Assessment of acute toxicity of the organomineral chelate complex for Danio rerio embryos

Abstract

The work evaluates the acute toxicity of the organomineral chelate complex for Danio rerio embryos. To date, very few studies have been presented on the mineral component of diets in aquaculture.Currently, a number of preparations (supplements) containing microelements are used to correct mineral feeding in livestock farming. A number of studies have shown that the use of organomineral chelate complexes as a feed additive provides better assimilation of the metal than when it is introduced into the diet in inorganic or any other organic form.The domestic market does not have scientifically based organomineral chelate complexes for industrial aquaculture. The search for effective and non-toxic chelate complexes for aquaculture in the Russian Federation is an urgent task. Purpose of the work: to determine the median lethal concentration of the organomineral chelate complex on Danio rerio embryos. The acute toxicity of an organomineral chelate complex (JUPITER LLC, Russia) was determined. The active basis of the dietary supplement (hereinafter referred to as dietary supplement): a complex of ethylenediaminedisuccinic acid and lysine with microelements (Fe, Mn,Cu, Zn, Co, Se, I). The studies were carried out in accordance with OECD recommendations No. 236 on the basis of the Department of Biochemistry and Physiology of the St. Petersburg State University of Veterinary Medicine. Based on the study, the following conclusions can be drawn: the maximum concentration that does not cause death during testing is 0.25 g/l and the minimum concentration that causes 100% death during testing is 16 g/l. Based on the results of the survival of Danio rerio embryos, the median lethal concentration of the organomineral chelate complex was calculated. The LC50 of the study drug is 4.67 g/l.