Assessment of antioxidant system, cholinesterase activity and histopathology in Lithobates catesbeianus tadpoles exposed to water from an urban stream

Boiarski, Diego Romagna; Toigo, Camila Maria; Sobjak, Thaís Maylin; Santos, Andrey Felipe Potulski dos; Romão, Sílvia; Guimarães, Ana Tereza Bittencourt

doi:10.1007/s10646-020-02176-6

Cited by 6 publications

(3 citation statements)

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“…The slides were analyzed and photographed under a Leica DM 3000 photomicroscope attached to a Leica MC 170 HD camera. In the qualitative histopathological analyses, the parameters analyzed were the enlargement of the sinusoids, the vacuolization of the hepatocytes, the presence of hyperchromatic nuclei, pigmentation of the melanomacrophages, circulatory disturbances, steatosis, and inflammatory infiltration (Boiarski et al, 2020;Çakici, 2015).…”

Section: Histopathology and Ultrastructure Of The Livermentioning

confidence: 99%

“…This is why the liver is often the focus of environmental assessment studies (Boiarski et al, 2020). A number of studies have shown that the liver is the most informative type of tissue for the differentiation of animals in polluted and unpolluted environments associated with the use of pesticides on farmland in both urban and rural areas (Boiarski et al, 2020;Strong et al, 2016). Costa et al (2008) and Li et al (2017) showed the effects of herbicides and insecticides on the reactive oxygen species (ROS) of the anuran liver, although only a few studies have evaluated the morphological, histopathological, or hepatic metabolism of xenobiotic substances in anurans exposed to insecticides (Araújo et al, 2020;Çakici, 2015;Franco-Belussi et al, 2021), fungicides (Bernabo et al, 2017), and herbicides (Lopes et al, 2021;Oliveira et al, 2016;Pérez-Iglesias et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

“…The liver has a number of functions, including the metabolization and storage of the molecules absorbed during the digestion of foods, the secretion of plasmatic molecules, and the biotransformation of xenobiotic agents, and is involved in some of the transformative processes that occur during metamorphosis in anurans (Inoue et al, 2004). This is why the liver is often the focus of environmental assessment studies (Boiarski et al, 2020). A number of studies have shown that the liver is the most informative type of tissue for the differentiation of animals in polluted and unpolluted environments associated with the use of pesticides on farmland in both urban and rural areas (Boiarski et al, 2020; Strong et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

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The Use of Multiple Biomarkers to Assess the Health of Anuran Amphibians in the Brazilian Cerrado Savanna: An Ecotoxicological Approach

Lopes

Benvindo‐Souza

Sotero

et al. 2023

Enviro Toxic and Chemistry

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Changes in the natural landscape and the indiscriminate use of pesticides can have a major impact on aquatic environments and have contributed to the worldwide decline of amphibian populations. In the present study, we sampled tadpoles of three anuran amphibians (Boana albopunctata, Physalaemus cuvieri, and Dendropsophus minutus) from ponds in six different agricultural landscapes of the Brazilian Cerrado savanna and evaluated whether and to what extent genotoxic and mutagenic damage was related to land use (the amount of forest and agricultural remnants, and related physicochemical factors) and the presence of pesticides in the water of the study ponds. We also evaluated the hepatotoxicity in P. cuvieri, which was the most abundant species at five of the six sampling points. Clomazone and Atrazine were the most common pesticides found in the ponds. The B. albopunctata and P. cuvieri tadpoles presented similar patterns of DNA damage among the sampling points. The least DNA damage was found in the D. minutus tadpoles, although this species was present in only one of the study ponds. More binucleated and anucleated cells were observed in B. albopunctata, but there was no significant variation among species in terms of the number of micronuclei or other erythrocytic nuclear abnormalities. Land use and physicochemical factors did not explain the variation in the DNA damage observed in the three anurans. The hepatotoxicity analyses of P. cuvieri revealed the presence of a series of alterations, including the enlargement of the sinusoids, vacuolization of the hepatocytes, the infiltration of inflammatory cells, hepatic steatosis, and dilation of the blood vessels. The interaction between physicochemical factors and the biomarkers analyzed in this study is complex. In particular, it will be important to better elucidate which factors are contributing, either directly or indirectly, to the decline of anuran amphibian populations, especially in threatened biomes, such the the Brazilian Cerrado. In this case, we would encourage further in situ studies that assess the ecotoxicology of the landscape, together with the systematic monitoring of aquatic environments, in order to guarantee the long‐term integrity of amphibian populations, and those of other organisms that play an essential functional role in the ecosystem.

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Section: Histopathology and Ultrastructure Of The Livermentioning

confidence: 99%