Assessment of anti-diabetic activity of an ethnopharmacological plant Nerium oleander through alloxan induced diabetes in mice

Dey, Priyankar; Saha, Manideepa; Chowdhuri, Sumedha Roy; Sen, Arnab; Sarkar, Mousumi Poddar; Haldar, Biswajit; Chaudhuri, Tapas Kumar

doi:10.1016/j.jep.2014.12.012

Cited by 47 publications

(23 citation statements)

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“…Furthermore, the anti-hyperlipidemic activities were evident through lowering of cholesterol levels and associated lowering of fatty infiltrations and steatosis under the histopathological studies, which were further supported by the previous studied of Gayathri et al [30]. The blood glucose level lowering capacity of the oleander extracts is also supported by its ethnopharmacological claim of hypoglycaemic activities as well as a recent study that demonstrated anti-diabetic activities of oleander leaf under alloxan induced diabetes in mouse [35]. CCl 4 toxicity in the present study, resulted in significant ( p < 0.001) elevation of ACP, ALP, AST, ALT, GGT, LDH, glucose, urea, globulin, bilirubin and cholesterol levels, signifying liver injury.…”

Section: Discussionsupporting

confidence: 71%

Haloalkane induced hepatic insult in murine model: amelioration by Oleander through antioxidant and anti-inflammatory activities, an in vitro and in vivo study

Dey

Dutta

Biswas-Raha

et al. 2016

BMC Complement Altern Med

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BackgroundNerium oleander L. (syn. Nerium indicum Mill, Nerium odorum Aiton) belongs to the family Apocynaceae. It is used for its anti-inflammatory, anti-diabetic, anti-cancer and hepatoprotective activities in traditional medicine. Previous pharmacognostic studies suggested that 70 % hydro-methanolic extracts of oleander possess potent free radical scavenging and anti-inflammatory activities, both of which are helpful against hepatotoxicity.MethodsHydro-methanolic extracts of oleander stem and root were evaluated for their hepatoprotective activities in acute CCl4 intoxicated mouse through in vitro and in vivo studies. Silymarin was used as positive reference. Antioxidant enzymes, pro-inflammatory markers and liver enzymatic and biochemical parameters were studied. The extracts were further chemically characterized using Fourier Transform Infrared (FTIR) spectroscopy and Gas chromatography-mass spectrometry (GC-MS).ResultsCCl4 toxicity caused fatty liver formation by increase of relative liver weight (32.53 g) compared to control group (16.08 g). The elevated liver enzymatic and biochemical parameters due to CCl4 toxicity were considerably normalized by the extracts treatment under both in vivo and in vitro models. Oleander stem (NOSE) and root (NORE) extracts increased the reduced hepatic catalase activity 27.37 and 25.25 %, whereas peroxidase activity was increased 18.19 and 22.78 %, respectively. The extent of lipid peroxidation was significantly (p < 0.01) lowered 20.76 % (NOSE) and 21.12 % (NORE) compared to CCl4 group. The levels of pro-inflammatory tumor necrosis factor-α (TNF-α) was lowered 71.33 % (NOSE) and 61.60 % (NORE). Histopathological study demonstrated substantial reduction of hepatocellular necrosis, fatty infiltration, sinusoidal dilation, bile duct proliferation, vascular congestion, leukocyte infiltration in the silymarin and extract treated groups. Furthermore, various bioactive compounds were identified in the extracts such as apocynin, tocopherol, squalene, vanillin, isoeugenol, amyrin, lupeol etc.ConclusionThe present study provided convincing evidence that oleander extracts possess potent hepatoprotective capacity which was primarily governed by its antioxidant and anti-inflammatory activities. The collegial bioactivities of the phytochemicals may be accredited behind the hepatoprotective activity of oleander.Electronic supplementary materialThe online version of this article (doi:10.1186/s12906-016-1260-4) contains supplementary material, which is available to authorized users.

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Section: Discussionsupporting

confidence: 71%

Haloalkane induced hepatic insult in murine model: amelioration by Oleander through antioxidant and anti-inflammatory activities, an in vitro and in vivo study

Dey

Dutta

Biswas-Raha

et al. 2016

BMC Complement Altern Med

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“…Blood was withdrawn by orbital cardiac puncture and immediately assayed for reduced glutathione (GSH) and oxidized glutathione (GSSG) levels using a commercial glutathione assay kit (Caymann Chemical, Bertin Pharma, France). Samples of blood hemolyzates were used for hemoglobin (Hb) and glycosylated hemoglobin (HbA 1C , expressed as percentage of total Hb content) determination as described (Dey et al, 2015). Plasma samples were isolated by centrifugation (1650g for 15 min at 3 °C) and stored at 80 °C for further analysis of glucose and insulin levels, and for assaying protein carbonyls and inflammatory markers (see below).…”

Section: Oral Glucose Tolerance Test (Ogtt) In Long Term-treated Micementioning

confidence: 99%

Antidiabetic, antioxidant and anti inflammatory properties of water and n-butanol soluble extracts from Saharian Anvillea radiata in high-fat-diet fed mice

Kandouli

Cassien

Mercier

et al. 2017

Journal of Ethnopharmacology

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“…Diabetes mellitus (DM) is a chronic disease, and type 2 DM is characterized by disorder in carbohydrate, lipid and fat metabolism which severely affects the various organs of the body 1 . DM would cause various complications such as renal failure, cardiovascular disease, blindness or other liver disease 2 .…”

Section: Introductionmentioning

confidence: 99%

Gc-MS Profiling, Chemical Characterization, Antioxidant, Α-Amylase and Α-Glucosidase Inhibition of Selected Seaweeds From Southeast Coast of India: An in Vitro Approach

Deepak

Sowmiya²,

Kamaraj

et al. 2018

J. Drug Delivery Ther.

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The present study focuses on in vitro antioxidant and enzyme inhibitory activity of three different solvent extracts (Methanol, Ethyl acetate and Hexane) of 3 different seaweeds viz: Sargassum wightii, Caulerpa racemosa, and Acanthophora spicifera. The preliminary phytochemical analyses of the seaweed extracts have revealed the presence of various phytochemicals. The antioxidant activities of the seaweed extracts have shown the scavenging effect. Among the extracts, SWEA, SWME and SWHE have exerted effective antioxidant activity with the IC 50 values (µg/mL) of: 32.86, 130.1 and 212.8, respectively. And similar trend of α-amylase and α-glucosidase activity/inhibitory property by seaweeds have been recorded. Hence, the ethyl acetate extract of S. wightii was subjected to gas chromatography. All the seaweed extracts were characterized through HPLC and FTIR analyses. The GC-MS analysis of ethyl acetate extract of S. wightii showed the presence of a bioactive compound, Heptadecanoic acid that might be have been the reason for the recorded inhibitory activity.

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Assessment of anti-diabetic activity of an ethnopharmacological plant Nerium oleander through alloxan induced diabetes in mice

Cited by 47 publications

References 40 publications

Haloalkane induced hepatic insult in murine model: amelioration by Oleander through antioxidant and anti-inflammatory activities, an in vitro and in vivo study

Haloalkane induced hepatic insult in murine model: amelioration by Oleander through antioxidant and anti-inflammatory activities, an in vitro and in vivo study

Antidiabetic, antioxidant and anti inflammatory properties of water and n-butanol soluble extracts from Saharian Anvillea radiata in high-fat-diet fed mice

Gc-MS Profiling, Chemical Characterization, Antioxidant, Α-Amylase and Α-Glucosidase Inhibition of Selected Seaweeds From Southeast Coast of India: An in Vitro Approach

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