Assessment of biochemical and antioxidative status in patients suffering from dengue fever

Rasool, Mahmood; Malik, Arif; Khan, Kinza; Qureshi, Muhammad Saeed; Shabbir, Beenish; Zahid, Sara; Asif, Muhammad; Manan, Abdul; Rashid, Sana; Khan, Shirajul Islam; Arsalan, Hafiz Muhammad; Alam, Rabail; Arooj, Mahwish; Qazi, Mahmood Husain; Chaudhary, Adeel G.; Abuzenadah, Adel M.; Al‐Qahtani, Mohammed; Karim, Sajjad

doi:10.1007/s11596-015-1446-x

Cited by 5 publications

(8 citation statements)

References 33 publications

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“…Al-Alimi et al [38] reported that deficiency of glucose 6 phosphate dehydrogenase enzyme which required for maintaining glutathione in the reduced state leads to the production of nitric oxide, superoxide radicles and viral persistence in DENV-2 infected monocytes. Our study results were in agreement with observation on earlier reports on antioxidants levels which were diminished in dengue study subjects [19,20]. The study subjects belong to Puducherry and Tamilnadu region which were reported for the prevalence of all four dengue serotypes earlier [39].…”

Section: Discussionsupporting

confidence: 93%

“…Besides the study undergoes some of the limitations like a discrete data analysis was not performed for juvenile or adult cohort because of the limited number of pediatric dengue cases. Because of the limited sample volume, we could not able to study the levels of protein in the circulation, even though the study corroborates the earlier reports of disrupted antioxidant defence in dengue patients [19,20].…”

Section: Discussionsupporting

confidence: 79%

“…Antioxidant enzymes like superoxide dismutase, catalase, and glutathione peroxidase come into the action synergistically to eradicate the free radicals [17,18]. Earlier studies reported a lower level of antioxidant markers such as CAT, SOD, GPx, reduced glutathione (GSH) and paraoxonase in the peripheral circulation of dengue study subjects [19,20].…”

Section: Introductionmentioning

confidence: 99%

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A Study on Gene Expression Profile of Endogenous Antioxidant Enzymes: CAT, MnSOD and GPx in Dengue Patients

et al. 2017

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Dengue is an arthropod-borne threat among tropical countries. Currently no effective means to treat the virus or to predict which patient will develop the severe form of the disease. Recently the relationship between oxidative/antioxidative response and dengue pathogenesis was suggested. Based on this the present study has analysed the expression of endogenous antioxidant genes: Catalase (CAT), Superoxide dismutase (MnSOD) and Glutathione peroxidase in patients with dengue compared to other febrile illness (OFI) and healthy controls. The study enrolled 88 dengue confirmed patients comprising 56 were patients with non-severe dengue, and 32 were severe dengue cases, 31 were patients with OFI, and 63 healthy controls were also involved. Peripheral blood mononuclear cells isolated from patients and controls during the day of admission and from the available cases on the day of defervescence were used to estimate the transcript levels by quantitative PCR. The expression levels of all the three genes were found to be down-regulated throughout the course of dengue infection ( < 0.05) and OFI cases compared to healthy controls. Within dengue group, no significant difference was observed in any of the parameters between severe and non-severe cases. Interestingly, a significant down-regulation of MnSOD expression was recorded in secondary dengue infection compared to primary during admission ( < 0.05). It was found that all the down-regulated study genes have positively correlated in all dengue cases during the day of admission ( < 0.01). But during defervescence, the same was found only between CAT and MnSOD. Down-regulated endogenous antioxidant enzymes during dengue infection could be the possible rationale of oxidative stress reported in dengue disease earlier. The present study markers could not distinguish dengue from OFI cases and severe from non-severe dengue cases. Mechanism of down-regulation has to be explored further which will pave the way for the therapeutic target in dengue disease.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 79%

Section: Introductionmentioning

confidence: 99%

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A Study on Gene Expression Profile of Endogenous Antioxidant Enzymes: CAT, MnSOD and GPx in Dengue Patients

et al. 2017

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“…In DENV-infected patients, the level of oxidative stress was found to be maximally increased in DSS, followed by DHF, and minimal in dengue fever (DF) (16). However, there is no clear explanation as to how DENV infection induces oxidative stress, although previous studies have reported lower levels of antioxidant markers such as catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx), reduced glutathione (GSH), and paraoxonase in the peripheral circulation of dengue study subjects (17,18). Thus, one possible mechanism of oxidative stress could be the downregulation of endogenous antioxidant enzymes during dengue infection.…”

mentioning

confidence: 99%

Dengue Virus Targets Nrf2 for NS2B3-Mediated Degradation Leading to Enhanced Oxidative Stress and Viral Replication

Ferrari

Zevini

Palermo

et al. 2020

J Virol

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Dengue virus (DENV) is a mosquito-borne virus that infects upward of 300 million people annually and has the potential to cause fatal hemorrhagic fever and shock. While the parameters contributing to dengue immunopathogenesis remain unclear, the collapse of redox homeostasis and the damage induced by oxidative stress have been correlated with the development of inflammation and progression towards the more severe forms of disease. In the present study, we demonstrate that the accumulation of reactive oxygen species (ROS) late after DENV infection (>24 hpi) resulted from a disruption in the balance between oxidative stress and the Nuclear factor erythroid 2-related factor 2 (Nrf2)-dependent antioxidant response. The DENV NS2B3 protease complex strategically targeted Nrf2 for degradation in a proteolytic-independent manner; rather NS2B3 licensed Nrf2 for lysosomal degradation. Impairment of the Nrf2 regulator by the NS2B3 complex inhibited the antioxidant gene network and contributed to the progressive increase in ROS levels, along with an increased virus replication and inflammatory or apoptotic gene expression. By 24hpi, when increased levels of ROS and antiviral proteins were observed, it appeared that the pro-viral effect of ROS overcame the antiviral effects of the IFN response. Overall, these studies demonstrate that DENV infection disrupts the regulatory interplay between DENV-induced stress responses, Nrf2 anti-oxidant signaling and the host antiviral immune response, thus exacerbating oxidative stress and inflammation in DENV infection. IMPORTANCE Dengue virus (DENV) is a mosquito-borne pathogen that threatens 2.5 billion people in over 100 countries annually. Dengue infection induces a spectrum of clinical symptoms, ranging from classical dengue fever to severe dengue hemorrhagic fever or dengue shock syndrome; however, the complexities of DENV immunopathogenesis remain controversial. Previous studies reported the importance of the transcription factor Nrf2 in the control of the redox homeostasis and antiviral/inflammatory or death responses to DENV. Importantly, production of reactive oxygen species and the subsequent stress response have been linked to the development of inflammation and progression towards the more severe forms of the disease. Here, we demonstrate that DENV uses the NS2B3 protease complex to strategically target Nrf2 for degradation, leading to a progressive increase in oxidative stress, inflammation and cell death in infected cells. This study underlines the pivotal role of the Nrf2 regulatory network in the context of DENV infection.

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“…5) The dengue infection could affect the antioxidant enzymatic-like SOD, CAT, and GSH. However, the exact mechanism on the matter remains unclear, but the previous study reported that SOD, CAT, and antioxidative serum capacity was found lower in dengue fever patients than control [21].…”

Section: Resultsmentioning

confidence: 95%

A Different Approach to Assess Oxidative Stress in Dengue Hemorrhagic Fever Patients Through The Calculation of Oxidative Stress Index

Hartoyo

Thalib²,

Sari³

et al. 2017

J.Trop.Life.Science

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The objectives of this study were to determine the involvement of Oxidative Stress (OS) in the pathogenesis of dengue hemorrhagic fever (DHF) through the analysis of oxidative stress Index (OSI). The levels of malondialdehyde (MDA), superoxide dismutase (SOD) and catalase (CAT) activity, and OSI were measured in 61 child dengue patients and (aged 6 months-18 years) with three different stages of DHF, i.e stage I, II, and III. The results show that the levels of MDA, SOD and CAT activity, and OSI significantly different between the group. The all parameters that investigated in this present study seems higher MDA level and OSI in the higher grade of DHF, except for SOD and CAT activity. From this result, it can be concluded that oxidative stress pathways might be involved in the pathomechanism of DHF and OSI might be used as a biomarker for OS and the severity in DHF patients.

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Assessment of biochemical and antioxidative status in patients suffering from dengue fever

Cited by 5 publications

References 33 publications

A Study on Gene Expression Profile of Endogenous Antioxidant Enzymes: CAT, MnSOD and GPx in Dengue Patients

A Study on Gene Expression Profile of Endogenous Antioxidant Enzymes: CAT, MnSOD and GPx in Dengue Patients

Dengue Virus Targets Nrf2 for NS2B3-Mediated Degradation Leading to Enhanced Oxidative Stress and Viral Replication

A Different Approach to Assess Oxidative Stress in Dengue Hemorrhagic Fever Patients Through The Calculation of Oxidative Stress Index

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