Assessment of avoidance behaviors in mouse models of muscle pain

Pratt, Dane; Fuchs, Perry N.; Sluka, Kathleen A.

doi:10.1016/j.neuroscience.2013.05.058

Cited by 28 publications

(37 citation statements)

References 37 publications

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“…3B). We further tested the contribution of TRPV1 to bite force changes induced by masseter injection of carrageenan, which also causes muscle inflammation 47 . Knockout of TRPV1 showed only modestly decreased bite force reduction 3 hours following carrageenan injection, which did not reach statistical significance (Fig.…”

Section: Resultsmentioning

confidence: 99%

Spontaneous and Bite-Evoked Muscle Pain Are Mediated by a Common Nociceptive Pathway With Differential Contribution by TRPV1

Wang

Lim

Joseph

et al. 2017

The Journal of Pain

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Spontaneous pain and function-associated pain are prevalent symptoms of multiple acute and chronic muscle pathologies. We established mouse models for evaluating spontaneous pain and bite-evoked pain from masseter muscle, and determined the roles of TRPV1 and the contribution of TRPV1- or NK1-dependent nociceptive pathways. Masseter muscle inflammation increased mouse grimace scale (MGS) scores and face wiping behavior which were attenuated by pharmacological or genetic inhibition of TRPV1. Masseter inflammation led to a significant reduction in bite force. Inhibition of TRPV1 only marginally relieved the inflammation-induced reduction of bite force. These results suggest differential extent of contribution of TRPV1 to the two types of muscle pain. However, chemical ablation of TRPV1-expressing nociceptors or chemogenetic silencing of TRPV1-lineage nerve terminals in masseter muscle attenuated inflammation-induced changes in both MGS scores and bite force. Furthermore, ablation of neurons expressing neurokinin 1 (NK1) receptor in trigeminal subnucleus caudalis also prevented both types of muscle pain. Our results suggest that TRPV1 differentially contribute to spontaneous pain and bite-evoked muscle pain, but TRPV1-expressing afferents and NK1-expressing second order neurons commonly mediate both types of muscle pain. Therefore, manipulation of the nociceptive circuit may provide a novel approach for management of acute or chronic craniofacial muscle pain.

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Section: Resultsmentioning

confidence: 99%

Spontaneous and Bite-Evoked Muscle Pain Are Mediated by a Common Nociceptive Pathway With Differential Contribution by TRPV1

Wang

Lim

Joseph

et al. 2017

The Journal of Pain

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“…Additionally, many behavioral assays utilize the hindpaw in order to assess phenotypes such as pain, motor function, weight-bearing, locomotion, and exploratory behaviors (Gregory et al 2013; Pratt et al 2013; Pertici et al 2014; Freund et al 2015). For these reasons, a sustained peripheral drug delivery system is required in order to accurately study the physiology and behavior of neurological diseases in a mouse model.…”

Section: Discussionmentioning

confidence: 99%

Novel intrathecal and subcutaneous catheter delivery systems in the mouse

Oladosu

Ciszek

O'Buckley

et al. 2016

Journal of Neuroscience Methods

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Background Catheter systems that permit targeted delivery of genes, molecules, ligands, and other agents represent an investigative tool critical to the development of clinically relevant animal models that facilitate the study of neurological health and disease. The development of new sustained catheter delivery systems to spinal and peripheral sites will reduce the need for repeated injections, while ensuring constant levels of drug in plasma and tissues. New Method Here, we introduce two novel catheter delivery systems in the mouse: the O’Buckley intrathecal catheter system for sustained delivery to the spinal region and a subcutaneous bifurcated catheter system for sustained drug delivery to both hindpaws. Results The O’Buckley intrathecal catheter system consistently distributed Evans Blue throughout the spinal cord, with the greatest concentration at the thoracic region, and with an 85% surgery success rate. The subcutaneous catheter system consistently distributed Evans Blue to the hindlimbs, with a 100% surgery success rate. Comparison to existing method The O’Buckley intrathecal catheter system accomplishes sustained drug delivery to the spinal region, with a 2-fold increase in surgery success rate, as compared to the traditional method. Our subcutaneous bifurcated catheter system accomplishes sustained drug delivery to both hindpaws, eliminating the need for repeated intraplantar injections. Conclusions We have developed catheter systems that improve upon traditional methods in order to achieve sustained localized drug delivery to spinal tissues and to hindpaw tissues surrounding peripheral sciatic nerve terminals. These methods have a broad reach, and can be used to enhance behavioral, physiologic and mechanistic studies in mice.

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“…To assess supraspinal processing of nociception, we used the escape/avoidance test as previously published in mice [24]. The testing box was made of Plexiglas (16 cm×7 cm×13 cm) with two chambers and placed on top of a wire mesh screen.…”

Section: Methodsmentioning

confidence: 99%

IL-10 Cytokine Released from M2 Macrophages Is Crucial for Analgesic and Anti-inflammatory Effects of Acupuncture in a Model of Inflammatory Muscle Pain

et al. 2014

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Muscle pain is a common medical problem that is difficult to treat. One nonpharmacological treatment used is acupuncture, a procedure in which fine needles are inserted into body points with the intent of relieving pain and other symptoms. Here we investigated the effects of manual acu-puncture (MA) on modulating macrophage phenotype and interleukin-10 (IL-10) concentrations in animals with muscle inflammation. Carrageenan, injected in the gastrocnemius muscle of mice, induces an inflammatory response characterized by mechanical hyperalgesia and edema. The inflammation is initially a neutrophilic infiltration that converts to a macrophage-dominated inflammation by 48 h. MA of the Sanyinjiao or Spleen 6 (SP6) acupoint reduces nociceptive behaviors, heat, and mechanical hyperalgesia and enhanced escape/avoidance and the accompanying edema. SP6 MA increased muscle IL-10 levels and was ineffective in reducing pain behaviors and edema in IL-10 knockout (IL-10−/−) mice. Repeated daily treatments with SP6 MA induced a phenotypic switch of muscle macrophages with reduced M1 macrophages (pro-inflammatory cells) and an increase of M2 macrophages (anti-inflammatory cells and important IL-10 source). These findings provide new evidence that MA produces a phenotypic switch in macrophages and increases IL-10 concentrations in muscle to reduce pain and inflammation.

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Assessment of avoidance behaviors in mouse models of muscle pain

Cited by 28 publications

References 37 publications

Spontaneous and Bite-Evoked Muscle Pain Are Mediated by a Common Nociceptive Pathway With Differential Contribution by TRPV1

Spontaneous and Bite-Evoked Muscle Pain Are Mediated by a Common Nociceptive Pathway With Differential Contribution by TRPV1

Novel intrathecal and subcutaneous catheter delivery systems in the mouse

IL-10 Cytokine Released from M2 Macrophages Is Crucial for Analgesic and Anti-inflammatory Effects of Acupuncture in a Model of Inflammatory Muscle Pain

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