Assessment of Antimicrobial Susceptibility of <i>Enterococcus Faecalis</i> Isolated From Chronic Periodontitis in Biofilm Versus Planktonic Phase

Sun, Jinglu; Song, Xianmin

doi:10.1902/jop.2010.100378

Cited by 23 publications

(21 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our previous study of oral Enterococcus faecalis isolated from Norwegian patients with marginal and apical periodontitis showed that approximately 50% of the strains were resistant to one or more of the tested antimicrobial agents, mostly tetracycline and/or erythromycin [5], [6]. These observations are consistent with former studies showing resistance towards commonly used antimicrobials in enterococci isolated from the oral cavity [7], [8], [9].…”

Section: Introductionsupporting

confidence: 88%

Characterisation of the Plasmidome within Enterococcus faecalis Isolated from Marginal Periodontitis Patients in Norway

et al. 2013

Self Cite

View full text Add to dashboard Cite

The present study aimed to identify and characterize plasmids in a national collection of oral Enterococcus faecalis (n = 106) isolated from patients with marginal periodontitis. Plasmid replicon typing was performed by multiplex-PCR and sequencing with specific primers for 18 rep-families and 1 unique sequence. Additional plasmid analysis by S1-PFGE was performed for comparison. Totally 120 plasmid replicon amplicons of seven rep-families were identified in 93 E. faecalis strains, e.g. rep9 (prototype pCF10), rep6 (prototype pS86), rep2 (prototype pRE25/pEF1), and rep8 (prototype pAM373). Rep9 was the most predominant rep-family being detected in 81 (76.4%) strains. Forty of these strains were tetracycline resistant and three were erythromycin resistant. Rep6 was the second predominant rep-family being detected in 22 (20.8%) strains. Rep2 was detected in eight (7.5%) strains. All rep2-positive strains were resistant to tetracycline and/or erythromycin and six of them contained Tn916/Tn1545 genes. The rep-positive E. faecalis exhibited divergence in multilocus sequence types (STs). There was a significant correlation between rep9 and ST21, while multiple rep-families appeared in ST40. Totally 145 plasmid bands were identified in 95 E. faecalis strains by S1-PFGE, 59 strains carrying one plasmid, 27 carrying two, five carrying three, three carrying four, and one strain carrying five plasmids. Plasmid sizes varied between 5–150 kbp. There was a significant correlation between the number of plasmids identified by PCR rep-typing and by S1-PFGE. The results indicate that the majority of E. faecalis of marginal periodontitis are likely to be a reservoir for diverse mobile genetic elements and associated antimicrobial resistance determinants.

show abstract

Section: Introductionsupporting

confidence: 88%

Characterisation of the Plasmidome within Enterococcus faecalis Isolated from Marginal Periodontitis Patients in Norway

et al. 2013

Self Cite

View full text Add to dashboard Cite

show abstract

“…11,20,23 However, it has been shown that biofilms formed in the first few days of incubation may not display the same antimicrobial resistance of a mature biofilm. 24,25 According to Guerreiro-Tanomaru et al, 20 the percentage of biofilm surface coverage on bovine dentine blocks was higher at 14 days than at 21 days, with no statistical differences between the two periods, thus justifying the 14 day-incubation period used in the present research.…”

Section: Discussionsupporting

confidence: 49%

Antimicrobial action of calcium hydroxide-based endodontic sealers after setting, against E. faecalis biofilm

et al. 2016

View full text Add to dashboard Cite

Antimicrobial action of calcium hydroxide-based endodontic sealers after setting, against E. faecalis biofilm Abstract: Enterococcus faecalis are gram positive bacteria that can mostly resist endodontic therapy, inducing persistent infection in the root canal system. Endodontic sealers with antimicrobial activity may help eliminate residual microorganisms that survive endodontic treatment. The present study aimed at comparing the antimicrobial activity of Acroseal, Sealapex and AH Plus endodontic sealers in an in vitro biofilm model. Bovine dentin specimens (144) were prepared, and twelve blocks for each sealer and each experimental time point (2, 7 and 14 days) were placed and left in contact with plates containing inoculum of E. faecalis (ATCC 51299), to induce biofilm formation. After 14 days, the samples were transferred to another plate with test sealers and kept at 37°C and 5% CO 2 for 2, 7 and 14 days. The specimens without sealers were used as a control for each period. The samples were agitated in a sonicator after each experiment. The suspensions were agitated in a vortex mixer, serially diluted in saline, and triple plated onto m-Enterococcus agar. Colonyforming units were counted, and the data were statistically analyzed using ANOVA, Shapiro-Wilk and Kruskal-Wallis one-way tests (p < 0.05) to determine antimicrobial potential. Sealapex showed significant differences at all the experimental time points, in comparison with all the other groups. AH Plus and Acroseal showed antimicrobial activity only on the 14 th experimental day. Neither of the sealers tested were able to completely eliminate the biofilm. Sealapex showed the highest antimicrobial activity in all the experimental periods. The antimicrobial activity of all the sealers analyzed increased over time.

show abstract

“…Enterococci are believed to be important biofilm participants (31–34). Even if enterococci are not present in high numbers in the dental plaque biofilm, they may have an important role as a reservoir for antibiotic-resistant genes, which can be transferred to other bacteria in the biofilm (12, 35). …”

Section: Discussionmentioning

confidence: 99%

Virulence factors and antibiotic susceptibility in enterococci isolated from oral mucosal and deep infections

Dahlén

Blomqvist

Almståhl

et al. 2012

Journal of Oral Microbiology

View full text Add to dashboard Cite

Objective This study evaluates the presence of virulence factors and antibiotic susceptibility among enterococcal isolates from oral mucosal and deep infections.Methods Forty-three enterococcal strains from oral mucosal lesions and 18 from deep infections were isolated from 830 samples that were sent during 2 years to Oral Microbiology, University of Gothenburg, for analysis. The 61 strains were identified by 16S rDNA, and characterized by the presence of the virulence genes efa A (endocarditis gene), gel E (gelatinase gene), ace (collagen binding antigen gene), asa (aggregation substance gene), cyl A (cytolysin activator gene) and esp (surface adhesin gene), tested for the production of bacteriocins and presence of plasmids. MIC determination was performed using the E-test method against the most commonly used antibiotics in dentistry, for example, penicillin V, amoxicillin and clindamycin. Vancomycin was included in order to detect vancomycin-resistant enterococci (VRE) strains.Results Sixty strains were identified as Enterococcus faecalis and one as Enterococcus faecium. All the virulence genes were detected in more than 93.3% (efa A and esp) of the E. faecalis strains, while the presence of phenotypic characteristics was much lower (gelatinase 10% and hemolysin 16.7%). Forty-six strains produced bacteriocins and one to six plasmids were detected in half of the isolates.Conclusions Enterococcal strains from oral infections had a high virulence capacity, showed bacteriocin production and had numerous plasmids. They were generally susceptible to ampicillins but were resistant to clindamycin, commonly used in dentistry, and no VRE-strain was found.

show abstract

Assessment of Antimicrobial Susceptibility of Enterococcus Faecalis Isolated From Chronic Periodontitis in Biofilm Versus Planktonic Phase

Cited by 23 publications

References 45 publications

Characterisation of the Plasmidome within Enterococcus faecalis Isolated from Marginal Periodontitis Patients in Norway

Characterisation of the Plasmidome within Enterococcus faecalis Isolated from Marginal Periodontitis Patients in Norway

Antimicrobial action of calcium hydroxide-based endodontic sealers after setting, against E. faecalis biofilm

Virulence factors and antibiotic susceptibility in enterococci isolated from oral mucosal and deep infections

Contact Info

Product

Resources

About