Assessment of agronomic, chemical and genetic variability in common basil (Ocimum basilicum L.)

Masi, Luigi De; Siviero, P.; Esposito, Castrese; Castaldo, Domenico; Siano, Francesco; Laratta, Bruna

doi:10.1007/s00217-005-0201-0

Cited by 92 publications

(55 citation statements)

References 22 publications

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“…Basil leaves are largely employed as a flavoring agent for food. For instance, sweet basil is the main ingredient of the well-known 'pesto' sauce and in Italy the cultivation of this species increased considerably in the last years due to the growing demand from the food industry [2]. Along with other species in the Ocimum genus, sweet basil is used for pharmaceutical and cosmetic preparations due to the high content of essential oils [1,3] and rosmarinic acid [4,5].…”

Section: Introductionmentioning

confidence: 99%

Rosmarinic acid content in basil plants grown in vitro and in hydroponics

et al. 2011

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The accumulation of selected caffeic acid derivatives (CADs), in particular rosmarinic acid (RA), was investigated in different tissues (leaves, roots and plantlet shoots) of sweet basil (Ocimum basilicum L.) plants grown either in vitro or in hydroponic culture (floating system) under greenhouse conditions. Two cultivars with green leaves (Genovese and Superbo) and one with purple leaves (Dark Opal) were tested. The content of CADs in HCl-methanol extracts was determined by HPLC. LC-MS and LC-MS-MS were used to confirm the identification of the metabolites of interest. Apart from rosmarinic acid (RA) and a methylated form of this substance, no other CADs were detected at significant level in any of the analyzed samples. The content of RA ranged approximately from 4 to 63 mg/g DW, depending on the growing system. The highest RA content was found during the in vitro multiplication, in the acclimatized plants and in the roots of hydroponically-grown seedlings at full bloom. In vitro, 6-benzyladenine reduced the accumulation of RA in purple-leaf Dark Opal cultivar, but an opposite effect of this growth regulator was observed in the green-leaf genotypes. Our findings suggest the possibility to scale-up RA production by means of in vitro or hydroponic culture of sweet basil.

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Section: Introductionmentioning

confidence: 99%

Rosmarinic acid content in basil plants grown in vitro and in hydroponics

et al. 2011

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“…O. basilicum has been cultivated from ancient times both as an ornamental plant and as a major essential oil crop. Although essential oils in different basil cultivars are variable, the prevalent chemical components are phenylpropanoids, such as estragole, eugenol, methyl-eugenol and methyl-cinnamate, and monoterpenes, such as linalool, geranial, neral and eucalyptol (De Masi et al, 2006). Scientific studies have also shown that bioactive constituents in basil oil are antioxidant, anticancer, antimicrobial, antiviral, antifungal, repellent, insecticidal, or nematicidal (Tsai et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

Pollination following grafting introduces efficiently Ocimum basilicum L. genes into Nicotiana tabacum L.

Wei

Yang

Wei

2013

Span. j. agric. res.

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Tobacco is an important cash crop in the world. However, the genetic basis is comparatively narrow among the modern Nicotiana tabacum cultivars, limiting its potential for quality improvement. To introduce genes conferring desirable chemical constituents from medicinal plants, a distant hybridization test was conducted between N. tabacum and Ocimum basilicum L. Seedlings of wild type Nicotiana sylvestris and N. tabacum cultivar 78-04 respectively acted as rootstock and scion. During the flowering season, hand pollination between 78-04 as pistillate parent and O. basilicum as pollen parent was carried out under 22-25°C temperature and 70-80% of relative humidity in the greenhouse. Seed sets of 55% were obtained in 78-04, and about 400 seeds per capsule were produced. But both non-grafted and self-grafted 78-04 plants rarely resulted in fruits by hand pollination and those obtained were without seed. Similar results were obtained in different material combination. The interfamilial F1 hybrids acquired showed distinct variation with various morphological characteristics, and their hybrid nature was confirmed by isozyme and random amplified polymorphic DNA (RAPD) analyses. This result indicated that pollination following grafting can facilitate gene exchange and recombination at the interfamilial level and efficiently overcome barriers of sexual incompatibility between N. tabacum and O. basilicum. Our research not only extends the genetic basis of tobacco but also will provide valuable germplasm for improvement of varieties.

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“…Data Analysis. Chemotype classification was based on a system that considers the major chemical components rather than a single dominant compound [13]. Each chemotype was defined by the compounds that were found in concentrations higher than 10%.…”

Section: Experimental Partmentioning

confidence: 99%

Chemical Characterization and Genetic Relationships among Ocimum basilicum L. Cultivars

Liber

Carović‐Stanko

Politeo

et al. 2011

Chemistry & Biodiversity

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Twenty-seven Ocimum basilicum cultivars were subjected to a chemical characterization of essential oil components by gas chromatography/mass spectrometry (GC/MS) and a genetic characterization using the amplified fragment-length polymorphism (AFLP) technique. Since the same 27 accessions had previously been classified into six morphotypes, these analyses allowed us to make detailed comparisons of chemistry, genetics, and morphology. The chemical composition and morphology of the studied cultivars appeared to have a strong genetic component. The AFLP analysis revealed a distinction between the green and purple morphotypes. The green morphotypes predominantly utilized the terpene biosynthetic pathway, while most purple morphotypes primarily utilized the phenylpropene biosynthetic pathway. The GC/MS analysis led to identification of 87 volatiles. Among the 27 cultivars, five chemotypes were identified. A detailed characterization of the essential oil constituents indicated the existence of both specific combinations of compounds and 'private' compounds with the potential to be used in many aspects of human life. The established relationship between a genetic profile, chemical composition, and morphology represents an important step in future breeding programs and in the cultivation of this species.

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Assessment of agronomic, chemical and genetic variability in common basil (Ocimum basilicum L.)

Cited by 92 publications

References 22 publications

Rosmarinic acid content in basil plants grown in vitro and in hydroponics

Rosmarinic acid content in basil plants grown in vitro and in hydroponics

Pollination following grafting introduces efficiently Ocimum basilicum L. genes into Nicotiana tabacum L.

Chemical Characterization and Genetic Relationships among Ocimum basilicum L. Cultivars

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