Assessment of a transcervical aspiration technique for chorionic villus biopsy in the first trimester of pregnancy.

Horwell, David; Loeffler, F. E.; Coleman, D. V.

doi:10.1111/j.1471-0528.1983.tb08607.x

Cited by 49 publications

(10 citation statements)

References 4 publications

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“…In our previous study using a 'blind' technique, the figures were 58% and 33% respectively. 2 ) This figure is similar to that reported by Horwell et al, 1983.1) Simoni et al, 1983, 6 ) however only noticed bleeding in less than 5% of their patients but the degree of bleeding is not stated. The majority of our observed bleeding was considered only minimal.…”

Section: Discussionsupporting

confidence: 84%

Experience with Real Time Ultrasound Directed Transcervical Chorionic Villus Biopsy

Liu

Norman

Richardson

et al. 1985

Asia-Oceania Journal of Obstetrics and Gynaecology

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The introduction of real time ultrasound to transcervical chorionic villus biopsy allowed successful collection from 65% of 107 consecutive studied patients. The collection rate was obtained despite the need for time to develop scanning skills and a limit of only two attempts at biopsy. The quantity in 69% of biopsy samples was· considered sufficient for use in prenatal diagnosis. Biopsy was most successful between 8 and 10 weeks gestation. No amniotic sac was perforated. Bleeding of varying severity associated with 65% of biopsies was not improved despite use of ultrasound. Both linear array or sector scanners were suitable for our purpose. Clarity of the scan picture is affected by many factors. Successful biopsy is not always dependent on visualisation of the placenta or tip of the collecting cannula. Apart from expertise and ultrasound guidance, better design in biopsy implements can improve successful collection to levels acceptable for clinical applications.

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Section: Discussionsupporting

confidence: 84%

Experience with Real Time Ultrasound Directed Transcervical Chorionic Villus Biopsy

Liu

Norman

Richardson

et al. 1985

Asia-Oceania Journal of Obstetrics and Gynaecology

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“…The availability of pure viable cytotrophoblasts provides a good starting point for such studies and also facilitates immunological, endocrinological, and molecular studies of human trophoblasts. This method may also provide the means to identify and collect cytotrophoblasts by transcervical chorion biopsy or possibly in maternal peripheral blood (49,50) for direct biochemical and molecular diagnoses at early prenatal stages.…”

Section: Discussionmentioning

confidence: 99%

Transcriptional control of HLA-A,B,C antigen in human placental cytotrophoblast isolated using trophoblast- and HLA-specific monoclonal antibodies and the fluorescence-activated cell sorter.

Kawata¹,

Parnes²,

Herzenberg³

1984

The Journal of Experimental Medicine

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Human placental cell suspensions prepared by trypsin digestion were analyzed with several monoclonal antibodies on a multiparameter fluorescence-activated cell sorter (FACS). Five distinct cell populations were isolated on the basis of size and quantitative differences in the coordinate expression of cell surface antigens detected by monoclonal antibodies against an HLA-A,B,C monomorphic determinant (MB40.5) and against human trophoblasts (anti-Trop-2). By FACS analysis and after sorting we clearly identified the major cell population as cytotrophoblasts based on several independent criteria, including presence of trophoblast-specific surface antigens, Trop-1, and Trop-2; absence of all HLA class I, class II, and beta 2-microglobulin (beta 2m) antigens; absence of the pan-leucocyte and monocyte antigens, HLe1 and LeuM1, respectively; presence of Y-chromatin in a male placenta; presence of placental and not liver alkaline phosphatase; and a large, mononuclear morphology. These procedures provide a reproducible method for obtaining highly purified human cytotrophoblast populations for further studies. We measured by molecular hybridization (RNA or Northern blots) the HLA-A,B,C and beta 2m mRNA in total RNA extracted from sorted cytotrophoblasts. We find that normal human cytotrophoblasts have extremely small amounts of HLA-A,B,C mRNA: approximately 300 times less than that in the lymphoid cell line LCL-721 or normal lymphocytes. In contrast, they have approximately 11% the level of beta 2m mRNA present in LCL-721 cells. Thus, HLA-A,B,C antigen expression on human cytotrophoblasts is limited by the level of HLA heavy chain mRNA.

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“…Prenatal diagnosis using chorionic villus sampling (CVS) has been recently developed to serve in place of midtrimester amniocentesis. It has spread mainly in Europe, and promises to become a broad index of usefulness in the United States (Simmoni et al, 1983;Horwell et al, 1983;Kolata, 1983;Cowart, 1984;Modell, 1985;Perry et al, 1985;Elias et al, 1985). CVS has the advantages of permitting diagnosis during early pregnancy, and even should fetal abnormality be detected, the psychological and physical impacts on a mother opting for therapeutic abortion are minimal.…”

Section: Chorionic Villus Samplingmentioning

confidence: 99%

Prenatal Diagnosis of Fetal Chromosomes: Evaluation of Mi dtri mester Amniocetesis and Chorionic Villus Sampling in Early Pregnancy*

Suzumori

1985

Congenital Anomalies

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The congenital anomaly resulting from chromosomal aberrations is serious and well‐known. The incidence in newborn infants is estimated to be 0.5‐1% (WHO). In many cases the mechanism causing a chromosomal aberration is unknown. There appear to be the protective procedures. The procedure that fetal cells obtained by midtrimester amniocentesis could be grown in culture and karyotyped has opened a new area to genetic counseling for chromosomal aberrations. In the past 15 years, 949 pregnant women have undergone amniocentesis in our hospital. Most institutes now employ ultrasonography for examinations of the fetal position and the localization of placenta prior to amniocentesis. The use of this device remarkably reduced the incidence of fetal loss, and the amniocentesis appears to be safe and efficient. A discrepancy has been noted between the rates of chromosomal aberration by diagnosed at amniocentesis and the rates that would be expected from observation in live births. Multiple regression analysis was performed based on the personal informations of mother, providing what may be the risk factors for the recurrence of sibling with a non‐inhetrited chromosomal aberration. Recently, the technique to obtain chorionic villus in early pregnancy for the prenatal diagnosis of fetal defects, now being tested in the United States and Europe, may replace midtrimester amniocentesis within the next few years. The main advantage is that it can reduce the psychologic impact because emotional and physical stresses of a midtrimester abortion are serious problems. There are many possible approaches to obtain chorionic villus sample. Our experience has shown that biopsy forceps inserted via the cervix is most successful. The possibility was discussed that this may be developed in the future for clinical use as an alternative to amniocentesis.

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Assessment of a transcervical aspiration technique for chorionic villus biopsy in the first trimester of pregnancy.

Cited by 49 publications

References 4 publications

Experience with Real Time Ultrasound Directed Transcervical Chorionic Villus Biopsy

Experience with Real Time Ultrasound Directed Transcervical Chorionic Villus Biopsy

Transcriptional control of HLA-A,B,C antigen in human placental cytotrophoblast isolated using trophoblast- and HLA-specific monoclonal antibodies and the fluorescence-activated cell sorter.

Prenatal Diagnosis of Fetal Chromosomes: Evaluation of Mi dtri mester Amniocetesis and Chorionic Villus Sampling in Early Pregnancy*

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