Assessment of a pro-healing stent in an animal model of early neoatherosclerosis

Abstract

Background: neoatherosclerosis represents an accelerated manifestation of atherosclerosis in nascent neointima after stenting, associated with adverse events. We investigated whether improved reendothelialization using RGD-coated stents results in diminished vascular permeability and reduced foam cell formation compared to standard DeS in atherosclerotic rabbits. Methods and Results: neointimal foam cell formation was induced in rabbits (n = 7). Enhanced endothelial integrity in RGDcoated stents resulted in decreased vascular permeability relative to DES, which was further confirmed by SEM and TEM. Cell culture experiments examined the effect of everolimus on endothelial integrity. increasing concentrations of everolimus resulted in a dose-dependent decrease of endothelial cell junctions and foam cell transformation of monocytes, confirming the relevance of endothelial integrity in preventing permeability of LDL. Conclusion: Incomplete endothelial integrity was confirmed as a key factor of neointimal foam cell formation following stent implantation. pro-healing stent coatings may facilitate reendothelialization and reduce the risk of neoatherosclerosis.

Restoring endothelial function: shedding light on cardiovascular stent development

“…Joner et al investigated whether cyclic arginine-glycine-aspartic acid (cRGD)-coated stents that targeted integrin α v β 3 could increase the adhesion of ECs as well as elevate endothelial barrier function. 154 The results indicated that cRGD-coated stents showed superiority in reinforcing endothelial integrity and maintaining the semi-permeability compared to commercial DES (Fig. 10B).…”

Global research trends in in-stent neoatherosclerosis: A CiteSpace-based visual analysis

“…New Zealand White (NZW) rabbits are widely used as an animal model for the study of atherosclerosis. In rabbits, balloon dilation and stenting of major arteries such as the common carotid artery, abdominal aorta, and iliac artery can be used to study the mechanism of injury in ISNA research (63)(64)(65)(66). In addition, implantation of coronary stents in the abdominal aorta of apoE-/-rats is a good model for studying ISR after stent placement and can potentially be used to investigate the mechanisms of injury associated with ISNA (67).…”