Assessment of antioxidant and hepatoprotective potential of Satureja montana extracts against CCl4 induced liver damage

Abstract

The influence of Satureja montana extracts on several liver biochemical parameters in mice administered with carbon tetrachloride was estimated. In vivo investigation of antioxidant properties of S. montana extracts encompassed monitoring of biochemical parameters (derived from liver homogenate and blood hydrolyzate) and examination of potential hepatoprotective effect after intoxication with carbon tetrachloride. Biochemical tests included determination of activity of several antioxidant enzymes: xanthine oxidase, catalase, peroxidase, glutathione peroxidase, amount of reduced glutathione and intensity of lipid peroxidation. In order to obtain data about the potent hepatoprotective effect of examined extracts, the following parameters were determined: aspartate aminotransferase, alanine aminotransferase, bilirubin, hepatic DNA and hydroxyprolin. Results of this study demonstrate that S. montana extracts inhibited the hepatotoxicity produced by carbon tetrachloride administration most probably through activation of physiological common defense mechanisms.

Evaluation of Anticancer Activity of Satureja montana Supercritical and Spray-Dried Extracts on Ehrlich’s Ascites Carcinoma Bearing Mice

“…Additionally, Table 3 contains values of biochemical parameters of oxidative stress measured in the control group that was treated with distillated water and in groups that were treated only with SC1 and SD1 extracts [36]. By comparing these values with the parameters of oxidative stress determined in this study, it can be concluded that extracts exhibit selectivity towards tumor cells by inducing them into oxidative stress, while not manifesting any side-effects on the healthy cells.…”

“…Table 3. Biochemical parameters of control group and the groups treated with the SD1 and SC1 extracts [36].…”

“…The antioxidative activity of extracts was investigated by using the DPPH (2,2-diphenyl-1-picryl-hydrazyl-hydrate) assay. By comparing the manifested activities, expressed as the IC 50 value; that is, the concentration with which 50% of free radicals is inhibited, the dry extract showed a stronger antioxidative activity (SC 17.40 µg/mL; SD 5.24 µg/mL) [ 33 , 36 ]. The extract with the more dominant percentage of carvacrol did not exhibit a stronger antioxidative activity.…”

Enzymatic and Microwave Pretreatments and Supercritical CO2 Extraction for Improving Extraction Efficiency and Quality of Origanum vulgare L. spp. hirtum Extracts

“…Although after the pretreatment carvacrol content was increased by 25%, the total antioxidant response of the extracts was lowered [ 22 ]. Additionally, in in vivo studies where S. montana extracts with significantly different content of extracts were administered, it was observed that antioxidant properties of extracts with a higher content of carvacrol were not pronounced [ 72 , 73 ]. Therefore, carvacrol is not the main carrier of antioxidant activity of the extracts, but the properties of the extracts are a consequence of possible synergistic and antagonistic effects of all constituents.…”