Assessment of anti-inflammatory potential of Sesbania bispinosa Linn. leaf extracts and fractions by acute and chronic models

Abstract

Aim and objectives: Leaf extracts and fractions of S. bispinosa were evaluated for anti-inflammatory activity in mice using acute and chronic anti-inflammatory models with aspirin as a reference drug. Materials and methods: Methanol, chloroform and hexane were used to prepare leaf extracts by soxhlet extraction method, while acetone, ethyl acetate and petroleum ether were used to prepare fractions of most active extract. These extract and fractions were evaluated by using carrageenan and formalin-induced inflammation models in albino mice. Extracts and fractions at the dose 250 mg/kg/p.o. and aspirin at 100 mg/kg/p.o. were used for the study. Results: Methanolic extract of S. bispinosa (MELSB) revealed significant anti-inflammatory activity which is indicated by 15.22% inhibition of paw edema at 180 min in carrageenan model, while ethyl acetate fraction of methanolic extract showed 16.26% and 15.50% inhibition of paw edema and thickness in carrageenan and formalin models respectively. In chronic model, just like aspirin, time-dependent activity was observed with acetone and ethyl acetate fractions; the greater reduction in paw thickness was observed with ethyl acetate fraction ( ** p < 0.01). Conclusion:The results of the present study suggest that leaves of S. bispinosa possess significant level of anti-inflammatory activity and ethyl acetate fraction may be further explored as an anti-inflammatory remedy as it was found to possess higher anti-inflammatory activity among all extracts and fractions as demonstrated in both acute and chronic models.

Scenario of <i>in Vitro </i>Regeneration and Transformation Studies in Multipurpose <i>Sesbania </i>Species

“…These are in cultivation mainly for soil improvement as bio-fertilizers and agroforestry as well as also grown to provide us food, medicines, wood, fibre, dyes and fuels [1]. Different Sesbania species had reported to possess different pharmacological properties like antibacterial properties in S. aculeata [2], anti-inflammatory properties in S. bispinosa [3], anticancer and antidiabetic activity in S. grandiflora [4,5]. In India S. rostrata is the most widely used Sesbania species that possess various medicinal properties and is used to treat various ailments such as rheumatism, gout, leprosy, inflammation, bronchitis, anaemia, headaches, fevers, anxiolytic [6].…”

Extraction and Characterization of <i>Sesbania cannabina</i> (Retz.) Pers. (Dhaincha) Seed Oil for Potential Engineering Applications

“…The leaves, flowers, pods and seeds of Sesbania constitute a valuable source of livestock feed. Sesbania leaves contain 30％ protein and good amino acid composition and are therefore considered a high quality fodder for livestock with reported increase in milk and meat production 12) . Sesbania seeds contain 29-33％ crude protein, 4.7-6％ crude lipid, 11-16％ crude fibre, and 44-47％ carbohydrates 13) .…”

“…The roots of Sesbania are used as alexiteric (against the effects of poison) , anthelmintic (to destroy parasitic worms) , carminative (to prevent formation of gas in the gastrointestinal tract and facilitate its expulsion) , collyrium (medicated eyewash) , contraceptive (to prevent pregnancy) , diuretic (causing increased passing of urine) , galactagogue (to promote the flow of mother s milk) and hepatoprotective (to protect damage to the liver) . Some other pharmaceutical applications of roots include the treatment of dysuria (painful urination) , leucoderma (loss of skin colour) , fever, ulcer, and diabetes 12,16,17) . Similarly, barks are effective in treatment of anaemia, bronchitis, dysentery, inflammations, leucorrhoea, liver cirrhosis, tumour and hypertension 12) .…”

“…Some other pharmaceutical applications of roots include the treatment of dysuria (painful urination) , leucoderma (loss of skin colour) , fever, ulcer, and diabetes 12,16,17) . Similarly, barks are effective in treatment of anaemia, bronchitis, dysentery, inflammations, leucorrhoea, liver cirrhosis, tumour and hypertension 12) .…”

“…The extract of Sesbania leaves in the form of tea is believed to contain anthelmintic, antibiotic, antispasmodic, antitumor, contraceptive 14,18) and sedative properties. Leaf paste is often used in seeking relief from skin diseases and inflammatory rheumatism 12) . Leaves wash is used as prevention to tsetse flies and is also given to infants against a whooping cough 17) .…”