Assessment of Antioxidative and Alpha-Amylase Potential of Polyherbal Extract

Patwekar, Mohsina; Patwekar, Faheem; Mezni, Amine; Sanaullah, Syed; Fatema, Shaikh Rohin; Almas, Ustad; Ahmad, Irfan; Tirth, Vineet; Mallick, Jewel

doi:10.1155/2022/7153526

Cited by 13 publications

(8 citation statements)

References 26 publications

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“…These are the steps: vancomycin and AgNP surface interaction. Ag + ions or AgNPs are released by a complex that is bounded with the bacteria [ 16 ]. AgNPs become poisonous when they bind to the phosphorous in DNA and the sulfur in proteins.…”

Section: Resources and Procedures For Experimentsmentioning

confidence: 99%

Vancomycin as an Antibacterial Agent Capped with Silver Nanoparticles: An Experimental Potential Analysis

Patwekar

Alghamdi

et al. 2022

BioMed Research International

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For the treatment of various infections, a variety of antimicrobial drugs are formulated. Nevertheless, many bacterial infections now exhibit antibiotic resistance due to the widespread utilization antibiotics. Methicillin-resistant among the most dangerous multidrug-resistant bacteria is Staphylococcus aureus (MRSA). Vancomycin became a viable therapy option due to MRSA resistance to methicillin medicines. One of the well-informed antibacterial compounds with wideband antibacterial activity is silver nanoparticles (AgNPs). AgNPs are thus suitable candidates for usage in conjunction alongside vancomycin to increase its antibacterial effect. The goal of the present research work is to boost the antibacterial potency of the glycopeptide antibiotic vancomycin towards Gram-positive (Staphylococcus aureus) but also Gram-negative (Escherichia coli) bacteria. The chemical reduction approach is used to create a colloidal solution of silver nanoparticles utilizing silver nitrate as a precursor in the environment of the ionic surfactant trisodium citrate that serves as covering including reducing reagent. Vancomycin was used to functionalize the synthesized nanoparticles and create the nanodrug complex (Van@AgNPs). The synergistic antibacterial potential of silver nanoparticles coated with vancomycin on both test pathogens was investigated using the agar well diffusion technique. The antibacterial potency for both classes of bacteria has significantly increased, according to the well diffusion test. It has been noted that this improvement is synergistic instead of additive.

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Section: Resources and Procedures For Experimentsmentioning

confidence: 99%

Vancomycin as an Antibacterial Agent Capped with Silver Nanoparticles: An Experimental Potential Analysis

Patwekar

Alghamdi

et al. 2022

BioMed Research International

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“…Protein interaction network analysis and KEGG enrichment analysis showed that the functional proteins corresponding to the fve genes CDK2, E2F1, CDKN1A, CDK1, and CCND1 and CCNB1 may be the key proteins for the compound to play the role of hypoglycemic; the KEGG enrichment analysis obtained seven signal pathways: protein domain specifc binding, protein homodimerization activity, protein kinase activity, DNA-binding transcription factor binding, ligase activity, cyclin-dependent protein serineL-amino acid transmembrane transporter activity, calcium ion binding, and amino acid binding. At present, the compound drug researched by Quazi et al has proved that the extract possesses numerous classes of chemicals such as alkaloids, glycosides, tannins, polyphenols, and terpenoids, which can contribute to the antidiabetic activity through alpha-amylase inhibition [12,13].…”

Section: Discussionmentioning

confidence: 99%

To Investigate the Potential Mechanism of Huanglian Jiangtang Formula Lowering Blood Sugar in View of Network Pharmacology and Molecular Docking Technology

Li¹,

Zhou

Lü

et al. 2023

Evidence-Based Complementary and Alternative Medicine

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Objective. In view of network pharmacology and molecular docking technology, to explore the targets as well as effect mechanism of the Huanglian Jiangtang formula (including Coptis chinensis, Anemarrhena asphodeloides, rhubarb wine, Cortex Moutan, Rehmannia glutinosa, and dried ginger) in the type II diabetes therapy. Methods. TCMSP and Batman database (DB) were used to retrieve the chemical components and action targets of drugs; GeneCards, OMIM, TTD, DrugBank, and other databases were applied to screen the disease targets. We used the UniProt DB to annotate the targets before building the drug-compound-target network with Cytoscape 3.9.1. We also exploited the String DB to construct the protein-protein interaction (PPI) network. In addition, the targets for the treatment of type II diabetes were searched in the DrugBank, OMIM, GeneCards, and TTD database; then, we utilized Venn to intersect the key targets for the therapy of type II diabetes and active ingredient targets to obtain common targets. Furthermore, we exploited the common targets using GO and KEGG enrichment analysis method. The common targets and core components were analyzed by molecular docking using the AutoDock software. Results. A total of 61 effective components of this compound were screened out; drugs and type II diabetes have 278 common targets; the PPI network screened core target proteins such as CDKN1A, CDK2, and E2F1 with the help of molecular docking technology; the three main compounds including quercetin, kaempferol, and gamma-aminobutyric acid were obtained. Besides, the key target proteins had excellent binding properties with the main components. The signal pathways of six compound interventions in type II diabetes were mostly related to cancer, cocaine addiction, aminoacyl-tRNA biosynthesis, glycine, serine, threonine metabolism, platinum drug resistance, and other pathways, according to the KEGG enrichment analysis method. Conclusion. In the treatment of diabetes, the Huanglian Jiangtang formula has sorts of properties especially in the aspects of composition, target, and pathway. Its molecular target and mechanism of action may be related to pathways in cancer, cocaine addiction, aminoacyl-tRNA biosynthesis, glycine, serine, threonine metabolism, platinum drug resistance, and other pathways. This conclusion can provide theoretical support and science for further research.

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“…Inhibition of -amylase activity has been shown to be associated with some substances. These include tannins, phenols, flavonoids, and antioxidants (30) .…”

Section: Alph-amylase α-Amy Inhibitionmentioning

confidence: 99%

Inhibition, Characterization and Purification of α-AMY from Sera of Iraqi Breast Cancer Patients

Lafta¹,

Al-Qaisi²

2023

The Egyptian Journal of Hospital Medicine

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Background: α-Amylase enzyme is digestive enzyme that breaks down complex carbohydrates like starch into simpler sugars; it is being studied as a potential target for anti-cancer therapies. Objective: The current study aimed to evaluate the general characterization, and inhibition of α-Amylase enzyme, which had been purified from the sera of patients diagnosed with Breast Carcinoma. Materials and methods: A total of 100 blood serum samples were collected from patients with Breast cancer from Baghdad National Hope Hospital in Baghdad. α-Amylase enzyme was purified from serum clinical isolate in three stages; precipitation with 65% saturated ammonium sulphate, ion exchange chromatography utilizing a DEAEcellulose column, and gel filtration chromatography with a sephadex G-200 column. Changing conditions in pH, temperature, inhibitor concentration, and kinetics all affect the activity of the α-Amylase enzyme. Results: α-Amylase inhibition assay showed that Vitis vinifera L. was the most potent inhibitor (71.8%). During the kinetic investigation of the enzyme, it was discovered that the inhibitory mechanism that the extracts use is noncompetitive. Vmax was 0.023, and 0.012 mmol min -l at 75 μg/ml of Vitis vinifera L. , Verbena Officinalis L .extracts, respectively. Conclusions: High specific activity for α-Amylase purified from sera of Iraqi breast cancer patients was obtained after three purification steps. Ethanolic plant extracts showed inhibitory effect on α-Amylase while Vitis vinifera L. was the most potent inhibitor.

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Assessment of Antioxidative and Alpha-Amylase Potential of Polyherbal Extract

Cited by 13 publications

References 26 publications

Vancomycin as an Antibacterial Agent Capped with Silver Nanoparticles: An Experimental Potential Analysis

Vancomycin as an Antibacterial Agent Capped with Silver Nanoparticles: An Experimental Potential Analysis

To Investigate the Potential Mechanism of Huanglian Jiangtang Formula Lowering Blood Sugar in View of Network Pharmacology and Molecular Docking Technology

Inhibition, Characterization and Purification of α-AMY from Sera of Iraqi Breast Cancer Patients

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