Assessment of Adrenocortical Activity by Assay of 17-Ketogenic Steroids in Urine

Norymberski, J. K.; Stubbs, R. D.; West, H. F.

doi:10.1016/s0140-6736(53)92414-3

Cited by 283 publications

(53 citation statements)

References 30 publications

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“…Urinary steroids Urinary neutral 17-ketosteroids and 17-ketogenic steroids were determined by a slight modification of the method of Norymberski, Stubbs & West (1953), urinary oestrogens by the method of Brown (1955), as modified by Brown, Bulbrook & Greenwood (1957). Eleonora P. Giorgi…”

Section: Recovery Experiments and Duplicate Determinationmentioning

confidence: 99%

The Determination of Steroids in Cyst Fluid From Human Polycystic Ovaries

Giorgi¹

1963

Journal of Endocrinology

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A micro-method has been developed for the determination of \g=D\4-3-ketosteroids and \g=D\5-3\g=b\-hydroxysteroids,oestradiol-17\g=b\, oestrone and oestriol in small volumes of cyst fluid. The method consists of separation of the neutral and phenolic fractions by solvent partition; fractionation of the neutral steroids by partition and paper chromatography and of the three oestrogens by adsorption chromatography after methylation. This technique has been applied to cyst fluid from the ovaries of twenty-one patients, twelve with the Stein-Leventhal syndrome, and nine with other gynaecological disorders. In the patients with the Stein-Leventhal syndrome the steroid concentrations in cyst fluid showed a definite pattern characterized by high concentrations of \g=D\4-androstenedione, low concentrations of progesterone and 17\g=a\-hydroxyprogesterone, and small amounts or undetectable oestrogens. In cyst fluid from the ovaries of the nine control patients there were, in general, lower concentrations of \g=D\4-androstenedione and significant amounts of oestradiol-17\g=b\.High concentrations of progesterone and oestrogens were found in the cyst fluid of patients with menorrhagia and endometriosis.

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Section: Recovery Experiments and Duplicate Determinationmentioning

confidence: 99%

The Determination of Steroids in Cyst Fluid From Human Polycystic Ovaries

Giorgi¹

1963

Journal of Endocrinology

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“…The content of the l7-KGS in urine was determined with the method of Norymberski & Stubbs (15), as modified by Appleby et al (I) and Jorgensen (12).…”

Section: Methodsmentioning

confidence: 99%

Psychological and biochemical strain in firemen`s work.

Kalimo¹,

Lehtonen²,

M³

et al. 1980

Scand J Work Environ Health

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Psychological and biochemical strain in firemen`s work. by Kalimo R, Lehtonen A, Daleva M, Kuorinka I Key terms: alarm center; ambulance paramedic; biochemical strain; fireman; firemen`s work; job satisfaction; mental strain; psychological strain; psychophysiological stress This article in PubMed: www.ncbi.nlm.nih.gov/pubmed/6937822Scand j work environ health 6 (1980) 6 (1980) 179-187. Mental load and strain in the work of firemen and fire alarm center operators were studied in a group of 260 firemen and 6 female full-time operators from three fire brigades. The firemen proved to be satisfied with their work, and signs of chronic psychological problems were uncommon. Situational work load and related strain may, however, be extreme during active operations. A large proportion (42 0/0) of the firemen suffered from strain after the 24-h long daily work period. Those who had recently worked as paramedics in the ambulance service or in the alarm center were under more strain than ordinary fire fighters. Biochemical stress indicators revealed a high activation of firemen at the alarm center. The difference found between firemen and full-time female operators may be due to a variation in the adaptive functions of the two sexes.Key terms: alarm center, ambulance paramedics, job satisfaction, mental strain, psychophysiological stress.Work-related load and psychological stressors are possible risks to the general well-being of people in various occupations. Congruence between work and man's general needs, expectations, and wishes has proved to be particularly important. A sufficient fit between work demands and man's natural way of functioning is a prerequisite of well-being. It is obvious that work demands may exceed or remain below man's capacity, ie, they may overstimulate or understimulate the working man. On the other hand, suitably challenging and stimulating work offers possi- bilities for developing one's abilities, skills, and personality in a wider sense (2, 7, 11). The special tasks of firemen and their work conditions involve certain particular stress factors, the impact of which on mental and general health has only been scarcely studied (5). Supposedly, firemen might be under considerable stress in their work due to the great responsibility for others' lives, the suddenly occurring rush periods with monotonous intermittent periods on watch, and the fact that it is shift work.In Finland the firemen's labor union took the intiative in studying firemen's work conditions, the mental and physical load of the work, and its effects on the workers' health. Two parts of the project (13) are reported in this communication, namely, the results of a general questionnaire survey covering the total study sample and a more specified study on stress reactions of operators at an alarm center.0355-3140/80/030179-9 QUESTIONNAIRE STUDY Materials and methodsThe subjects of the questionnaire study were 260 firemen from three fire brigades. One of them was the largest in Finland (Helsinki), the second was middle-sized and the...

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“…A modification of the method of Drekter et al (1952) as described by Norymberski, Stubbs & West (1953) was used for the estimation of total 17-oxosteroids. Zimmermann chromogens were measured according to the method of Corker, Norymberski & Thow (1962).…”

Section: Methodsmentioning

confidence: 99%

The indirect analysis of 17-oxosteroid glucosiduronates and sulphates in human urine

Menini¹

1966

Biochemical Journal

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1. An analytical procedure has been developed for the determination of urinary 17-oxosteroid sulphates and 11-deoxy-17-oxosteroid glucosiduronates. 2. By submitting urine to the following operations: (a) extraction of the 17-oxosteroid conjugates into chloroform (in the presence of pyridinium sulphate), (b) solvolysis (in the absence of acid) of the steroid sulphates, (c) separation of the steroid glucosiduronates, (d) hydrolysis of the glucosiduronates, (e) oxidation with tert.-butyl chromate of the liberated steroids and (f) analysis of the final extracts by gas-liquid chromatography, the 17-oxosteroid conjugates are converted into and measured as androstanediones and androstane-(ene)triones. 3. Values for the daily excretion of 17-oxosteroid glucosiduronates and sulphates in normal men and women are given.

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Assessment of Adrenocortical Activity by Assay of 17-Ketogenic Steroids in Urine

Cited by 283 publications

References 30 publications

The Determination of Steroids in Cyst Fluid From Human Polycystic Ovaries

The Determination of Steroids in Cyst Fluid From Human Polycystic Ovaries

Psychological and biochemical strain in firemen`s work.

The indirect analysis of 17-oxosteroid glucosiduronates and sulphates in human urine

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