Assessment of Antioxidant Activity and Neuroprotective Capacity on PC12 Cell Line of<i>Frankenia thymifolia</i>and Related Phenolic LC-MS/MS Identification

Mansour, Rim Ben; Megdiche-Ksouri, Wided; Cluzet, Stéphanie; Krisa, Stéphanie; Richard, Tristan; Ksouri, Riadh

doi:10.1155/2016/2843463

Cited by 12 publications

(11 citation statements)

References 46 publications

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“…In smaller concentrations, the protective effect against A β neurotoxicity tends to fade away, while in higher concentrations cytotoxic effects antagonize with the extracts' neuroprotective potential. Similar competition phenomena in relatively high concentrations have been observed in other plant species extracts, such as extracts derived from Frankenia thymifolia , Caliphruria subedentata , Piper sarmentosum , and Sasa senanensis [ 10 , 59 – 61 ].…”

Section: Discussionsupporting

confidence: 67%

Greek Sage Exhibits Neuroprotective Activity against Amyloid Beta-Induced Toxicity

Ververis

Savvidou

Ioannou

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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Alzheimer’s disease (AD) is the most common neurodegenerative disease, affecting the elderly at a high incidence. AD is of unknown etiology and currently, no cure is available. Present medication is restricted to treating symptoms; thus, a need exists for the development of effective remedies. Medicinal plants constitute a large pool, from which active compounds of great pharmaceutical potential can be derived. Various Salvia spp. are considered as neuroprotective, and here, the ability of Salvia fruticosa (SF) to protect against toxic effects induced in an AD cell model was partly assessed. Two of AD’s characteristic hallmarks are the presence of elevated oxidative stress levels and the cytotoxic aggregation of amyloid beta (Aβ) peptides. Thus, we obtained SF extracts in three different solvents of increasing polarity, consecutively, to evaluate (a) their antioxidant capacity with the employment of the free radical scavenging assay (DPPH•), of the ferric reducing ability of plasma assay (FRAP), and of the cellular reactive oxygen species assay (DCFDA) and (b) their neuroprotective properties against Aβ25–35-induced cell death with the use of an MTT assay. All three SF extracts showed a considerable antioxidant capacity, with the methanol (SFM) extract being the strongest. The results of the total phenolic and flavonoid contents (TPC and TFC) of the extracts and of the FRAP and the DCFDA assays showed a similar pattern. In addition, and most importantly, the dichloromethane (SFD) and the petroleum ether (SFP) extracts had an effect on Aβ toxicity, exhibiting a significant neuroprotective potential. To our knowledge, this is the first report of SF extracts demonstrating neuroprotective potential against Aβ toxicity. In combination with their antioxidant capacity, SF extracts may be beneficial in combating AD and other neurodegenerative diseases.

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Section: Discussionsupporting

confidence: 67%

Greek Sage Exhibits Neuroprotective Activity against Amyloid Beta-Induced Toxicity

Ververis

Savvidou

Ioannou

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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“…Radical scavenging activity against DPPH radical was measured as described by Mansour et al 15 , with minor modifications. A sample of 100 µL with various concentrations (10–4000 µg/mL) was mixed with 100 µL solution of DPPH in a 96-well plate.…”

Section: Methodsmentioning

confidence: 99%

Effect of particle size on phytochemical composition and antioxidant properties of Sargassum cristaefolium ethanol extract

Prasedya

Frediansyah

Martyasari

et al. 2021

Sci Rep

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Sample particle size is an important parameter in the solid–liquid extraction system of natural products for obtaining their bioactive compounds. This study evaluates the effect of sample particle size on the phytochemical composition and antioxidant activity of brown macroalgae Sargassum cristaefolium. The crude ethanol extract was extracted from dried powders of S.cristeafolium with various particle sizes (> 4000 µm, > 250 µm, > 125 µm, > 45 µm, and < 45 µm). The ethanolic extracts of S.cristaefolium were analysed for Total Phenolic Content (TPC), Total Flavonoid Content (TFC), phenolic compound concentration and antioxidant activities. The extract yield and phytochemical composition were more abundant in smaller particle sizes. Furthermore, the TPC (14.19 ± 2.08 mg GAE/g extract to 43.27 ± 2.56 mg GAE/g extract) and TFC (9.6 ± 1.8 mg QE/g extract to 70.27 ± 3.59 mg QE/g extract) values also significantly increased as particle sizes decreased. In addition, phenolic compounds epicatechin (EC), epicatechin gallate (ECG), epigallocatechin (EGC), and Epigallocatechin gallate (EGCG) concentration were frequently increased in samples of smaller particle sizes based on two-way ANOVA and Tukey’s multiple comparison analysis. These results correlate with the significantly stronger antioxidant activity in samples with smaller particle sizes. The smallest particle size (< 45 µm) demonstrated the strongest antioxidant activity based on DPPH, ABTS, hydroxyl assay and FRAP. In addition, ramp function graph evaluates the desired particle size for maximum phytochemical composition and antioxidant activity is 44 µm. In conclusion, current results show the importance of particle size reduction of macroalgae samples to increase the effectivity of its biological activity.

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“…It turns the solution from yellow to a blue complex of reduced molybdenum that can be detected spectrophotometrically at 750–765 nm. On the other hand, total flavonoid content (TFC), expressed as mg quercetin equivalent per gram of dry weight extract (mg QE/g DW), was assessed using an aluminum chloride-based colorimetric assay [ 23 ], and the scavenging ability of the extracts towards radical DPPH was also evaluated to establish their in vitro antioxidant activity [ 24 ]. Decolorization of the purple solution of DPPH to yellow indicates the reduction of DPPH following the interaction of radical DPPH with antioxidant compounds in the extract.…”

Section: Resultsmentioning

confidence: 99%

“…The antioxidant activity of C. timoriensis was measured using the DPPH method [ 24 ]. Briefly, a stock solution of each extract was prepared (1 mg/mL) using methanol.…”

Section: Methodsmentioning

confidence: 99%

Antioxidant, Anti-Inflammatory, and Inhibition of Acetylcholinesterase Potentials of Cassia timoriensis DC. Flowers

et al. 2021

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Despite being widely used traditionally as a general tonic, especially in South East Asia, scientific research on Cassia timoriensis, remains scarce. In this study, the aim was to evaluate the in vitro activities for acetylcholinesterase (AChE) inhibitory potential, radical scavenging ability, and the anti-inflammatory properties of different extracts of C. timoriensis flowers using Ellman’s assay, a DPPH assay, and an albumin denaturation assay, respectively. With the exception of the acetylcholinesterase activity, to the best of our knowledge, these activities were reported for the first time for C. timoriensis flowers. The phytochemical analysis confirmed the existence of tannins, flavonoids, saponins, terpenoids, and steroids in the C. timoriensis flower extracts. The ethyl acetate extract possessed the highest phenolic and flavonoid contents (527.43 ± 5.83 mg GAE/g DW and 851.83 ± 10.08 mg QE/g DW, respectively) as compared to the other extracts. In addition, the ethyl acetate and methanol extracts exhibited the highest antioxidant (IC50 20.12 ± 0.12 and 34.48 ± 0.07 µg/mL, respectively), anti-inflammatory (92.50 ± 1.38 and 92.22 ± 1.09, respectively), and anti-AChE (IC50 6.91 ± 0.38 and 6.40 ± 0.27 µg/mL, respectively) activities. These results suggest that ethyl acetate and methanol extracts may contain bioactive compounds that can control neurodegenerative disorders, including Alzheimer’s disease, through high antioxidant, anti-inflammatory, and anti-AChE activities.

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Assessment of Antioxidant Activity and Neuroprotective Capacity on PC12 Cell Line ofFrankenia thymifoliaand Related Phenolic LC-MS/MS Identification

Cited by 12 publications

References 46 publications

Greek Sage Exhibits Neuroprotective Activity against Amyloid Beta-Induced Toxicity

Greek Sage Exhibits Neuroprotective Activity against Amyloid Beta-Induced Toxicity

Effect of particle size on phytochemical composition and antioxidant properties of Sargassum cristaefolium ethanol extract

Antioxidant, Anti-Inflammatory, and Inhibition of Acetylcholinesterase Potentials of Cassia timoriensis DC. Flowers

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