Using Meconium and Orbitrap Mass Spectrometry

Feasibility and acceptability of collecting umbilical cord tissue for prenatal cannabis research: A mixed-methods research study

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