Assessment of allyl isothiocyanate as a fumigant to avoid mycotoxin production during corn storage

Evaluation of gaseous allyl isothiocyanate against the growth of mycotoxigenic fungi and mycotoxin production in corn stored for 6 months

“…The experiment was conducted in triplicate and the analyses were carried out in duplicate ( n = 6). The extraction was performed as described by Tracz et al . Samples (5 g) were placed in a 15 mL Falcon tube containing 10 mL methanol.…”

“…FB 2 was not detected in the samples throughout the study (data not shown). Tracz et al . evaluated the effect of gaseous AITC on fumonisin production in corn kernels treated with 50, 100, and 500 µL L −1 during 30 days.…”

“…The experiment was conducted in triplicate and the analyses were carried out in duplicate (n = 6). The extraction was performed as described by Tracz et al 32 Samples (5 g) were placed in a 15 mL Falcon tube containing 10 mL methanol. The mixture was mixed for 30 min in water bath at 40 ∘ C. Thereafter, tubes were placed inside an ultrasonic bath for 10 min, followed by centrifugation at 4000 × g for 5 min at 20 ∘ C. The supernatant was recovered and filtered through 0.22 μm nylon membrane.…”

“…Allyl isothiocyanate (AITC), derived from brown and black mustard, is a volatile compound that has presented potent antimicrobial activity. Its volatility and small molecular weight enables its penetration in stored foods through fumigation, avoiding mold growth and mycotoxin production in different foods, including bread, pizza crust, nuts, wheat tortillas, wheat flour, strawberries, and corn kernels …”

“…Its volatility and small molecular weight enables its penetration in stored foods through fumigation, 24,25 avoiding mold growth and mycotoxin production in different foods, including bread, 26 pizza crust, 27 nuts, 28 wheat tortillas, 29 wheat flour, 30 strawberries, 31 and corn kernels. 32 The objective of this study was to evaluate the effects of AITC as fumigant to inhibit the growth of A. parasiticus CECT 2947 and F. verticillioides CECT 2983 and reduce mycotoxin production in corn kernels during 180 days of storage. Moreover, the residual concentrations of AITC in the corn matrix was evaluated during the same period to verify when reapplications of the fumigant would be necessary.…”