Assessment of antibiotic resistance of Escherichia coli isolates and screening of Salmonella spp. in wild ungulates from Portugal

Dias, Diana; Torres, Rita T.; Kronvall, Göran; Fonseca, Carlos; Mendo, Sónia; Caetano, Tânia

doi:10.1016/j.resmic.2015.03.006

Cited by 50 publications

(42 citation statements)

References 37 publications

(43 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The sampling strategy is known to have a major impact in the detection rate of salmonellae (Sanno et al., ; Wahlström et al., ). Besides the present work, where 2.9% of faecal samples from wild boars resulted positive for salmonellae, previous studies in the Iberian Peninsula reported highly variable prevalence values: 1.2% (Díaz‐Sánchez et al., ), 5% (Dias et al., ), 22.1% (Vieira‐Pinto et al., ) and 17.5%–35.6% (Navarro‐Gonzalez et al., ). Great variability has been found in other European regions as Italy, with values of 10.8% (Zottola et al., ) and 24.8% (Chiari, Zanoni, Tagliabue, Lavazza, & Alborali, ).…”

Section: Discussionsupporting

confidence: 50%

Prevalence ofSalmonellaspp. in tonsils, mandibular lymph nodes and faeces of wild boar from Spain and genetic relationship between isolates

Molino

Sánchez²,

Risco³

et al. 2019

Transbound Emerg Dis

View full text Add to dashboard Cite

Summary The importance of wild boars as game species in Spain is well known. Their feeding habits and intrusive behaviour, together with a progressively wider spreading of populations, increases the interactions of these animals with livestock and humans. Considering that wild boars could have a potential role in the transmission of certain pathogens as salmonellae, the aims of this study were to determine the prevalence of Salmonella spp. in wild boars hunted in central‐western Spain, the occurrence of this pathogen in tonsils, mandibular lymph nodes and faeces (as markers for transmission risk), and to define the phylogenetic relationships among isolated strains, in order to investigate the circulation pathways of bacteria among tissues, animals and estates. Samples from 1,041 hunted wild boars were analysed for the presence of Salmonella spp. by bacteriological culture. Isolates were confirmed by PCR and serotyped in the Spanish national reference laboratory. The genetic relationships between strains were determined by PFGE. The results showed a 7.7% of positive animals (81 wild boars), being tonsils the organ most frequently colonised by Salmonella spp. (18.7%), followed by lymph nodes (5.1%) and faecal samples (2.9%). Serovars Enteritidis and Newport were the most frequent amongst the 34 different serovars obtained. The pulsed‐field gel electrophoresis (PGFE) analysis showed a great genetic diversity, with serovars that exhibited different pulsotypes when isolated from different estates and multiple serovars in the same estate. In conclusion, this study reveals the importance of wild boars as carriers and possible transmitters of virulent and/or antimicrobial‐resistant clones of Salmonella spp. to livestock and humans.

show abstract

Section: Discussionsupporting

confidence: 50%

Prevalence ofSalmonellaspp. in tonsils, mandibular lymph nodes and faeces of wild boar from Spain and genetic relationship between isolates

Molino

Sánchez²,

Risco³

et al. 2019

Transbound Emerg Dis

View full text Add to dashboard Cite

show abstract

“…No resistance was detected against FOX and IPM. In Portugal, Dias et al 26 analyzing 152 E. coli from 67 fecal samples from wild ungulates, which represent the main prey for wolves, detected the highest resistance against AMC (16.45%); followed by AM (9.87%), TE (8.55%), S (4.61%), and FOX (0.66%). All isolates were susceptible to ATM, CTX, C, and IPM.…”

Section: Discussionmentioning

confidence: 99%

Antibiotic Susceptibility and Virulence Factors inEscherichia colifrom Sympatric Wildlife of the Apuan Alps Regional Park (Tuscany, Italy)

Turchi

Dec

Bertelloni

et al. 2019

Microbial Drug Resistance

View full text Add to dashboard Cite

Today a growing number of studies are focusing on antibiotic resistance in wildlife. This is due to the potential role of wild animals as reservoirs and spreaders of pathogenic and resistant bacteria. This study focused on isolating and identifying Escherichia coli from the feces of wild animals living in the Apuan Alps Regional Park (Tuscany, Italy) and evaluating some of their antibiotic resistance and pathogenicity traits. Eighty-five fecal samples from different species were studied. Seventy-one E. coli were identified by matrix assisted laser desorption ionization-time of flight mass spectrometry analysis, subjected to antibiograms and polymerase chain reaction for the detection of antibiotic resistance genes and pathogenicity factors. The highest resistance rates were found against cephalothin (39.4%) and ampicillin (33.8%), followed by amoxicillin/clavulanic acid (15.5%), streptomycin (12.7%), and tetracycline (5.6%). Regarding resistance genes, 39.4% of the isolates were negative for all tested genes. The remaining isolates were positive for bla CMY-2 , sul2, strA-strB and aadA1, tet(B), and tet(A), encoding resistance to beta-lactams, trimethoprim/sulfamethoxazole, streptomycin, and tetracycline, respectively. With regard to virulence factors, 63.4% of the isolates were negative for all genes; 21.1% carried astA alone, which is associated with different pathotypes, 9.9% carried both escV and eaeA (aEPEC); single isolates (1.4%) harbored escV (aEPEC), escV associated with astA and eaeA (aEPEC), astA with stx2 and hlyA (EHEC) or astA and stx1, stx2, and hlyA (EHEC). These results show that wildlife from nonanthropized environments can be a reservoir for antibiotic-resistant microorganisms and suggest the need for a deeper knowledge on their origin and diffusion mechanisms through different ecological niches.

show abstract

“…The application of antibiotics in various situations, from agriculture to the food industry, has resulted in a constant release of low-level antibiotic concentration into the water and soil through wastewater treatment plant effluents, sewage, agricultural waste and the application of manure fertilizer to fields and gardens, among others (Anon., 2006; Dias et al, 2015). This environmental pollution, together with co-habitation of livestock and wild animals and increased human contact, allows antibiotic compounds to reach more remote natural habitats.…”

Section: Antimicrobial Resistance In Wildlife Speciesmentioning

confidence: 99%

“…This environmental pollution, together with co-habitation of livestock and wild animals and increased human contact, allows antibiotic compounds to reach more remote natural habitats. This alters the population dynamics of microorganisms, encouraging the development of ABR in microbiological communities of wild animals through heightened selection pressure of resistance (Martínez, 2008;Dias et al, 2015). Importantly, water is a major transmission vector for antibiotic resistant microorganisms.…”

Section: Antimicrobial Resistance In Wildlife Speciesmentioning

confidence: 99%

Importance and implications of antibiotic resistance development in livestock and wildlife farming in South Africa: A Review

Honert¹,

Gouws²,

Hoffman³

2018

SA J. An. Sci.

View full text Add to dashboard Cite

Antibiotic resistance (ABR) is regarded as one of today's major global health challenges. The development of ABR in nature is a complex phenomenon with many influencing factors, of which the farming industry is labelled a significant contributor. The transfer of ABR to humans, which usually occurs via the food chain, is of concern for human health. A food source that is increasing in popularity is game meat, which is farmed widely in South Africa. The natural environment, including wildlife, is not isolated from the rest of the farm, and thus could be a source of ABR or possibly a transfer vector. It is therefore important to assess the ABR situation in wildlife species and the factors that influence its emergence and transfer. Elements that play a part in the development of ABR in game species include certain harvesting and slaughtering and other farming practices and closer contact with humans and other farm animals. Additionally, natural transfer vectors include wind, water, manure, crops and animals. Worryingly, there is lack of knowledge of this situation owing to inadequate monitoring, documentation and control of antibiotics in the farming industry. The objective of this review was to gain better understanding of this situation, which would aid in the development of surveillance systems and methods to prevent or hinder the development of ABR in wildlife species. ______________________________________________________________________________________

show abstract

Assessment of antibiotic resistance of Escherichia coli isolates and screening of Salmonella spp. in wild ungulates from Portugal

Cited by 50 publications

References 37 publications

Prevalence ofSalmonellaspp. in tonsils, mandibular lymph nodes and faeces of wild boar from Spain and genetic relationship between isolates

Prevalence ofSalmonellaspp. in tonsils, mandibular lymph nodes and faeces of wild boar from Spain and genetic relationship between isolates

Antibiotic Susceptibility and Virulence Factors inEscherichia colifrom Sympatric Wildlife of the Apuan Alps Regional Park (Tuscany, Italy)

Importance and implications of antibiotic resistance development in livestock and wildlife farming in South Africa: A Review

Contact Info

Product

Resources

About