Assessment of acute toxicological effects of molybdenum(IV) disulfide nano- and microparticles after single intratracheal administration in rats

Sobańska, Zuzanna; Sitarek, Krystyna; Gromadzińska, Jolanta; Świercz, Radosław; Szparaga, Mateusz; Domeradzka-Gajda, Katarzyna; Kowalczyk, K.; Zapór, Lidia; Wąsowicz, Wojciech; Grobelny, Jarosław; Ranoszek-Soliwoda, Katarzyna; Tomaszewska, Emilia; Celichowski, Grzegorz; Roszak, Joanna; Stępnik, Maciej

doi:10.1016/j.scitotenv.2020.140545

Cited by 9 publications

(14 citation statements)

References 32 publications

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“…In our previous study, the most prominent effect after a single intratracheal exposure to MoS 2 nanoparticles and microparticles was the induction of inflammatory changes in the lung tissue, alongside visible deposits of the material in the interstitial tissue and particle-loaded macrophages (Soba nska, Sitarek, et al, 2020). These observations are consistent with the findings of the present study, where inflammatory foci were found in the lungs of the exposed animals.…”

Section: Histopathological Examinationsupporting

confidence: 93%

“…For the STEM analysis, lung and liver tissues were submerged in 10% formalin solution. The samples were rinsed in phosphate buffered saline and fixed in 2% glutaric dialdehyde solution in 130 mM sodium cacodylate buffer solution with 0.1% osmium (VIII)-oxide (OsO 4 ) in water for 6 h and processed as described in an earlier publication (Soba nska, Sitarek, et al, 2020). The sections were imaged using FEI Scios DualBeam™ microscope using the STEM technique.…”

Section: Analysis By Scanning Transmission Electron Microscopy (Stem)...mentioning

confidence: 99%

“…This route of animal exposure has some advantages compared to nose-only inhalation, including its relative simplicity, controlling the exact delivered dose, and not affecting other tissues. This paper is supplementary to our study in which we evaluated acute toxic effects in rats after a single intratracheal exposure to MoS 2 nanoparticles and microparticles (Soba nska, Sitarek, et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

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Biological effects of molybdenum(IV) sulfide nanoparticles and microparticles in the rat after repeated intratracheal administration

Sobańska,

Sitarek,

Gromadzińska

et al. 2023

J of Applied Toxicology

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In this study, molybdenum(IV) sulfide (MoS2) nanoparticles (97 ± 32 nm) and microparticles (1.92 ± 0.64 μm) stabilized with poly (vinylpolypyrrolidone) (PVP) were administered intratracheally to male and female rats (dose of 1.5 or 5 mg/kg bw), every 14 days for 90 days (seven administrations in total). Blood parameters were assessed during and at the end of the study (hematology, biochemistry including glucose, albumins, uric acid, urea, high density lipoprotein HDL, total cholesterol, triglycerides, aspartate transaminase, and alanine transaminase ALT). Bronchoalveolar lavage fluid (BALF) analyses included cell viability, biochemistry (total protein concentration, lactate dehydrogenase, and glutathione peroxidase activity), and cytokine levels (tumor necrosis factor α, TNF‐α, macrophage inflammatory protein 2‐alpha, MIP‐2, and cytokine‐induced neutrophil chemoattractant‐2, CINC‐2). Tissues were subjected to routine histopathological and electron microscopy (STEM) examinations. No overt signs of chronic toxicity were observed. Differential cell counts in BALF revealed no significant differences between the animal groups. An increase in MIP‐2 and a decrease in TNF‐α were observed in BALF in the exposed males. The histopathological changes in the lung evaluated according to a developed classification system (based on severity of inflammation, range 0–4, with 4 indicating the most severe changes) showed average histopathological score of 1.33 for animals exposed to nanoparticles and microparticles at the lower dose, 1.72 after exposure to nanoparticles at the higher dose, and 2.83 for animals exposed to microparticles at the higher dose. In summary, it was shown that nanosized and microsized MoS2 can trigger dose‐dependent inflammatory reactions in the lungs of rats after multiple intratracheal instillation irrespective of the animal sex. Some evidence indicates a higher lung pro‐inflammatory potential of the microform.

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Section: Histopathological Examinationsupporting

confidence: 93%

Section: Analysis By Scanning Transmission Electron Microscopy (Stem)...mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Biological effects of molybdenum(IV) sulfide nanoparticles and microparticles in the rat after repeated intratracheal administration

Sobańska,

Sitarek,

Gromadzińska

et al. 2023

J of Applied Toxicology

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“…The pulmonary toxicity of MoS 2 sheets (with size of 97 nm and 1.9 μm) was also investigated in Sprague−Dawley rats at 1 and 7 days after single intratracheal instillation at 1.5 and 5 mg/kg. 279 BAL fluids demonstrated high level of material internalization in alveolar macrophages, and slightly higher number of neutrophils compared to control at 1 day (resolving by day 7). While cytokines and protein content were not statistically increased in comparison to the vehicle control at day 7, there was a higher lactate dehydrogenase (LDH) content in MoS 2 -treated groups after 1 day, albeit not statistically significant.…”

Section: Impact On the Pulmonary Systemmentioning

confidence: 86%

“…Interestingly, the authors also found such structures in extracellular vesicles in BAL fluid, suggesting a mechanism of inflammation resolution by eliminating the offending material. The pulmonary toxicity of MoS 2 sheets (with size of 97 nm and 1.9 μm) was also investigated in Sprague–Dawley rats at 1 and 7 days after single intratracheal instillation at 1.5 and 5 mg/kg . BAL fluids demonstrated high level of material internalization in alveolar macrophages, and slightly higher number of neutrophils compared to control at 1 day (resolving by day 7).…”

Section: Impact On the Pulmonary Systemmentioning

confidence: 99%

Environmental and Health Impacts of Graphene and Other Two-Dimensional Materials: A Graphene Flagship Perspective

Lin,

Buerki-Thurnherr,

Kaur

et al. 2024

ACS Nano

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Two-dimensional (2D) materials have attracted tremendous interest ever since the isolation of atomically thin sheets of graphene in 2004 due to the specific and versatile properties of these materials. However, the increasing production and use of 2D materials necessitate a thorough evaluation of the potential impact on human health and the environment. Furthermore, harmonized test protocols are needed with which to assess the safety of 2D materials. The Graphene Flagship project (2013−2023), funded by the European Commission, addressed the identification of the possible hazard of graphene-based materials as well as emerging 2D materials including transition metal dichalcogenides, hexagonal boron nitride, and others. Additionally, socalled green chemistry approaches were explored to achieve the goal of a safe and sustainable production and use of this fascinating family of nanomaterials. The present review provides a compact survey of the findings and the lessons learned in the Graphene Flagship.

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Distribution of molybdenum in soft tissues and blood of rats after intratracheal instillation of molybdenum(IV) sulfide nano- and microparticles

Kuraś,

Stępnik,

Grobelny

et al. 2023

Toxicol Res.

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There is still little literature data on the toxicity and safety of the commonly used molybdenum (Mo) disulfide which is present in the working as well as living environments. Thus, an experiment was carried out involving rats, with single and repeated intratracheal exposure (in the latter case, 7 administrations at 2-week intervals with the analysis performed after 90 days) to lower (1.5 mg Mo kg−1 b.w.) and higher (5 mg Mo kg−1 b.w.) doses of molybdenum(IV) sulfide nanoparticles (MoS2-NPs) and microparticles (MoS2-MPs). The analysis of Mo concentrations in the tail and heart blood as well as in soft tissues (lung, liver, spleen, brain), after mineralization and bioimaging, was meant to facilitate an assessment of its accumulation and potential effects on the body following short- and long-term exposure. The multi-compartment model with an exponential curve of Mo concentration over time with different half-lives for the distribution and elimination phases of MoS2-MPs and MoS2-NPs was observed. After 24 h of exposure, a slight increase in Mo concentration in blood was observed. Next, Mo concentration indicated a decrease in blood concentration from 24 h to day 14 (the Mo concentration before the second administration), below the pre-exposure concentration. The next phase was linear, less abrupt and practically flat, but with an increasing trend towards the end of the experiment. Significantly higher Mo concentrations in MoS2-NPs and MoS2-MPs was found in the lungs of repeatedly exposed rats compared to those exposed to a single dose. The analysis of Mo content in the liver and the spleen tissue showed a slightly higher concentration for MoS2-NPs compared to MoS2-MPs. The results for the brain were below the calculated detection limit. Results were consistent with results obtained by bioimaging technique.

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Assessment of acute toxicological effects of molybdenum(IV) disulfide nano- and microparticles after single intratracheal administration in rats

Cited by 9 publications

References 32 publications

Biological effects of molybdenum(IV) sulfide nanoparticles and microparticles in the rat after repeated intratracheal administration

Biological effects of molybdenum(IV) sulfide nanoparticles and microparticles in the rat after repeated intratracheal administration

Environmental and Health Impacts of Graphene and Other Two-Dimensional Materials: A Graphene Flagship Perspective

Distribution of molybdenum in soft tissues and blood of rats after intratracheal instillation of molybdenum(IV) sulfide nano- and microparticles

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