Abstract:The androgenic potential of a New Zealand pulp mill effluent (PME) and a Canadian PME was assessed along with a New Zealand sewage treatment plant effluent (STP) using a combination of in vivo and in vitro methods. The in vitro methods included: (1) a fish-based androgen receptor binding assay, (2) a fish-based aromatase inhibition activity assay, and (3) an analysis of gonadal sex steroid levels in exposed female mosquitofish ( Gambusia affinis ) ovaries by radioimmunoassay. The in vivo method included a quan… Show more
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