Assessment of a dimethyl sulfoxide–stabilized frozen canine platelet concentrate

Guillaumin, Julien; Jandrey, Karl E.; Norris, Jeffrey W.; Tablin, Fern

doi:10.2460/ajvr.69.12.1580

Cited by 26 publications

(33 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…48 This study identified decreases in platelet number and function as a result of the freeze-and-thaw process. 48 This study identified decreases in platelet number and function as a result of the freeze-and-thaw process.…”

Section: Cryopreserved Canine Platelet Concentratementioning

confidence: 84%

Blood Transfusion and Blood Substitutes

Hohenhaus¹

2012

Fluid, Electrolyte, and Acid-Base Disorders in Small Animal Practice

View full text Add to dashboard Cite

Section: Cryopreserved Canine Platelet Concentratementioning

confidence: 84%

Blood Transfusion and Blood Substitutes

Hohenhaus¹

2012

Fluid, Electrolyte, and Acid-Base Disorders in Small Animal Practice

View full text Add to dashboard Cite

“…The presence of the platelets in the samples likely increased clot strength through their physical integration into the forming clot and by providing additional phosphatidylserine expressing surfaces for the assembly of coagulation factor complexes thereby accelerating clot formation . It is likely that few of the platelets survived the freeze/thaw process despite the presence of the dimethyl sulfoxide . At the time of collection, our PRP samples contained a high number of platelets in an attempt to account for the anticipated loss due to the freeze/thaw process.…”

Section: Discussionmentioning

confidence: 99%

Multicenter in vitro thromboelastography and thromboelastometry standardization

Goggs

Borrelli

Brainard

et al. 2018

J Vet Emergen Crit Care

Self Cite

View full text Add to dashboard Cite

More variation within and between centers was identified than anticipated, but some parameters such as alpha angle were repeatable and reproducible. Sample types for future multicenter standardization efforts will require further optimization and may need to be adapted separately to each platform. Individual centers using viscoelastic tests for evaluation and management of clinical patients should take steps to minimize preanalytical and analytical sources of variation.

show abstract

“…Because of their ease of use in clinical settings and apparent efficacy in this case series, the authors consider cryo‐PRP an appropriate option for managing CSS. After freeze‐thaw, canine cryo‐preserved platelets externalize PS . It is possible that these platelets provide an advantage to fresh platelets in promoting thrombin generation in CSS patients.…”

Section: Discussionmentioning

confidence: 99%

“…After freeze-thaw, canine cryo-preserved platelets externalize PS. 17 It is possible that these platelets provide an advantage to fresh platelets in promoting thrombin generation in CSS patients. However, the shorter half-life of cryopreserved platelets may necessitate higher doses or repeated transfusion versus fresh PRP.…”

Section: Discussionmentioning

confidence: 99%

Clinical Characterization of Canine Platelet Procoagulant Deficiency (Scott Syndrome)

Jandrey

Norris

Tucker

et al. 2012

Veterinary Internal Medicne

View full text Add to dashboard Cite

Background Platelet function defects are rare causes of bleeding diatheses; however, disease prevalence might be underestimated because diagnosis requires assessment of specific parameters of platelet activation. Objectives The goal of this study was to characterize the clinical presentation of canine Scott syndrome (CSS), an intrinsic platelet function defect first identified in a closed colony of German Shepherds (GSD). Animals Eleven (n = 6 female) client‐owned GSD affected with CSS that sought veterinary care for one or more episodes of abnormal bleeding. Methods Retrospective review of all cases of CSS diagnosed through the Comparative Coagulation Laboratory at Cornell University between 2005 and 2011. The diagnosis of CSS was based on 2 measures of platelet procoagulant activity: serum prothrombin consumption and flow cytometric detection of platelet phosphatidylserine externalization after in vitro activation. Results Postoperative hemorrhage was the most common sign of CSS, whereas petechiae were not found in any dog. Although most GSD responded to platelet transfusion, refractory epistaxis in 2 GSD was managed by nasal arterial embolization. The CSS trait was not restricted to a single pedigree of related GSD or to a single geographic region. Conclusions and Clinical Importance Unlike thrombocytopenia and platelet aggregation defects, petechiae and other capillary hemorrhage are not typical features of CSS. After preliminary screening to rule out more common causes of hemorrhage, CSS should be considered in the differential diagnosis of recurrent hemorrhage in GSD, and potentially other breeds of dog. Definitive diagnosis of CSS requires specific tests of platelet procoagulant activity.

show abstract

Assessment of a dimethyl sulfoxide–stabilized frozen canine platelet concentrate

Abstract: Results of this in vitro study indicated that there was a decrease in platelet quantity and function as well as an increase in platelet activation during the freeze-and-thaw process in DMSO-stabilized canine frozen PC. In vivo effects on PC remain to be determined.

Cited by 26 publications

References 28 publications

Blood Transfusion and Blood Substitutes

Blood Transfusion and Blood Substitutes

Multicenter in vitro thromboelastography and thromboelastometry standardization

Clinical Characterization of Canine Platelet Procoagulant Deficiency (Scott Syndrome)

Contact Info

Product

Resources

About