Assessment of a Commercially Available Serum Pregnancy-Specific Protein B Test in Free-Ranging Elk (Cervus canadensis) in Pennsylvania, USA

Seixas, Julia Silva; Jayarao, Bhushan M.; Banfield, Jeremiah E.; Johnson, Joshua B.; Brown, Justin D.

doi:10.7589/2018-11-282

Cited by 4 publications

(3 citation statements)

References 7 publications

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“…Pregnancy may increase glucocorticoid levels during gestation and the binding globulin for progesterone also has a strong affinity for glucocorticoids (Breuner and Orchinik 2002). Therefore, we determined pregnancy status for each female elk via an ELISA for serum pregnancy‐specific protein B (PSPB; Noyes et al 1997, Seixas et al 2019) from blood samples collected from each female postmortem. Lactation was assessed by palpating the udder and visually confirming presence of milk at the check station.…”

Section: Methodsmentioning

confidence: 99%

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The influence of hunting pressure and ecological factors on fecal glucocorticoid metabolites in wild elk

et al. 2020

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Section: Methodsmentioning

confidence: 99%

“…We present results as model coefficient estimates (Estimates) ± 1 SE with 95% confidence intervals (CI). Lastly, as PSPB has lower sensitivity during early gestation (Seixas et al 2019) and neither pregnancy nor lactation altered the model selection (ΔAIC >100), we removed them from further analysis.…”

Section: Methodsmentioning

confidence: 99%

The influence of hunting pressure and ecological factors on fecal glucocorticoid metabolites in wild elk

et al. 2020

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“…Pregnancy-specific protein B (PSPB), a protein hormone secreted by the placenta in ruminants, may be used to determine pregnancy with high accuracy in many species, including elk ( Cervus canadensis ; Noyes et al 1997; Seixas et al 2019) and white-tailed deer ( Odocoileus virginianus ; Osborn et al 1995). However, PSPB must be measured from a blood sample, requiring capture.…”

Section: Introductionmentioning

confidence: 99%

Evaluating the Efficacy of Noninvasive Fecal Sampling for Pregnancy Detection in Elk (Cervus Canadensis)

Watson

Kurth

Metts

et al. 2023

Journal of Wildlife Diseases

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Elk (Cervus canadensis) were reintroduced to Tennessee, USA in the early 2000s, with limited reproductive monitoring since initial release. We assessed the efficacy of noninvasive sampling for determining pregnancy using invasive (capture) and noninvasive (fecal collection in the field) techniques at the North Cumberland Wildlife Management Area (NCWMA), Tennessee. We captured 20 female elk 2019–2020, used pregnancy-specific protein B (PSPB) in blood to determine pregnancy and compared results to fecal progesterone metabolite (FPM) concentrations using two commercially available enzyme immunoassay (EIA) kits. Based on PSPB concentrations, 8/11 and 3/4 of captured adult elk (≥2.5 yr of age) were pregnant in 2019 and 2020, respectively; no 1.5-yr-old elk were pregnant (n=5). Using the progesterone EIA kit, FPM concentrations were x̄=192.84±38.63 ng/g (95% CI, 96.48–289.20) for nonpregnant and x̄=536.17±74.98 ng/g (95% CI, 375.97–696.36) for pregnant captured females. For the progesterone metabolite kit, FPM concentrations were x̄=188.16±43.39 ng/g (95% confidence interval [CI], 76.63–299.69) for nonpregnant and x̄=693.52±126.52 ng/g (95% CI, 407.31–979.72) for pregnant captured females. From February to May 2019, we collected 357 fecal samples in 65 areas across 489.62 km2 of the NCWMA. Using extracted DNA and analysis of 15 microsatellites, we identified 62 unique individuals from 128 female fecal samples collected on the landscape. We categorized females from landscape-collected feces as nonpregnant (35.5–40.3%; Metabolite-EIA kits), undetermined (1.6–6.5%; Metabolite-EIA kits), or pregnant (62.9–53.2%; Metabolite-EIA kits) based on a 95% CI of captured female FPM concentrations, giving an overall pregnancy rate of 53.2% using the recommended EIA kit. The pregnancy rate in sexually mature females may be higher, as it was not possible to distinguish age classes of landscape-collected fecal samples; therefore, some may have been from younger age classes not expected to be pregnant. Analysis of FPM may be useful at a population level to detect pregnancy.

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Evaluating the utility of harvester-collected samples for muskox (Ovibos moschatus) pregnancy diagnostics

Hee,

Pruvot,

Mavrot

et al. 2024

Theriogenology Wild

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Assessment of a Commercially Available Serum Pregnancy-Specific Protein B Test in Free-Ranging Elk (Cervus canadensis) in Pennsylvania, USA

Cited by 4 publications

References 7 publications

The influence of hunting pressure and ecological factors on fecal glucocorticoid metabolites in wild elk

The influence of hunting pressure and ecological factors on fecal glucocorticoid metabolites in wild elk

Evaluating the Efficacy of Noninvasive Fecal Sampling for Pregnancy Detection in Elk (Cervus Canadensis)

Evaluating the utility of harvester-collected samples for muskox (Ovibos moschatus) pregnancy diagnostics

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