Assessment of an open-access CASA software for bovine and buffalo sperm motility analysis

Prete, Chiara Del; Blanco-Prieto, Olga; Mislei, Beatrice; Iacono, Eleonora; Mari, Gaetano; Cocchia, N; Gasparrini, B.; Merlo, Barbara; Bucci, Diego

doi:10.1016/j.anireprosci.2022.107089

Cited by 1 publication

(2 citation statements)

References 40 publications

(56 reference statements)

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“…Flow cytometric data were analysed using FlowJo procedures (FlowJo, LLC, Ashland, OR, USA). Del Prete et al (2022) use the FACSCalibur flow cytometer (Becton Dickinson, Milan, Italy) equipped with a 488 nm argon-ion laser and a 635 nm red diode laser was used in this study with the following filters: 530/30 band-pass (green/ FL-1), 585/42 band-pass (orange/FL-2), > 670 long https://doi.org/10.17221/152/2023-CJAS for assessing plasma membrane damage (Savvulidi et al 2021). Subsequently, sperm samples were analysed using a NovoCyte 3000 digital flow cytometer (Acea Biosciences, part of Agilent, Santa Clara, CA, USA).…”

Section: Evaluation Of Sperm Plasma Membrane Integritymentioning

confidence: 99%

“…Several methodologies of JC-1 usage were described in recent publications. For instance, flow cytometry analysis with JC-1 was performed to evaluate mitochondrial membrane potential (Ψm) in the study of Del Prete et al (2022). In this study, the FACSCalibur flow cytometer (Becton Dickinson, Milan, Italy) was equipped with a 488 nm argon-ion laser and a 635 nm red diode laser was used with the following filters: 530/30 band-pass (green/FL-1), 585/42 band-pass (orange/FL-2), > 670 long pass (far-red/FL-3) and 661/16 band-pass (orange far red laser/FL-4).…”

Section: Evaluation Of Mitochondrial Statusmentioning

confidence: 99%

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Meta-analysis of fluorochrome concentrations to assess bull sperm quality by flow cytometry in 2021-2023

Savvulidi,

Ptáček,

Nagy

et al. 2023

CZECH J ANIM SCI

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Flow cytometry (FC) is a very powerful method for the assessment of spermatozoa quality in multiple livestock species, including bulls. Nowadays, many flow cytometric tests are available for quality detection of bull sperm. These tests are based on fluorochromes (fluorescent tags or dyes) and the use of correct concentrations of fluorochromes is an essential aspect of the successful optimization of experimental methodology. Modern flow cytometers are much more affordable, such that spermatologists are now able to use FC methodology (and even image cytometry) in their studies. The present review aims to give a summary of current flow cytometric methodologies used to assess bull sperm quality by flow cytometry. Namely, we give here the concentrations of fluorochromes used in the studies related to bull sperm quality evaluation by flow cytometry published in the last three years (from 2021 to 2023). Importantly, in the present review, the concentration of fluorochromes is unified and presented using comparable molarity units. Furthermore, the rationale of each flow cytometric methodology for particular fluorescent tests, and the citation for the corresponding original methodology, are given. This review will help spermatologists shorten the time needed for the optimization of their flow cytometry methodology for the assessment of bull sperm quality.

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Section: Evaluation Of Sperm Plasma Membrane Integritymentioning

confidence: 99%

Section: Evaluation Of Mitochondrial Statusmentioning

confidence: 99%