Assessment of Antibacterial and Antifungal Properties and In Vivo Cytotoxicity of Peruvian Passiflora mollisima

Abstract

Aim:The antimicrobial activity of Passiflora mollissima is largely due to its aliphatic and aromatic hydrocarbons, terpenes, alkaloids, ketones, esters, alcohols, and lactones. The objective of this research was to evaluate the antimicrobial activity of the compounds in the Passiflora mollissima (Tumbo) fruit and leaves included also on cultured strains of the microorganisms Streptococcus mutans, Streptococcus oralis, Streptococcus sanguinis and Candida albicans.Materials and methods: Antimicrobial activity was analyzed by the disk diffusion method and evaluated in terms of their zones of inhibition. The cytotoxic activity of these compounds was determined in a bioassay involving sea urchin eggs (Tetrapygusniger) in which the inhibition percentage of artificially fertilized ovules was equivalent to their cytotoxic activity after 26 hours. Results:This study demonstrated the antimicrobial activity of ethanolicPassiflora mollissima extract against the cultured strains of Streptococcus mutans, Streptococcus oralis, and Streptococcus sanguinis with zones of inhibition after the incubation period. Conclusion:There was no evidence of activity against the pathogenic Candida albicans, which demonstrates a null antifungal capacity of the evaluated extracts. However, cytotoxic activity was found, and the compounds led to the complete inhibition of 100% of sea urchin ovules after 26 hours of exposure. Clinical importance:The results of the study serve as a guide. in the industrial use of this natural resource "tumbo" due to the discovery of a potential medicine based on this plant that would be a great development in the field of antimicrobial therapies.

Antibacterial Effectiveness of Four Concentrations of the Hydroalcoholic Extract of Solanum tuberosum (Tocosh) against Streptococcus mutans ATCC 25175TM: A Comparative In Vitro Study

“…Multiple studies have demonstrated its important medicinal properties. However, its antibacterial effectiveness against S. mutans , which has shown a relevant role in the pathogenesis of different oral diseases, has not yet been demonstrated [ 3 – 5 ]. Tooth decay is one of the most prevalent diseases in Peru, and it is associated with different etiological factors.…”

Antifungal efficacy of four different concentrations of the essential oil of Cinnamomum zeylanicum (Canela) against Candida albicans: An in vitro study

“…Direct observation was made, and the measurement of the inhibitory halo (Kirby–Bauer method)[ 6 8 9 10 ] of four concentrations at 25%, 50%, 75%, and 100% of the EOC and nystatin on strains of Candida albicans ATCC 10231 was taken; the data obtained was recorded in a data collection sheet where the inhibition halos measurements in millimeters, formed in each of the discs embedded with the EOC. In the period of 24 and 48h of having made the sowing, the readings and filling of the collection sheet were made, taking into account the identification of the sample, identification of the concentration used, and the measurement in millimeters of the halo of inhibition formed [ Figure 2 ].…”

“…It also refers to the intervention to recover health through the use of herbs with medicinal properties or their derivatives. [ 8 9 ]…”

Antibacterial efficacy of calcium hydroxide with iodoform versus calcium hydroxide with camphorated paramonochlorophenol as intrachannel pastes on an Enterococcus faecalis biofilm: A comparative in vitro study

“…Several studies have evaluated the use of natural products as a new antimicrobial alternative,[ 8 9 10 11 ] and have described the importance of the application of substances such as intraconduction medication in pieces with asymptomatic apical periodontitis to eliminate existing bacteria, and also to achieve successful duct treatment.…”