Assessment of bacterial endosymbionts and the host, <i>Bemisia tabaci</i> (Hemiptera: Aleyrodidae), using rRNA and mitochondrial cytochrome oxidase I gene sequences

Hashmi, Tahseen Raza; Devi, Salam Rita; Meshram, Naresh M.; Prasad, Ram

doi:10.1080/19420889.2018.1433442

Cited by 11 publications

(7 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Several Arsenophonus OTUs have been reported from Asia II samples in India (Singh et al, 2012), Q-eastern clade mitotypes in Israel, "Ms mitotype" endemic to Uganda and Reunion Island in the Indian Ocean, the presumed southward limit of its range (Gueguen et al, 2010;Zchori-Fein et al, 2014). Studies have documented between four to five Arsenophonus OTUs associated with B. tabaci (Gueguen et al, 2010;Hashmi, Devi, Meshram, & Prasad, 2018;Kanakala & Ghanim, 2019). However, whether these OTUs are "species" of Arsenophonus or represent multiple copies of the same 16S rRNA gene in the same individual requires additional investigation.…”

Section: Arsenophonus Otusmentioning

confidence: 99%

Genetic variability, community structure, and horizontal transfer of endosymbionts among three Asia II‐Bemisia tabaci mitotypes in Pakistan

Paredes‐Montero

Zia-ur-Rehman

Hameed

et al. 2020

Ecology and Evolution

View full text Add to dashboard Cite

Endosymbionts associated with the whitefly Bemisia tabaci cryptic species are known to contribute to host fitness and environmental adaptation. The genetic diversity and population complexity were investigated for endosymbiont communities of B. tabaci occupying different micro‐environments in Pakistan. Mitotypes of B. tabaci were identified by comparative sequence analysis of the mitochondria cytochrome oxidase I (mtCOI) gene sequence. Whitefly mitotypes belonged to the Asia II‐1, ‐5, and ‐7 mitotypes of the Asia II major clade. The whitefly–endosymbiont communities were characterized based on 16S ribosomal RNA operational taxonomic unit (OTU) assignments, resulting in 43 OTUs. Most of the OTUs occurred in the Asia II‐1 and II‐7 mitotypes (r2 = .9, p < .005), while the Asia II‐5 microbiome was less complex. The microbiome OTU groups were mitotype‐specific, clustering with a basis in phylogeographical distribution and the corresponding ecological niche of their whitefly host, suggesting mitotype‐microbiome co‐adaptation. The primary endosymbiont Portiera was represented by a single, highly homologous OTU (0%–0.67% divergence). Two of six Arsenophonus OTUs were uniquely associated with Asia II‐5 and ‐7, and one occurred exclusively in Asia II‐1, two only in Asia II‐5, and one in both Asia II‐1 and ‐7. Four other secondary endosymbionts, Cardinium, Hemipteriphilus, Rickettsia, and Wolbachia OTUs, were found at ≤29% frequencies. The most prevalent Arsenophonus OTU was found in all three Asia II mitotypes (55% frequency), whereas the same strain of Cardinium and Wolbachia was found in both Asia II‐1 and ‐5, and a single Hemipteriphilus OTU occurred in Asia II‐1 and ‐7. This pattern is indicative of horizontal transfer, suggestive of a proximity between mitotypes sufficient for gene flow at overlapping mitotype ecological niches.

show abstract

Section: Arsenophonus Otusmentioning

confidence: 99%

Genetic variability, community structure, and horizontal transfer of endosymbionts among three Asia II‐Bemisia tabaci mitotypes in Pakistan

Paredes‐Montero

Zia-ur-Rehman

Hameed

et al. 2020

Ecology and Evolution

View full text Add to dashboard Cite

show abstract

“…But contradictory to our finding of the presence of Asia-II-1 in Pantnagar, they have reported Asia-II-7 to be the predominant cryptic species in the Pantnagar. The presence of the Asia-II-1 in soybean has been reported in New Delhi (Hashmi et al 2018), Uttar Pradesh and Punjab (Ellango et al 2015). The presence of Asia 1 and Asia-II-1 in different crops in the country is also evident from the findings of Chowda-Reddy et al (2012), Roopa et al (2015) and Prabhulinga et al (2021).…”

Section: Genetic Group Analysismentioning

confidence: 79%

“…Since the morphological characteristics cannot be fully depended upon for differentiating the different genetic groups, scientists prefer the use of PCR amplification and sequencing of the conserved region in the mitochondrial cytochrome oxidase I (mtCO1) gene (Frohlich et al, 1999). Among the 34 putative species of B. tabaci reported globally, nine have been recognized in India (Hashmi et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Genetic Diversity and Geographic Distribution of Bemisia tabaci Species Complex Infecting Soybean in Northern Hills and North Western Plains of India

Aravind¹,

Singh²,

Bhatt³

et al. 2022

View full text Add to dashboard Cite

Background: The Silverleaf whitefly, Bemisia tabaci (Gennadius), is a pest of global significance causing huge economic loss to a wide range of agricultural crops. The pest is regarded as a cryptic species complex which differs in its adaptability to host, fecundity and resistance to insecticides. The expected outcome of the investigation is updated information on prevalence of cryptic species of B. tabaci in the major soybean growing areas in the region. Methods: A roving survey was carried out to assess the occurrence of B. tabaci in the major soybean growing areas of Northern Hills and North Western Plain zones during three consecutive crop seasons from 2018 to 2020. The survey locations were divided in to two broad groups viz., Hills and Plains (Tarai region) and a total of 76 villages were visited during the survey. Cryptic species composition was analyzed using the mtCOI sequence through PCR amplification. Result: The mean whitefly population per plant for the three years in hills and plains were 0.53 and 3.55, respectively. The highest whitefly population was observed in 2019 (1.79 per plant) followed by 2018 (1.50 per plant) and 2020 (1.11 per plant). There was significant (p less than 0.0001) negative correlation (r= -0.749) with altitude. Phylogenetic relationships indicated the existence of two cryptic species Asia-1 and Asia-II-1. Of these, Asia-II-1 was found to be prominent especially in the North Western Plain Zone. In the Northern Hill Zone both Asia 1 and Asia-II-1 were detected.

show abstract

“…It has been discovered that the P-endosymbionts Portiera aleyrodidarum and S-endosymbionts such as Arsenophonus , Cardinium , Fritschea , Hamiltonella , Rickettsia , and Wolbachia were infected in whiteflies ( Bing et al, 2013 ; Chiel et al, 2007 ; Chu et al, 2011 ; Everett et al, 2005 ; Karut et al, 2017 ; Thao & Baumann, 2004 ; Zchori-Fein, Lahav & Freilich, 2014 ). Previous studies have investigated the prevalence, diversity and evolution of endosymbionts in the B. tabaci species complex from different countries or regions (e.g., Turkey, China, Brazil, and Africa) ( Ahmed et al, 2010 ; Bing et al, 2013 , 2014 ; Ghosh, Bouvaine & Maruthi, 2015 ; Hashmi et al, 2018 ; Jahan et al, 2015 ; Karut et al, 2017 ; Marubayashi et al, 2014 ; Santos-Garcia et al, 2015 ; Sseruwagi et al, 2018 ; Thierry et al, 2011 , 2015 ). However, most of these reports only focused on the two invasive cryptic species MEAM1 and MED; furthermore, for studies from China, the sample size and distribution range were limited.…”

Section: Introductionmentioning

confidence: 99%

Diversity and evolution of the endosymbionts of Bemisia tabaci in China

Tang

Cai

Yuan³

et al. 2018

PeerJ

View full text Add to dashboard Cite

The whitefly Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) is a cryptic species complex, including members that are pests of global importance. This study presents a screening of B. tabaci species in China for infection by the primary endosymbiont, Portiera aleyrodidarum, and two secondary endosymbionts, Arsenophonus and Cardinium. The results showed that P. aleyrodidarum was detected in all B. tabaci individuals, while Arsenophonus was abundant in indigenous species of B. tabaci Asia II 1, Asia II 3, and China 1 but absent in the invasive species, Middle East-Asia Minor 1 (MEAM1); Cardinium presented in the Mediterranean (MED), Asia II 1 and Asia II 3 species but was rarely detected in the MEAM1 and China 1 species. Moreover, phylogenetic analyses revealed that the P. aleyrodidarum and mitochondrial cytochrome oxidase 1 (mtCO1) phylograms were similar and corresponding with the five distinct cryptic species clades to some extent, probably indicating an ancient infection followed by vertical transmission and subsequent co-evolutionary diversification. In contrast, the phylogenetic trees of Arsenophonus and Cardinium were incongruent with the mtCO1 phylogram, potentially indicating horizontal transmission in B. tabaci cryptic species complex. Taken together, our study showed the distinct infection status of endosymbionts in invasive and indigenous whiteflies; we also most likely indicated the co-evolution of primary endosymbiont and its host as well as the potential horizontal transfer of secondary endosymbionts.

show abstract

Assessment of bacterial endosymbionts and the host, Bemisia tabaci (Hemiptera: Aleyrodidae), using rRNA and mitochondrial cytochrome oxidase I gene sequences

Cited by 11 publications

References 46 publications

Genetic variability, community structure, and horizontal transfer of endosymbionts among three Asia II‐Bemisia tabaci mitotypes in Pakistan

Genetic variability, community structure, and horizontal transfer of endosymbionts among three Asia II‐Bemisia tabaci mitotypes in Pakistan

Genetic Diversity and Geographic Distribution of Bemisia tabaci Species Complex Infecting Soybean in Northern Hills and North Western Plains of India

Diversity and evolution of the endosymbionts of Bemisia tabaci in China

Contact Info

Product

Resources

About