Assessment of Antiplasmodial and Anti-anaemic Activities of Hoslundia opposita, an Ivorian Medicinal Plant

Moustapha, Karamoko; Karim, Tuo; Offianan, Touré André; Beourou, Sylvain; David, A. H.; Albert, Gnondjui; Stéphane, Yao; Philippe, Bidie Alain Dit

doi:10.9734/jamb/2018/41897

Cited by 4 publications

(2 citation statements)

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“…The development of new antimalarials from highly active natural products is crucial in order to overcome the increasing resistance of Plasmodium to malarial drugs (Bero, 2009;Akin-Osanaiye et al, 2013;Moustapha et al, 2018). Historically, medicinal plants have served as sources of new pharmaceutical products like quinine and artemisinin (Newman et al, 2000;Koehn, 2005) and inexpensive starting materials for synthesising many known drugs.…”

Section: Introductionmentioning

confidence: 99%

Synergy and Antagonism in Antimalarial Crude Extract Combinations

Kidima,

Wambura,

Nyigo

et al. 2024

Tanzania J Hlth Res

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Background Malaria accounts for around 4.8% of all recorded fatalities in Tanzania. Medicinal plants such as Caesalpinia bonducella, Azadirachta indica, and Annickia kummeriae have demonstrated promise in treating many diseases, including malaria. However, their combined activity against malaria has not been documented. Combination therapy using some medicinal plants with antimalarial activities may enhance safety and efficacy and reduce the evolution of parasite resistance. Objectives This study aimed to investigate antiplasmodium activities of different combinations of crude extracts from selected medicinal plants. Azadirachta indica leaves, Annickia kummeriae and Caesalpinia bonducella were extracted using dichloromethane (DCM). Methods An in vivo acute toxicity study of both individual and combined crude extracts was carried out according to Chinedu et al., 2015. The in vivo antiplasmodial activity of individual and combined crude extracts was performed in mice inoculated with Plasmodium berghei (ANKA strain) using Peters’s 4-day suppressive test. Results Individually, Caesalpinia bonducella crude extracts exhibited the highest in vivo antiplasmodial efficacy (91% parasite suppression) than A. kummeriae (73% parasite suppression) and A. indica (60% parasite suppression) at 800 mg/kg/day. The A. indica and A. summarize combinations and A. indica and C. bonducella demonstrated higher antiplasmodial activity (synergism-combination index 0.29 and 0.97, respectively) than their constituents. However, combining A. kummeriae and C. bonducella produced the lowest antiplasmodial activity (antagonism- combination index 40.67) than its extracts. The high antiplasmodial potencies (ED50) demonstrated by AiAk and AiCb are significant and critical results for traditional, complementary and alternative medicine. Conclusion Therefore, these preliminary findings suggest that AiAk and AiCb are potential antiplasmodium herbal therapies. Further research should be undertaken to investigate the antiplasmodium effect of AiAk and AiCb in humans.

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Section: Introductionmentioning

confidence: 99%

Synergy and Antagonism in Antimalarial Crude Extract Combinations

Kidima,

Wambura,

Nyigo

et al. 2024

Tanzania J Hlth Res

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“…In Uganda, it is locally named kamunye (in central region) and is used for cleansing the uterus after birth, treat vaginal laceration, wounds, and other diseases [15]. Research has shown evidence that the plant has potential for wound healing, central nervous depression, possesses antibacterial, antioxidant, hepatoprotective, HIV inhibition, insecticidal [14], anti-anaemic [16] and anticancer properties [17]. The antioxidant activity of this plant from different countries has been investigated forexample, Nigeria [18], Tanzania, Mozambique, Ghana [14], as well as Zimbabwe [19].…”

Section: Introductionmentioning

confidence: 99%

Hoslundia opposita vahl; a potential source of bioactive compounds with antioxidant and antibiofilm activity for wound healing

Namuga,

Muwonge,

Nasifu

et al. 2024

BMC Complement Med Ther

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Background Biofilms and oxidative stress retard wound healing. The resistance of biofilms to antibiotics has led to a search for alternative approaches in biofilm elimination. Antioxidants work synergistically with antibacterial agents against biofilms. Hence recent research has suggested plants as candidates in the development of new alternatives in biofilm treatments and as antioxidants due to the presence of phytocompounds which are responsible for their bioactivities. Hoslundia opposita Vahl is one of the plants used by traditional healers to treat wounds and other infections, this makes it a potential candidate for drug discovery hence, in this study, we investigate the antibiofilm and antioxidant activity of methanolic extract of hoslundia opposita Vahl from Uganda. We also identify phytochemicals responsible for its bioactivity. Method the plant was extracted by maceration using methanol, and the extract was investigated for antioxidant activity using 2,2-diphenyl-1-picrylhydrazyl radical (DPPH) assay. The antibiofilm activity using microtiter plate assay (MTP) assay where the Minimum biofilm inhibitory concentration required to inhibit 50% or 90% of the biofilm (MBIC50 and MBIC90) and Minimum biofilm eradication concentration required to remove 50% or 90% of the biofilm (MBEC50 and MBEC90) were measured. It was further analysed for its phytochemical composition using quantitative screening, as well as Gas chromatography-mass spectrometry (GC-MS) and Liquid chromatography mass-spectrometry (LC-MS). Results H. Opposita Vahl extract showed good antioxidant activity with of 249.6 mg/mL. It inhibited the growth of P. aeruginosa and S. aureus biofilms with MBIC50 of 28.37 mg/mL and 10 mg/mL, respectively. It showed the ability to eradicate P. aeruginosa and S. aureus biofilms with MBEC50 of 23.85 and 39.01 mg/mL respectively. Phytochemical analysis revealed the presence of alkaloids, tannins, flavonoids, and phenols. GC-MS analysis revealed 122 compounds in the extract of which, 23 have evidence of antioxidant or antibiofilm activity in literature. The most abundant compounds were; 1,4- Citric acid, Tetracontane-1,40-diol (43.43.3%, 1, Olean-12-en-28-oic acid, 3-hydroxy-, methyl ester, (3.beta) (15.36%) 9-Octadecenamide (12.50%), Squalene (11.85%) Palmitic Acid 4TMS (11.28%), and alpha Amyrin (11.27%). The LC-MS identified 115 and 57 compounds in multiple reaction mode (MRM) and scan modes respectively. Conclusion H. opposita Vahl showed antibiofilm and antioxidant activity due to bioactive compounds identified, hence the study justifies its use for wound healing. It can be utilised in further development of new drugs as antibiofilm and antioxidants.

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A review of efficacy and safety of Ugandan anti-malarial plants with application of RITAM score

Angupale

Tusiimire

Ngwuluka

2023

Malar J

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Background Malaria, a treatable disease mainly caused by Plasmodium falciparum has remained a health challenge in Africa, a continent that accounted for 96% of total global cases and deaths in 2021. Uganda, a malaria endemic country is experiencing malaria parasite resistance to some of the drugs used in the artemisinin-based combination therapy (ACT). In an effort to prioritize herbal medicines for new product development, this review synthesized the available safety and efficacy literature on the Ugandan anti-malarial plants to suggest most effective herbal plants. Methods Literature was exhaustively searched using engines and databases, such as Google scholar, Pubmed, and Scopus-indexed journals during the period of June 2020–December 2021. In the first phase, information on ethnobotanical uses of anti-malarial plants in Uganda was gathered and synthetized to generate a list of plants, followed by data on anti-malarial efficacy (both in vitro and in vivo) on each listed plant. Minimum inhibitory concentrations (µg/ml), and % parasite suppression for every plant were scored using The Research Initiative on Traditional and Antimalarial Methods (RITAM) scoring system. The best twenty (20) plants were evaluated for acute safety (LD50) data in rat model, plant parts used, ease of cultivation, presence of clinical studies and other relevant factors for suggesting the best three (3) plants for future anti-malarial product development. Results Over one hundred twenty-six (126) plant species are used in Uganda for treatment of malaria in local communities. Out of these, about 33% (41) have been studied for efficacy and safety, with Artemisia annua and Vernonia amygdalina being the most extensively studied and among the best twenty (20) anti-malarial plants in Uganda. Both are limited by parasite recrudescence in clinical studies. Microglossa pyrifolia, a very potent plant (IC50 = 0.03 – 0.05 µg/ml has potential to penetrate the liver and could ameliorate the challenge of recrudescence if combined with A. annua and V. amygdalina in a polyherbal formulation. Conclusion There are many plants with promising potential for malaria treatment in Uganda and a herbal combination of A. annua, V. amydalina and M. pyrifolia could offer the next herbal ACT if carefully studied and developed.

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Assessment of Antiplasmodial and Anti-anaemic Activities of Hoslundia opposita, an Ivorian Medicinal Plant

Cited by 4 publications

References 0 publications

Synergy and Antagonism in Antimalarial Crude Extract Combinations

Synergy and Antagonism in Antimalarial Crude Extract Combinations

Hoslundia opposita vahl; a potential source of bioactive compounds with antioxidant and antibiofilm activity for wound healing

A review of efficacy and safety of Ugandan anti-malarial plants with application of RITAM score

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