Assessment of <i>Beauveria bassiana</i> and Their Enzymatic Extracts against <i>Metamasius spinolae</i> and <i>Cyclocephala lunulata</i> in Laboratory

Abstract

The application of enzymatic extracts and conidia of Beauveria bassiana in Metamasius spinolae and Cyclocephala lunulata was evaluated. The variables were mortality and time of death. In M. spinolae, mortality with extracts 29%, conidia 27% and the combination of both 31%, all had a time of death of four days. Although with different symptoms, used enzymatic extracts: contraction and softening of the joints; by conidia: mycelium in the joints; in the combination of conidia and enzymatic extracts: abundant aerial mycelium. In C. lunulata, 100% mortality in all treatments; Time of death: enzymatic extracts and extracts with conidia 1.2 days; conidia 2.8 days. Symptoms were different, enzymatic extracts: melanization and internal tissue lysis; enzymatic extract and conidia: mycelium emerged and melanization; conidia: mycelium emerged. Enzymatic extracts showed insecticidal activity in M. spinolae and C. lunulata. These results suggest the potential of enzymatic extracts as biocontrol agents to improve the use of entomopathogenic fungi.

Effectiveness of Secondary Metabolites from Entomopathogenic Fungi for Control Nilaparvata lugens Stål. in the Laboratory Scale

“…The biological activity of each secondary metabolite produced by each fungus can also be seen in .00 c 100.00 m 100.00 k 100.00 l 100.00 m 100.00 j 100.00 k 1.14 a Table 1. Mortality (percent) and time of death (days) of brown planthopper at 1 to 7 days after application of secondary metabolites of entomopathogenic fungi The toxin production will differ depending on fungal isolates, culture composition, and pH so that the culture extracts or filtrates from different fungi are thought to contain secondary metabolites or compounds that have different insecticide activity (Sánchez-Pérez et al, 2016). The type and concentration of a compound can vary according to fungal isolates, the composition of the culture medium, and the conditions of propagation (Valencia et al, 2011;Safavi, 2013).…”

Production of Sustainable Proteins Through the Conversion of Insects to Proteins Using Beauveria bassiana Cultures

“…In this way, it would be possible to produce high quality sustainable protein that is widely accepted by consumers. Entomopathogenic fungi exude chitinolytic enzymes, in addition to proteins, to penetrate the outer cuticle of insects (Mondal et al, 2016;Sánchez-Pérez et al, 2016). These chitinases degrade chitin polymers to N-acetylglucosamine units and are used in the food industry for the fermentation of shellfish, crab, and shrimp shells (Le and Yang, 2019).…”